Enzymatic specificity and hydrolysis pattern of the catalytic domain of the xylanase Xyn1 from Rhodothermus marinus

Journal of Biotechnology

Volumn 60, Issue 1-2, 1998, Pages

  Karlsson, Eva Nordberg ^a   Dahlberg, Leif ^a   Torto, Nelson ^a,b   Gorton, Lo ^a   Holst, Olle ^a  

^a LUND UNIVERSITY   (Sweden)

^b UNIVERSITY OF BOTSWANA   (Botswana)

Author keywords

Glycosyl hydrolase family 10; Rhodothermus marinus; Xylanase

Indexed keywords

AMINO ACIDS; CATALYST ACTIVITY; HYDROLYSIS; OLIGOMERS; PH; POLYSACCHARIDES;

ENZYMATIC SPECIFICITY; XYLANASE;

ENZYME KINETICS;

XYLAN ENDO 1,3 BETA XYLOSIDASE;

AMINO ACID SEQUENCE; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CATALYSIS; ENZYME ASSAY; ENZYME SPECIFICITY; ESCHERICHIA COLI; HYDROLYSIS; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DOMAIN; TEMPERATURE; THERMOPHILIC BACTERIUM;

AMINO ACID SEQUENCE; BASE SEQUENCE; CATALYSIS; ESCHERICHIA COLI; GRAM-NEGATIVE AEROBIC BACTERIA; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; ISOELECTRIC POINT; KINETICS; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; XYLAN ENDO-1,3-BETA-XYLOSIDASE; XYLOSIDASES;

ESCHERICHIA COLI; RHODOTHERMUS MARINUS; TRITICUM AESTIVUM SUBSP. SPELTA;

EID: 0038687875     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(97)00178-8     Document Type: Article

References (31)

1. Alfredsson, G.A., Kristjansson, J.K., Hjörleifsdottir, S., Stetter, K.O., 1988. Rhodothermus marinus, gen. nov., sp. nov., a thermophilic bacterium from submarine hot springs in Iceland. J. Gen. Microbiol. 134, 299-306.
2. Andrésson, O.S., Fridjónsson, O.H., 1994. The sequence of the single 16S rRNA gene of the thermophilic eubacterium Rhodothermus marinus reveals a distant relationship to the group containing Flexibacter, Bacteroides and Cytophaga species. J. Bacteriol. 176, 6165-6169.
3. Bailey, M.J., Biely, P., Poutanen, K., 1992. Interlaboratory testing of methods for assay of xylanase activity. J. Biotechnol. 23, 257-270.
4. Biely, P., Vrsanska, M., Kucar, S., 1992. Identification and mode of action of endo-(1 - > 4)-β-xylanases. In: Visser, J. (Ed.), Xylans and Xylanases. Elsevier, Amsterdam, pp. 81-95.
5. Dahlberg, L., Holst, O., Kristjansson, J.K., 1993. Thermostable xylanolytic enzymes from Rhodothermus marinus grown on xylan. Appl. Microbiol. Biotechnol. 40, 63-68.
6. Gray, C.J., 1993. Stabilisation of enzymes with soluble additives. In: Gupta, M.N. (Ed.), Thermostability of Enzymes. Narosa, New Delhi, pp. 124-143.
7. Grépinet, O., Chebrou, M.-C., Béguin, P., 1988. Nucleotide sequence and deletion analysis of the xylanase gene (xynZ) of Clostridium thermocellum. J. Bacteriol. 170, 4582-4588.
8. Harris, G.W., Jenkins, J.A., Connerton, I., Cummings, N., Lo Leggio, L., Scott, M., Hazlewood, G.P., Laurie, J.I., Gilgert, H.J., Pickersgill, R.W., 1994. Structure of the catalytic core of the family F xylanase from Pseudomonas fluorescens and identification of the xylopentaose-binding sites. Structure 2, 1107-1116.
9. Henrissat, B., 1990. A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 280, 309-316.
10. Herbers, K., Wilke, I., Sonnewald, U., 1995. A thermostable xylanase from Clostridium thermocellum expressed at high levels in the apoplast of transgenic tobacco has no detrimental effects and is easily purified. Bio/Technology 13, 63-66.
11. Kellett, L.E., Poole, D.M., Ferreira, L.M.A., Durrant, A.J., Hazlewood, G.P., Gilbert, H.J., 1990. Xylanase B and an arabinofuranosidase from Pseudomonas fluorescens subsp. cellulosa contain identical cellulose-binding domains and are encoded by adjacent genes. Biochem. J. 272, 369-376.
12. Kristjansson, J.K., 1989. Thermophilic organisms as sources of thermostable enzymes. Trends Biotechnol. 7, 349-353.
13. Laemmli, U., 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227, 680-685.
14. Lee, Y.-E., Lowe, S.E., Henrissat, B., Zeikus, J.G., 1993a. Characterization of the active site and thermostability regions of endoxylanase from Thermoanaerobacterium saccharolyticum B6A-RI. J. Bacteriol. 175, 5890-5898.
15. Lee, Y.-E., Lowe, S.E., Zeikus, J.G., 1993b. Gene cloning, sequencing and biochemical characterization of endoxylanase from Thermoanaerobacterium saccharolyticum B6A-RI. Appl. Environ. Microbiol. 59, 3134-3137.
16. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
17. Manelius, A., Dahlberg, L., Holst, O., 1994. Some properties of a thermostable β-xylosidase from Rhodothermus marinus. Appl. Biochem. Biotechnol. 44, 39-48.
18. Miller, G.L., Blum, R., Glennon, W.E., Burton, A.L., 1960. Measurement of carboxymethylcellulase activity. Anal. Biochem. 2, 127-132.
19. Nordberg Karlsson, E., Bartonek-Roxå, E., Holst, O., 1997. Cloning and sequence of a thermostable multidomain xylanase from the bacterium Rhodothermus marinus. Biochim. Biophys. Acta 1353, 118-124.
20. Nordberg Karlsson, E., Linå Jørgensen, P., Dahlberg, L., Holst, O., 1996. Cloning of xylanolytic activities from Rhodothermus marinus. In: Srebotnik, E., Messner, K. (Eds.), Biotechnology in the Pulp and Paper Industry. Recent Advances in Applied and Fundamental Research. WUV-Universitätsverlag, Vienna, pp. 513-516.
21. Nunes, O., Donato, M.M., Da Costa, M.S., 1992. Isolation and characterization of Rhodothermus strains from S. Miguel, Azores. Syst. Appl. Microbiol. 15, 92-97.
22. Paice, M., Gurnagul, N., Page, D., Jurasek, L., 1992. Mechanism of hemicellulose-directed prebleaching of kraft pulps. Enzyme Microb. Technol. 14, 272-276.
23. Paradis, F.W., Zhu, H., Krell, P.J., Phillips, J.P., Forsberg, C.W., 1993. The xynC gene from Fibrobacter succinogenes S85 codes for a xylanase with two similar catalytic domains. J. Bacteriol. 175, 7666-7672.
24. Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual, 2nd. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
25. Tenkanen, M., Siika-aho, M., Hausalo, T., Puls, J., Viikari, L., 1996. Synergism of xylanolytic enzymes of Trichoderma reesei in the degradation of acetyl-4-o-methylglucuronoxylan. In: Srebotnik, E., Messner, K. (Eds.), Biotechnology in the Pulp and Paper industry. Recent Advances in Applied and Fundamental Research. WUV-Universitätsverlag, Vienna, pp. 503-508.
26. Torto, N., Buttler, T., Gorton, L., Marko-Varga, G., Stålbrand, H., Tjerneld, F., 1995. Monitoring of enzymatic hydrolysis of ivory nut mannan using on-line microdialysis sampling and anion-exchange chromatography with integrated pulsed electrochemical detection. Anal. Chim. Acta 313, 15-24.
27. Winterhalter, C., Heinrich, P., Candussio, A., Wich, G., Liebl, W., 1995. Identification of a novel cellulose-binding domain within the multidomain 120 kDa xylanase Xyn A of the hyperthermophilic bacterium Thermotoga maritima. Mol. Microbiol. 15, 431-444.
28. Winterhalter, C., Liebl, W., 1995. Two extremely thermostable xylanases of the hyperthermophilic bacterium Thermotoga maritima MSB8. Appl. Environ. Microbiol. 61, 1810-1815.
29. Wong, K.K.Y., Saddler, J.N., 1993. Applications of hemicellulases in the food, feed, and pulp and paper industries. In: Coughlan, M.P., Hazlewood, G.P. (Eds.), Hemicellulose and Hemicellulases. Portland Press, London, pp. 127-143.
30. Wong, K.K.Y., Tan, L.U.L., Saddler, J.N., 1988. Multiplicity of β-,4-xylanase in microorganisms: Functions and applications. Microbiol. Rev. 52, 305-317.
31. Zhu, H., Paradis, F.W., Krell, P.J., Phillips, J.P., Forsberg, C.W., 1994. Enzymatic specificities and modes of action of the two catalytic domains of the xynC xylanase from Fibrobacter succinogenes S85. J. Bacteriol. 176, 3885-3894.