In vitro osteoblast-like cell metabolism in spondylodesis - A tool that may predict fusion capacity: A prospective study in 50 patients with a 1-year follow-up

Acta Orthopaedica Scandinavica

Volumn 74, Issue 6, 2003, Pages 730-736

  Laursen, Malene ^a   Christensen, Finn B ^a   Lind, Martin ^a   Hansen, Ebbe S ^a   Høy, Kristian ^a   Gelineck, John ^a   Bünger, Cody ^a  

^a AARHUS UNIVERSITY HOSPITAL   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; PROCOLLAGEN; TRITIUM;

ANTERIOR SPINE FUSION; ARTICLE; BONE BIOPSY; BONE MATRIX; CELL GROWTH; CELL METABOLISM; CONTROLLED STUDY; ENZYME ACTIVITY; EVALUATION; EXPLANT; HUMAN; HUMAN CELL CULTURE; HUMAN TISSUE; ILIAC CREST; IN VITRO STUDY; LUMBAR SPINE; OSTEOBLAST; PROTEIN EXPRESSION; SPONDYLODESIS;

ADULT; AGED; ALKALINE PHOSPHATASE; CELLS, CULTURED; COLLAGEN TYPE I; FEMALE; FOLLOW-UP STUDIES; HUMANS; MALE; MIDDLE AGED; OSTEOBLASTS; PREDICTIVE VALUE OF TESTS; PROSPECTIVE STUDIES; SMOKING; SPINAL FUSION; TREATMENT OUTCOME;

EID: 0346340839     PISSN: 00016470     EISSN: None     Source Type: Journal    
DOI: 10.1080/00016470310018289     Document Type: Article

References (28)

1. Bellows C G, Heersche J N, Aubin J E. Determination of the capacity for proliferation and differentiation of osteoprogenitor cells in the presence and absence of dexamethasone. Dev Biol 1990; 140: 132-8.
2. Capen D A, Calderone R R, Green A. Perioperative risk factors for wound infection after lower back fusions. Orthop Clin North Am 1996; 27: 83-6.
3. Chavassieux P M, Chenu C, Valentin-Opran A, Merle B, Delmas P D, Hartmann D J, Saez S, Meunier P J. Influence of experimental conditions on osteoblast activity in human primary bone cell cultures. J Bone Miner Res 1990; 5: 337-43.
4. Christensen F B, Lind M, Eiskjaer S P, Thomsen K, Hansen E S, Bunger C E. Can autologous bone culture predict spinal fusion capacity? Eur Spine J 1999; 8: 54-60.
5. Christensen F B, Laursen M, Gelineck J, Eiskjaer S P, Thomsen K, Bünger C. Interobserver and intraobserver agreement of radiographic interpretation with and without pedicle screw implants: The need for a detailed classification system in posterolateral spinal fusion. Spine 2001; 26: 538-44.
6. Davy D T. Biomechanical issues in bone transplantation. Orthop Clin North Am 1999; 30: 553-63.
7. D'Ippolito G, Schiller P C, Ricordi C, Roos B A, Howard G A. Age-related osteogenic potential of mesenchymal stromal stem cells from human vertebral bone marrow. J Bone Miner Res 1999; 14: 1115-22.
8. Evans C E, Galasko C S, Ward C. Effect of donor age on the growth in vitro of cells obtained from human trabecular bone. J Orthop Res 1990; 8: 234-7.
9. Fedarko N S, Bianco P, Vetter U, Robey P G. Human bone cell enzyme expression and cellular heterogeneity: correlation of alkaline phosphatase enzyme activity with cell cycle. J Cell Physiol 1990; 144: 115-21.
10. Fedarko N S, Vetter U K, Weinstein S, Robey P G. Age-related changes in hyaluronan, proteoglycan, collagen, and osteonectin synthesis by human bone cells. J Cell Physiol 1992; 151: 215-27.
11. Gibson J A, Grant I C, Waddell G. The Cochrane review of surgery for lumbar disc prolapse and degenerative lumbar spondylosis. Spine 1999; 24 (17): 1820-32.
12. Gould S E, Rhee J M, Tay B K B, Otsuka N Y, Bradford D S. Cellular contribution of bone graft to fusion. J Orthop Res 2000; 18 (6): 920-7.
13. Kassem M, Ankersen L, Eriksen E F, Clark B F, Rattan S I. Demonstration of cellular aging and senescence in serially passaged long-term cultures of human trabecular osteoblasts. Osteoporos Int 1997; 7: 514-24.
14. Katzburg S, Lieberherr M, Ornoy A, Klein B Y, Hendel D, Somjen D. Isolation and hormonal responsiveness of primary cultures of human bone-derived cells: gender and age differences. Bone 1999; 25: 667-73.
15. Koshihara Y, Honda Y. Age-related increase in collagen production in cultured human osteoblast-like periosteal cells. Mech Ageing Dev 1994; 74: 89-101.
16. Kudelska-Mazur D, Lewandowska-Szumiel M, Komender J. Human osteoblast in contact with various biomaterials in vitro. Ann Transplant 1999; 4: 98-100.
17. Laursen M, Christensen F B, Hansen E S, Hoy K, Gelineck J, Niedermann B, Lind M, Bünger C E. Fusion rates in posterolateral spondylodesis depend on the quantity of autograft applied. (Submittet 2002)
18. Lian J B, Stein G S. Development of the osteoblast phenotype: molecular mechanisms mediating osteoblast growth and differentiation. Iowa Orthop J 1995; 15: 118-40.
19. Matsuyama T, Lau K H, Wergedal J E. Monolayer cultures of normal human bone cells contain multiple subpopulations of alkaline phosphatase positive cells. Calcif Tissue Int 1990; 47: 276-83.
20. Pfeilschifter J, Diel I, Pilz U, Brunotte K, Naumann A, Ziegler R. Mitogenic responsiveness of human bone cells in vitro to hormones and growth factors decreases with age. J Bone Miner Res 1993; 8: 707-17.
21. Rattner A, Sabido O, Massoubre C, Rascle F, Frey J. Characterization of human osteoblastic cells: influence of the culture conditions. In Vitro Cell Dev Biol Anim 1997; 33: 757-62.
22. Robey P G, Termine J D. Human bone cells in vitro. Calcif Tissue Int 3 1985; 7: 453-60.
23. Schmidt R, Kulbe K D. Long-term cultivation of human osteoblasts. Bone Miner 1993; 20: 211-21.
24. Siggelkow H, Rebenstorff K, Kurre W, Niedhart C, Engel I, Schulz H, Atkinson M J, Hufner M. Development of the osteoblast phenotype in primary human osteoblasts in culture: comparison with rat calvarial cells in osteoblast differentiation. J Cell Biochem 1999a; 75: 22-35.
25. Siggelkow H, Schulz H, Kaesler S, Benzler K, Atkinson M J, Hufner M. 1, 25 dihydroxyvitamin-D3 attenuates the confluence-dependent differences in the osteoblast characteristic proteins alkaline phosphatase, procollagen I peptide, and osteocalcin. Calcif Tissue Int 1999b; 64: 414-21.
26. Sutherland M S, Rao L G, Muzaffar S A, Wylie J N, Wong M M, McBroom R J, Murray T M. Age-dependent expression of osteoblastic phenotypic markers in normal human osteoblasts cultured long-term in the presence of dexamethasone. Osteoporos Int 1995; 5: 335-43.
27. Termine J D. Cellular activity, matrix proteins, and aging bone. Exp Gerontol 1990; 25: 217-21.
28. Thalgott J S, Cotler H B, Sasso R C, LaRocca H. Gardner V. Postoperative infections in spinal implants. Classification and analysis - a multicenter study. Spine 1991; 16: 981-4.