Cloning, characterization and serological evaluation of K9 and K26: Two related hydrophilic antigens of Leishmania chagasi

Molecular and Biochemical Parasitology

Volumn 102, Issue 2, 1999, Pages

  Bhatia, Ajay ^a,b   Daifalla, Nada S ^b,c   Jen, Shyian ^a   Badaro, Roberto ^d   Reed, Steven G ^a,b,e   Skeiky, Yasir A W ^a  

^a GLAXOSMITHKLINE   (United States)

^b INFECTIOUS DISEASE RESEARCH INSTITUTE   (United States)

^c UNIVERSITY OF KHARTOUM   (Sudan)

^d FEDERAL UNIVERSITY OF BAHIA   (Brazil)

^e UNIVERSITY OF WASHINGTON   (United States)

Author keywords

K26; K9; Leishmania chagasi; Serodiagnostics; Visceral leishmaniasis

Indexed keywords

AMASTIGOTE; AMINO ACID SEQUENCE; ANTIGEN EXPRESSION; ANTIGEN PURIFICATION; ARTICLE; HUMAN; HUMAN TISSUE; IMMUNOBLOTTING; LEISHMANIA CHAGASI; LEISHMANIA DONOVANI; LEISHMANIASIS; LIFE CYCLE; MOLECULAR CLONING; MOLECULAR WEIGHT; OPEN READING FRAME; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROMASTIGOTE; PROTEIN ANALYSIS; SEQUENCE HOMOLOGY; SERODIAGNOSIS;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, PROTOZOAN; BASE SEQUENCE; BLOTTING, SOUTHERN; CLONING, MOLECULAR; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENES, PROTOZOAN; HUMANS; LEISHMANIA; LEISHMANIA MAJOR; LEISHMANIASIS, VISCERAL; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES; RABBITS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA;

LEISHMANIA DONOVANI; LEISHMANIA DONOVANI CHAGASI; NASO HEXACANTHUS;

EID: 0345286871     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(99)00098-5     Document Type: Article

References (29)

1. Burns J.M., Shreffler W.G., Benson D.R., Ghalib H.W., Badaro R., Reed S.G. Molecular characterization of a kinesin-related antigen of Leishmania chagasi that detects specific antibody in African and American visceral leishmaniasis. Proc. Natl. Acad. Sci. USA. 90:1993;775-779.
2. Lee M.B., Gilbert H.M. Current approaches to leishmaniasis. Infect. Med. 16(1):1999;34-45.
3. Badaro R., Benson D., Eulalio M.C. et al. rK39: A cloned antigen of Leishmania chagasi that predicts active visceral leishmaniasis. J. Infect. Dis. 173:1996;758-761.
4. Qu J., Zhong L., Masoom-Yasinzai M., Abdur-Rab M., Aksu H.S.Z., Reed S.G., Chang K.P. Serodiagnosis of asian leishmaniasis with a recombinant antigen from the repetitive domain of a Leishmania kinesin. Trans. R. Soc. Trop. Med. Hyg. 88:1994;543-545.
5. Flinn H.M., Rangarajan D., Smith D.F. Expression of a hydrophilic surface protein in infective stages of Leishmania major. Mol. Biochem. Parasitol. 65:1994;259-270.
6. Feinberg A.P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132:1983;6-13.
7. Chomczynski P., Sacchi N. Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:1987;156-159.
8. Webb J.R., Vedvick T.S., Alderson M.R. et al. Molecular cloning, expression and immunity of MTB12, a novel low molecular-weight antigen secreted by Mycobacterium tuberculosis. Infect. Immun. 66:1998;4208-4214.
9. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354.
10. McKean P.G., Delahay R., Pimenta P.F.P., Smith D.F. Characterization of a second protein encoded by differentially regulated LmcDNA16 gene family of Leishmania major. Mol. Biochem. Parasitol. 85:1997;221-231.
11. McKean P.G., Trenholme K.R., Rangarajan D., Keen J.K., Smith D.F. Diversity in the repeat-containing surface proteins of Leishmania major. Mol. Biochem. Parasitol. 86:1997;225-235.
12. Schnur L.F., Jacobson R.L. Surface reaction of Leishmania IV. Variation in the surface membrane carbohydrates of different strains of Leishmania major. Ann. Trop. Med. Parasitol. 83:1989;455-463.
13. Kemp D.J., Coppel R.L., Anders R.F. Repetitive proteins and genes of Malaria. Ann. Rev. Microbiol. 41:1987;181-208.
14. Anders R.F. Multiple cross-reactivities amongst antigens of Plasmodium falciparum impair the development of protective immunity against malaria. Parasite Immunol. 8:1986;529-539.
15. Zavala F., Cochrane A.H., Nardin E.H., Nussenzweig R.S., Nussenzweig V. Circumsporozoite proteins of malaria parasites contain a single immunodominant region with two or more identical epitopes. J. Exp. Med. 157:1983;1947-1957.
16. Godson G.N., Ellis J., Svec P., Schlesinger D.H., Nussenzweig V. Identification and chemical synthesis of a tandemly repeated immunogenic region of Plasmodium knowlesi circumsporozoite protein. Nature. 305:1983;29-33.
17. Kemp DJ, Coppel RL, Stahl HD, Bianco AE, Corcoran LM, McIntyre P, Langford CJ, Favaloro JM, Crewther PE, Brown GV, Mitchell GF, Culvenor JG, Anders RF. Genes for antigens of Plasmodium falciparum. Parasitology 1986;91 Suppl 83-108.
18. Stahl H.D., Coppel R.L., Brown G.V., Saint R.B., Lingelbach K. et al. Differential antibody screening of cloned Plasmodium falciparum sequences expressed in Escherichia coli: procedure for isolation of defined antigens and analysis of human antisera. Proc. Natl. Acad. Sci. USA. 81:1984;2456-2460.
19. Rodriguez C., Afchain D., Capron M., Dissous C., Santoro F. Major surface protein of Toxoplasma gondii (p30) contains an immunodominant region with repetitive epitopes. Eur. J. Immunol. 15:1985;747-749.
20. Peterson D.S., Wrightsman R.A., Manning J.E. Cloning of a major surface-antigen gene of Trypanosoma cruzi and identification of a nonapeptide repeat. Nature. 322:1986;566-568.
21. Ibanez C.F., Affranchino J.L., Macina R.A., Reyes M.B., Leguizamon S., Camargo M.E., Aslund L., Pettersson U., Frasch A.C.C. Multiple Trypanosoma cruzi antigens containing tandemly repeated amino acid sequence motifs. Mol. Biochem. Parasitol. 30:1988;27-34.
22. Lafaille J.J., Linss J., Krieger M.A., Souto-Padron T., de Souzza W., Goldenberg S. Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes. Mol. Biochem. Parasitol. 35:1989;127-136.
23. Wallis A.E., McMaster W.R. Identification of Leishmania genes encoding proteins containing tandemly repeating peptides. J. Exp. Med. 166:1987;1814-1824.
24. Flinn H.M., Smith D.F. Genomic organization and expression of a differentially regulated gene family from Leishmania major. Nucl. Acids Res. 20:1992;755-762.
25. Aly R., Argaman M., Halman D., Shapira M. A regulatory role for 5′ and 3′ untranslated regions in differential expression of hsp83 in Leishmania. Nucl. Acids Res. 22:1994;2922-2929.
26. Wilson M.E., Paetz K.E., Ramamoorthy R., Donelson J.E. The effect of ongoing protein synthesis on the steady state levels of GP63 RNAs in Leishmania chagasi. J. Biol. Chem. 268:1993;15731-15736.
27. Li S., Wilson M.E., Donelson J.E. A gene encoding a protein kinase with a catalytic domain structurally related to MAP kinase kinase. Exp. Parasitol. 82:1996;87-96.
28. Jensen A.T.R., Gaafar A., Ismail C., Christensen C.B.V., Kemp M., El Hassan A.M., Kharazmi A., Theander T.G. Serodiagnosis of cutaneous leishmaniasis: Assessment of an enzyme-linked immunosorbent assay using a peptide sequence from Gene B protein. Am. J. Trop. Med. Hyg. 55:1996;490-495.
29. Sundar S., Reed S.G., Singh V.P., Kumar P.C.K., Murray H.W. Rapid accurate field diagnosis of Indian visceral leishmaniasis. Lancet. 351:1998;563-565.