Exhaustive identification of interaction domains using a high-throughput method based on two-hybrid screening and PCR-convergence: Molecular dissection of a kinetochore subunit Spc34p

Nucleic Acids Research

Volumn 31, Issue 23, 2003, Pages 6953-6962

  Ikeuchi, Akinori ^a   Sasaki, Yoko ^a   Kawarasaki, Yasuaki ^a,b   Yamane, Tsuneo ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; POLYPEPTIDE;

ACCURACY; ARTICLE; CENTROMERE; COMPLEX FORMATION; CONTROLLED STUDY; GENE LIBRARY; GENE MAPPING; GENETIC CONSERVATION; GENETIC SCREENING; GENOME ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; PROTEIN SECONDARY STRUCTURE; SACCHAROMYCES CEREVISIAE; TWO HYBRID SYSTEM;

AMINO ACID SEQUENCE; BINDING SITES; KINETOCHORES; MACROMOLECULAR SUBSTANCES; MICROTUBULE-ASSOCIATED PROTEINS; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; POLYMERASE CHAIN REACTION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; TWO-HYBRID SYSTEM TECHNIQUES;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0345531058     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/gkg888     Document Type: Article

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