Tryptophan Phosphorescence Spectroscopy Reveals That a Domain in the NAD(H)-binding Component (dI) of Transhydrogenase from Rhodospirillum rubrum Has an Extremely Rigid and Conformationally Homogeneous Protein Core

Journal of Biological Chemistry

Volumn 278, Issue 48, 2003, Pages 47578-47584

  Broos, Jaap ^a   Gabellieri, Edi ^b   Van Boxel, Gijs I ^c   Jackson, J Baz ^c   Strambini, Giovanni B ^b  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b CNR Consiglio Nazionale delle Ricerche   (Italy)

^c UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; COMPLEXATION; CONFORMATIONS; ENZYMES; HYDRIDES; PHOSPHORESCENCE; PROTONS; QUENCHING; SPECTROSCOPY;

COFACTOR BINDING;

BIOCHEMISTRY;

NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) TRANSHYDROGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TRYPTOPHAN;

AMINO ACID SUBSTITUTION; ARTICLE; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; DIMERIZATION; ENVIRONMENTAL TEMPERATURE; ENZYME CONFORMATION; ENZYME MECHANISM; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTON TRANSPORT; RHODOSPIRILLUM RUBRUM; SPECTROSCOPY; TRYPTOPHAN PHOSPHORESCENCE SPECTROSCOPY;

ACRYLAMIDE; DIMERIZATION; ESCHERICHIA COLI; GLYCEROL; KINETICS; LUMINESCENT MEASUREMENTS; MODELS, MOLECULAR; NAD; NADP; NADP TRANSHYDROGENASE; OXIDATION-REDUCTION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; PROTONS; RHODOSPIRILLUM RUBRUM; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY; TEMPERATURE; TIME FACTORS; TRYPTOPHAN;

BACTERIA (MICROORGANISMS); RHODOSPIRILLUM; RHODOSPIRILLUM RUBRUM;

EID: 0346220290     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M309287200     Document Type: Article

References (33)

1. Jackson, J. B., White, S. A., Quirk, P. G., and Venning, J. D. (2002) Biochemistry 41, 4173-4185
2. Buckley, P. A., Jackson, B. J., Schneider, T., White, S. A., Rice, D. W., and Baker, P. J. (2000) Struct. Fold. Des. 8, 809-815
3. Prasad, G. S., Wahlberg, M., Sridhar, V., Sundaresan, V., Yamaguchi, M., Hatefi, Y., and Stout, C. D. (2002) Biochemistry. 41, 12745-12754
4. White, S. A., Peake, S. J., McSweeney, S., Leonard, G., Cotton, N. P., and Jackson, J. B. (2000) Struct. Fold. Des. 8, 1-12
5. Prasad, G. S., Sridhar, V., Yamaguchi, M., Hatefi, Y., and Stout, C. D. (1999) Nat. Struct. Biol. 6, 1126-1131
6. Cotton, N. P., White, S. A., Peake, S. J., McSweeney, S., and Jackson, J. B. (2001) Structure 9, 165-176
7. Phelps, D. C., and Hatefi, Y. (1984) Biochemistry 23, 4475-4480
8. Diggle, C., Bizouarn, T., Cotton, N. P., and Jackson, B. J. (1996) Eur. J. Biochem. 241, 162-170
9. Diggle, C., Hutton, M., Jones, G. R., Thomas, C. M., and Jackson, J. B. (1995) Eur. J. Biochem. 228, 719-726
10. Galley, W. C. (1976) in Biochemical Fluorescence; Concepts (Chen, R. F., and Edelhoch, H., eds) pp. 409-439, Dekker New York
11. Hershberger, M. V., Maki, A. H., and Galley, W. C. (1980) Biochemistry 19, 2204-2209
12. Strambini, G. B., and Gonnelli, M. (1995) J. Am. Chem. Soc. 117, 7646-7651
13. Gonnelli, M., and Strambini, G. B. (1995) Biochemistry 34, 13847-13857
14. Cioni, P., and Strambini, G. B. (1998) J. Am. Chem. Soc. 120, 11749-11757
15. Strambini, G. B., and Cioni, P. (1999) J. Am. Chem. Soc. 121, 8337-8344
16. Cioni, P., and Strambini, G. B. (1989) J. Mol. Biol 207, 237-247
17. Strambini, G. B., and Gonnelli, M. (1990) Biochemistry 29, 196-203
18. Strambini, G. B., Cioni, P., Peracchi, A., and Mozzarelli, A. (1992) Biochemistry 31, 7535-7542
19. Gabellieri, E., Rahuel Clermont, S., Branlant, G., and Strambini, G. B. (1996) Biochemistry 35, 12549-12559
20. Schauerte, J. A., Steel, D. G., and Gafni, A. (1999) Methods Enzymol. 278, 49-71
21. Cioni, P., and Strambini, G. B. (1994) J. Mol. Biol. 242, 291-301
22. Strambini, G. B., and Gabellieri, E. (1996) Biophys. J. 70, 971-976
23. van Boxel, G. I., Quirk, P. G., Cotton, N. P., White, S. A., and Jackson, J. B. (2003) Biochemistry 42, 1217-1226
24. Strambini, G. B., and Gabellieri, E. (1991) J. Phys. Chem. 95, 4347-4352
25. Kerwin, B. A., Chang, B. S., Gegg, C. V., Gonnelli, M., Li, T., and Strambini, G. B. (2002) Protein Sci. 11, 1825-1833
26. Quirk, P. G., Jeeves, M., Cotton, N. P., Smith, J. K., and Jackson, B. J. (1999) FEBS Lett. 446, 127-132
27. Rodrigues, D. J., Venning, J. D., Quirk, P. G., and Jackson, J. B. (2001) Eur. J. Biochem. 268, 1430-1438
28. Cioni, P., Gabellieri, E., Gonnelli, M., and Strambini, G. B. (1994) Biophys. Chem. 52, 25-34
29. Vanderkooi, J. M. (1992) in Topics in Fluorescence Spectroscopy (Lakowicz, J. R., ed) pp. 113-136, Plenum, New York
30. Strambini, G. B., Cioni, P., and Puntoni, A. (1989) Biochemistry 28, 3808-3814
31. Quirk, P. G., Smith, K. J., Thomas, C. M., and Jackson, J. B. (1999) Biochim. Biophys. Acta 1412, 139-148
32. Venning, J. D., Rodrigues, D. J., Weston, C. J., Cotton, N. P., Quirk, P. G., Errington, N., Finet, S., White, S. A., and Jackson, J. B. (2001) J. Biol. Chem. 276, 30678-30685
33. Kraulis, P. J. (1991) J. Appl. Crystallogr. 24, 946-950