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Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
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2 wafers at 400° to 500°C, depending on particle size distribution, was followed by sonication in 1:1 water and ethanol. The suspension of annealed wires was drop coated onto TEM grids for visualization. Increasing the wetability of the substrates was necessary for the alignment of the virus assemblies.
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26
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0348201478
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note
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2). Samples were prepared by centrifugation of the virus-particle suspension into 1-mg pellets and allowed to dry.
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27
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0346940836
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note
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TEM samples were analyzed with the JEOL 2010 and 2010-Field Emission Gun (FEG) microscopes. HAADF analysis of the ZnS and CdS samples was performed on the JEOL 2010-FEG. Gantan Digital Micrograph is used to subtract the background of the HRTEM lattice images. Thermal analysis of the CoPt system was performed in situ on the JEOL 200CX microscope operated at 200 kV, with a Gatan heat stage.
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0348201476
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2) to prevent the onset of oxidation for 3 hours at 350°C.
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36
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0347571751
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note
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The virus assembly was reconstructed from the gP8 protein structure obtained from the Protein Data Bank (PDB) (number 1ifj) by application of the appropriate translation vectors. A random number generator was used to realistically incorporate peptide inserts in the gP8 assembly at a given incorporation percentage. The peptide inserts were modeled in the capsid environment with Monte Carlo software MCPRO (39) with solvent effects accounted for by the Poisson-Boltzmann toolkit ZAP (40).
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39
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0347571750
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W. L. Jorgensen, Yale University
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MCPRO, Version 1.68; W. L. Jorgensen, Yale University.
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MCPRO, Version 1.68
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40
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0346940835
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OpenEye Scientific Software, Cambridge, MA
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OpenEye Scientific Software, Cambridge, MA.
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41
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0347571752
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note
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We thank C. Flynn for valuable discussion. We acknowledge the use of core microscope facilities in the Texas Materials Institute at UT and the Center for Materials Science and Engineering at MIT. Supported by NSF through the Nanotechnology and Interdisciplinary Research Initiative, the Army Research Office, and the Air Force Scientific Research Office.
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