The combination of in situ hybridization and immunohistochemical analysis: An evaluation of Her2/neu expression in paraffin-embedded breast carcinomas and adjacent normal-appearing breast epithelium

Modern Pathology

Volumn 10, Issue 12, 1997, Pages 1247-1252

  Ratcliffe, Nora ^a   Wells, Wendy ^a   Wheeler, Karlya ^a   Memoli, Vincent ^a  

^a DARTMOUTH HITCHCOCK MEDICAL CENTER   (United States)

Author keywords

Breast cancer; C erbB 2; Double staining; Her2 neu; Immunohistochemistry; In situ hybridization

Indexed keywords

ONCOPROTEIN;

ARTICLE; BREAST CARCINOMA; BREAST EPITHELIUM; CONTROLLED STUDY; EMBEDDING; FLUORESCENCE IN SITU HYBRIDIZATION; GENE EXPRESSION; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; ONCOGENE NEU; PRIORITY JOURNAL;

BREAST; BREAST NEOPLASMS; BREAST NEOPLASMS, MALE; CARCINOMA, DUCTAL, BREAST; CARCINOMA, PAPILLARY; GENES, ERBB-2; HUMANS; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION, FLUORESCENCE; MALE; PARAFFIN EMBEDDING; RECEPTOR, ERBB-2; TISSUE FIXATION;

EID: 0345563219     PISSN: 08933952     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Slamon D, Clark G, Wong S, Levin W, Ullrich A, McGuire W. Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science 1987;235:177-82.
2. Schimmelpenning H, Eriksson ET, Pallis L, Skoog L, Cedermark B, Auer GU. Immunohistochemical c-erbB-2 proto-oncogene expression and nuclear DNA content in human mammary carcinoma in situ. Am J Clin Pathol 1992;97(5 Suppl 1):S48-S52.
3. Haerslev T, Krag Jacobsen G. Expression of cytokeratin and erbB-2 oncoprotein in Paget's disease of the nipple. An immunohistochemical study. APMIS 1992;100:1041-7.
4. Barnes DM. c-erbB-2 amplification in mammary carcinoma. J Cell Biochem Suppl 1993;17G:132-8.
5. Bose S, Lesser ML, Norton L, Rosen PP. Immunophenotype of intraductal carcinoma. Arch Pathol Lab Med 1996;120: 81-5.
6. Keatings L, Sinclair J, Wright C, Corbett IP, Watchorn C, Hennessy C, et al. c-erbB-2 oncoprotein expression in mammary and extramammary Paget's disease: an immunohistochemical study. Histopathology 1990;17: 243-7.
7. Vielh P, Validire P, Kheirallah S, Campana F, Fourquet A, Di Bonito L. Paget's disease of the nipple without clinically and radiologically detectable breast tumor. Histochemical and immunohistochemical study of 44 cases. Pathol Res Pract 1993;189:150-5.
8. Slamon D, Godolphin W, Jones L, Holt JA, Wong SG, Keith DE, et al. Studies of the HER-2/neu proto-oncogene in human breast and ovarian cancer. Science 1989;244:707-12.
9. Corbett IP, Angus HJ, Watchorn CJ, Wilkinson L, Hennessy C, Gullick WJ, et al. NCL-CB11: a new monoclonal antibody recognizing the internal domain of the c-erbB-2 oncogene protein effective for use on formalin-fixed, paraffin-embedded tissue. J Pathol 1990;161:15-25.
10. Singleton T, Niehans G, Gu F, Litz CE, Hagen K, Kiang DT, et al. Detection of c-erbB-2 activation in paraffin-embedded tissue by immunohistochemistry. Hum Pathol 1992;23:1141-50.
11. Han K, Hollinger F, Noonan C, Yoffe B. Simultaneous detection of HBV-specific antigens and DNA in paraffin-embedded liver tissue by immunohistochemistry and in situ hybridization using a digoxigenin-labeled probe. J Virol Meth 1992;37:89-98.
12. Ironside J, Lewis F, Blythe D, Wakefield E. The identification of cells containing JC papovavirus DNA in progressive multifocal leukoencephalopathy by combined in situ hybridization and immunocytochemistry. J Pathol 1989;157:291-7.
13. Mullink H, Walboomers M, Tadema T, Jansen D, Meijer C. Combined immuno- and non-radioactive hybridocytochemistry on cells and tissue sections: influence of fixation, enzyme pretreatment, and choice of chromogen on detection of antigen and DNA sequences. J Histochem Cytochem 1989; 37:603-9.
14. Fernandez JL, Goyanes V, Lopez-Fernandez C, Buno I, Gosalvez J. Quantification of c-erbB-2 gene amplification in breast cancer cells using fluorescence in situ hybridization and digital image analysis. Cancer Genet Cytogenet 1996;86: 18-21.
15. Kallioniemi OP, Kallioniemi A, Kurisu W, Thor A, Chen L-C, Smith HS, et al. erbB-2 amplification in breast cancer analyzed by fluorescence in situ hybridization. Proc Natl Acad Sci U S A 1992;89:5321-5.
16. Pauletti G, Godolphin W, Press MF, Slamon DJ. Detection and quantitation of Her-2/neu gene amplification in human breast cancer archival material using fluorescence in situ hybridization. Oncogene 1996;13:63-72.
17. Hollywood D, Hurst H. A novel transcription factor, OB2-1, is required for overexpression of the proto-oncogene c-erbB-2 in mammary tumour lines. EMBO J 1993;12:2369-75.
18. Natali PG, Nicotra MR, Bigotti A, Venture I, Slamon DJ, Fendly BM, et al. Expression of the p185 encoded by Her2 oncogene in normal and transformed human tissues. Int J Cancer 1990;45:457-61.
19. Press M, Cordon-Cardo C, Slamon D. Expression of the Her-2/neu proto-oncogene in normal human adult and fetal tissues. Oncogene 1990;5:953-62.
20. Deng G, Lu Y, Zlotnikov G, Thor AD, Smith HS. Loss of heterozygosity in normal tissue adjacent to breast carcinomas. Science 1996;274:2057-9.