Affinity labeled glutaryl-7-amino cephalosporanic acid acylase C130 can hydrolyze the inhibitor during crystallization

Biochemical and Biophysical Research Communications

Volumn 313, Issue 3, 2004, Pages 555-558

  Zhang, Wei ^a   Huang, Xi ^a   Zhao, Guoping ^a   Jiang, Weihong ^a  

^a INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

Author keywords

BA 7 ACA; BA C130; C130; Crystallization; GL 7 ACA

Indexed keywords

7BETA BROMOACETYL AMINOCEPHALOSPORANIC ACID; ACETIC ACID; AMINO ACID DERIVATIVE; CEPHALOSPORANIC ACID DERIVATIVE; GLUTARYL 7 AMINOCEPHALOSPORANIC ACID; TRYPSIN; TRYPSIN BETA4; UNCLASSIFIED DRUG;

AFFINITY LABELING; ARTICLE; BACTERIAL STRAIN; CATALYSIS; CATALYST; CHEMICAL BOND; CHEMICAL REACTION; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; CRYSTALLIZATION; DEACYLATION; DRUG HYDROLYSIS; ENZYME ACTIVATION; ENZYME ACTIVITY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PRIORITY JOURNAL; PSEUDOMONAS; ROOM TEMPERATURE; TEMPERATURE DEPENDENCE;

PSEUDOMONAS; PSEUDOMONAS SP.; PSEUDOMONAS SP. 130;

EID: 0345802689     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2003.11.154     Document Type: Article

References (15)

1. Deshpande B.S., Ambedkar S.S., Sudhakaran V.K., Shewale J.G. Molecular biology of β-lactam acylases. World J. Microbiol. Biotechnol. 10:1994;129-138.
2. Isogai T., Ono H., Ishitani Y., Kojo H., Veda Y., Kohsaka M. Structure and expression of cDNA for D-amino acid oxidase active against cephalosporin C from Fusarium solani. J. Biochem. 108:1990;1063-1069.
3. Matsuda A., Komatsu K. Molecular cloning and structure of the gene for. 7β -(4-carboxybutanamido) cephalosporanic acid acylase from a Pseudomonas strain J. Bacteriol. 163:1985;1222-1228.
4. Aramori I., Imanaka H. Comparative characterization of a new glutaryl-7-ACA and cephalosporin C acylase. J. Ferment. Bioeng. 73:1992;185-192.
5. Ishii Y., Shimonura K. High-level production, chemical modification and site-directed mutagenesis of a cephalosporin C acylase from Pseudomonas strain N176. Eur. J. Biochem. 230:1995;773-778.
6. Saito Y., Fujimura T., Ishii Y., Noguchi Y., Miura T., Niwa M., Shimomura K. Oxidative modification of a cephalosporin C acylase from Pseudomonas strain N176 and site-directed mutagenesis of the gene. Appl. Environ. Microbiol. 62:1996;2919-2925.
7. Yang Y.L., Yun D.F., Guan Y.Q., Peng H.L., Chen J.M., He Y.S., Jiao R.S. Cloning of GL-7-ACA acylase gene from Pseudomonas sp.130 and its expression in Escherichia coli. Chin. J. Biotechnol. 7:1991;99-107.
8. Li Y., Jiang W.H., Yang Y.L., Zhao G.P., Wang E.D. Overproduction and purification of glutaryl 7-amino cephalosporanic acid acylase. Protein Expr. Purif. 12:1998;233-238.
9. Kim Y., Yoon K.H., Khang Y., Turley S., Hol W.G.J. The 2.0. Å crystal structure of cephalosporin acylase Structure. 8:2000;1059-1068.
10. Duggleby H.J., Tolley S.P., Hill C.P., Dodson E.J., Dodson G., Moody P.C. Penicillin acylase has a single-amino-acid catalytic center. Nature. 373:1995;264-268.
11. Lee Y.S., Kim H.W., Lee K.B., Park S.S. Involvement of arginine and tryptophan residues in catalytic activity of glutaryl 7-aminocephalosporanic acid acylase from Pseudomonas sp strain GK16. Biochim. Biophys. Acta. 1523:2000;123-127.
12. Timothy J.N., Ishii Y., Fujimura T., Saito Y., Niwa M. Chemical modification and site-directed mutagenesis of tyrosine residues in cephalosporin C acylase from Pseudomonas strain N176. J. Ferment. Bioeng. 77:1994;604-609.
13. Huang X., Zeng R., Ding X.M., Mao X., Ding Y., Rao Z.H., Xie Y., Jiang W.H., Zhao G.P. Affinity alkylation of the Trp-. β4 residue of the β-subunit of the glutaryl-amidocephalosporanic acid scylase of Pseudomonas sp.130 J. Biol. Chem. 277:2002;10256-10264.
14. Ichikawa S., Shibuya Y., Matsumoto K., Fujii T., Komatsu K., Kodaira R. Purification and properties of. 7β -(4-carboxybutanamido) cephalosporanic acid acylase produced by mutants derived from Pseudomonas Agric. Biol. Chem. 45:1981;2231-2236.
15. Kim Y., Hol W.G.J. Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity. Chem. Biol. 8:2001;1253-1264.