Cloning, sequence analysis and overexpression of a Rhodothermus marinus gene encoding a thermostable thymidine kinase

FEMS Microbiology Letters

Volumn 179, Issue 2, 1999, Pages 311-316

  Blöndal, Thórarinn ^a,e   Thorbjarnardóttir, Sigrídur H ^a   Kieleczawa, Jan ^b   Einarsson, Jón M ^c   Hjörleifsdóttir, Sigrídur ^d   Kristjánsson, Jakob K ^a,c,d   Eggertsson, Gudmundur ^a  

^a UNIVERSITY OF ICELAND   (Iceland)

^b BROOKHAVEN NATIONAL LABORATORY   (United States)

^c GENIS ^*  (Iceland)

^d Technological Institute of Iceland   (Iceland)

^e DECODE GENETICS   (Iceland)

Author keywords

Rhodothermus marinus; Salvage pathway; Thermostability; Thymidine kinase

Indexed keywords

THYMIDINE KINASE;

AMINO ACID SEQUENCE; ARTICLE; GENE OVEREXPRESSION; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; THERMOPHILIC BACTERIUM; THERMOSTABILITY;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; GENES, BACTERIAL; GRAM-NEGATIVE BACTERIA; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; RECOMBINANT PROTEINS; THYMIDINE KINASE;

BACTERIA (MICROORGANISMS); EUKARYOTA; PROKARYOTA; RHODOTHERMUS MARINUS;

EID: 0345425632     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(99)00428-0     Document Type: Article

References (22)

1. Alfredsson, G.A., Kristjánsson, J.K., Hjörleifsdóttir, S. and Stetter, K.O. (1988) Rhodothermus marinus, gen. Nov., sp. Nov., a thermophilic halophilic bacterium from submarine hot springs in Iceland. J. Gen. Microbiol. 134, 299-306.
2. Andrésson, Ó.S. and Fridjónsson, Ó.H. (1994) The sequence of the single 16S rRNA gene of the thermophilic eubacterium Rhodothermus marinus reveals a distant relationship to the group containing Flexibacter, Bacteroides and Cytophaga species. J. Bacteriol. 176, 6165-6169.
3. Black, M.E. and Hruby, D.E. (1991) Nucleotide sequence of the Escherichia coli thymidine kinase gene provides evidence for conservation of functional domains and quaternary structure. Mol. Microbiol. 5, 373-379.
4. McNab, R. (1996) Cloning and sequence of thymidine kinase from the oral bacterium Streptococcus gordonii. FEMS Microbiol. Lett. 135, 103-110.
5. Franke, C.A. and Hruby, D.E. (1993) Expression and single step purification of enzymatically active vaccinia virus thymidine kinase containing an engineered oligohistidine domain by immobilized metal affinity chromatography. Protein Expr. Purif. 4, 101-109.
6. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
7. Chang, A.C.J. and Cohen, S.N. (1978) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the Pl5A cryptic miniplasmid. J. Bacteriol. 134, 1141-1156.
8. Thorbjarnardóttir, S.H., Jónsson, Z.O., Andrésson, Ó.S., Kristjánsson, J.K., Eggertsson, G. and Pálsdóttir, Á. (1995) Cloning and sequence analysis of the DNA ligase-encoding gene of Rhodothermus marinus, and overproduction, purification and characterization of two thermophilic DNA ligases. Gene 161, 1-6.
9. Degryse, E., Glansdorff, N. and Piérard, A. (1978) A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. Arch. Microbiol. 117, 189-196.
10. Ausubel, F.M., Brent, R., Kingston, R.E., More, D.D., Seidman, J.G., Smith, J.A. and Strahl, K. (1989) Current Protocols in Molecular Biology, Vol. I. Greene Publishing Associates and Wiley-Interscience, New York.
11. Dunn, J.J., Butler-Loffredo, L.L. and Studier, W.F. (1995) Ligation of hexamers on hexamer templates to produce primers for cycle sequencing of the polymerase chain reaction. Anal. Biochem. 228, 99-100.
12. Altschul, S.F., Gish, W., Miller, W., Myers, E.W. and Lipman, D.J. (1990) Basic local alignment search tool. J. Mol. Biol. 215, 403-410.
13. Hames, B.D. and Rickwood, D. (1990) Gel Electrophoresis of Proteins. The Practical Approch Series. IRL Press, Oxford.
14. Bradford, M.M. (1979) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
15. Hruby, D.E., Maki, R.A., Miller, D.B. and Ball, L.A. (1983) Fine structure analysis and nucleotide sequence of the vaccinia virus thymidine kinase gene. Proc. Natl. Acad. Sci. USA 80, 3411-3415.
16. Quirk, P.G., Dunkley Jr., E.A., Lee, P. and Krulwich, T.A. (1993) Identification of a putative Bacillus subtilis rho gene. J. Bacteriol. 175, 647-654.
17. Himmelreich, R., Hilbert, H., Plagens, H., Pirkl, E., Li, B.C. and Herrmann, R. (1996) Complete sequence analysis of the genome of the bacterium Mycoplasma pneumonia. Nucleic Acids Res. 24, 4420-4449.
18. Vieille, C. and Zeikus, G. (1996) Thermozymes: Identifying molecular determinants of protein structural and functional stability. Trends Biotechnol. 14, 183-190.
19. Hruby, D.E. (1985) Inhibition of vaccinia virus thymidine kinase by the distal protucts of its own metabolic pathway. Virus Res. 2, 151-156.
20. Black, M.E. and Hruby, D.E. (1990) Identification of the ATP-binding domain of vaccinia virus thymidine kinase. J. Biol. Chem. 265, 17584-17592.
21. Danson, M.J., Hough, D.V., Russel, R.M.J., Taylor, G.L. and Pearl, L. (1996) Enzyme thermostability and thermoactivity. Protein Eng. 9, 629-630.
22. Macedo-Ribeiro, S., Darimont, B., Sterner, R. and Huber, R. (1996) Small structural changes account for the high thermostability of l[4Fe-4S] ferredoxin from hyperthermophilic bacterium Thermotoga maritima. Structure 4, 1291-1301.