Changes in the nutrient composition of Tetraselmis suecica cultured semicontinuously with different nutrient concentrations and renewal rates

Aquaculture

Volumn 159, Issue 1-2, 1997, Pages 111-123

  Otero, Ana ^a   Fábregas, Jaime ^a  

^a UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

Author keywords

Biochemical composition; Carbohydrates; Continuous culture; Fatty acids; Lipid; Protein; Tetraselmis suecica

Indexed keywords

TETRASELMIS SUECICA;

EID: 0345084481     PISSN: 00448486     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0044-8486(97)00214-7     Document Type: Article

References (41)

1. Albentosa, M., Labarta, U., Fernandez-Reiriz, M.J., Perez Camacho, A., 1996. Fatty acid composition of Ruditapes deccussatus spat fed on different microalgae diets. Comp. Biochem. Physiol. 113(A), 113-119.
2. Berges, J.A., Fisher, A.E., Harrison, P.J., 1993. A comparison of Lowry, Bradford and Smith protein assays using different protein standards and protein isolated from the marine diatom Thalassiosira pseudonana. Mar. Biol. 115, 187-193.
3. Bligh, E.G., Dyer, W.J., 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911-917.
4. Borowitzka, M.A., 1988. Fats, oils and hydrocarbons. In: Borowitzka, M.A., Borowitzka, L.J. (Eds.), Microalgal Biotechnology. Cambridge Univ. Press, London, pp. 257-287.
5. Brown, M.R., Garland, C.D., Jeffrey, S.W., Jameson, I.D., LeRoi, J.M., 1993. The gross and amino acid compositions of batch and semicontinuous cultures of Isochrysis sp. (clone T-Iso), Pavlova lutheri and Nannochloropsis oculata. J. Appl. Phycol. 5, 285-296.
6. Davidson, K., Flynn, K.J., Cunningham, A., 1991. Relationships between photopigment, cell carbon, cell nitrogen and growth rate for a marine nanoflagellate. J. Exp. Mar. Biol. Ecol. 153, 87-96.
7. Droop, M.R., 1983. 25 years of algal growth kinetics: a personal view. Botanica Marina 26, 99-112.
8. Fábregas, J., Arán, J., Morales, E.D., Lamela, T., Otero, A., 1997. Modification of the sterol composition in marine microalgae. Phytochemistry. In press.
9. Fábregas, J., Herrero, C., Cabezas, B., Abalde, J., 1985. Mass culture and biochemical variability of the marine microalga Tetraselmis suecica Kylin (Butch) with high nutrient concentrations. Aquaculture 49, 231-244.
10. Fábregas, J., Patiño, M., Arredondo-Vega, B.O., Tobar, J.L., Otero, A., 1995a. Renewal rate and nutrient concentration as tools to modify productivity and biochemical composition of cyclostat cultures of the marine microalga Dunaliella tertiolecta. Appl. Microbiol. Biotechnol. 44, 287-292.
11. Fábregas, J., Patiño, M., Vecino, E., Cházaro, F., Otero, A., 1995b. Productivity and biochemical composition of cyclostat cultures of the marine microalga Tetraselmis suecica. Appl. Microbiol. Biotechnol. 43, 617-621.
12. Fábregas, J., Cid, A., Morales, E., Cordero, B., Otero, A., 1996a. Discrepancies between cell volume and organic content in semi-continuous cultures of a marine microalga. Lett. Appl. Microbiol. 22, 206-208.
13. Fábregas, J., Patiño, M., Morales, E., Cordero, B., Otero, A., 1996b. Optimal renewal rate and nutrient concentration for the production of the marine microalga Phaeodactylum tricornutum in semicontinuous cultures. Appl. Environ. Microbiol. 62, 266-268.
14. Fábregas, J., Patiño, M., Morales, E.D., Dominguez, A., Otero, A., 1996c. Distinctive control of metabolic pathways by Chlorella autotrophica in semicontinuous cultures . Can. J. Microbiol. 42, 1087-1090.
15. Fernández-Reiriz, M.J., Perez-Camacho, A., Ferreiro, M.J., Blanco, J., Planas, M., Campos, M.J., Labarta, U., 1989. Biomass production and variation in the biochemical profile (total protein, carbohydrates, RNA, lipids and fatty acids) of seven species of marine microalgae. Aquaculture 83, 17-37.
16. Gnaiger, E., Bitterlich, G., 1984. Proximate biochemical composition and caloric content calculated from elemental CHN analysis: a stoichiometric concept. Oecologia 62, 289-298.
17. 2 in higher plants, algae and natural populations. Biochem. Physiol. Pflanz. 167, 191-194.
18. Kochert, G., 1978. Carbohydrate determination by the phenol-sulfuric acid method. In: Hellebust, J.A., Craigie, J.S. (Eds.), Handbook of Phycological Methods. Physiological and Biochemical Methods. Cambridge Univ. Press, London, pp. 95-97.
19. Laing, I., Jones, E., 1988. A turbidostat vessel for the continuous culture of marine microalgae. Aquacult. Eng. 7, 89-96.
20. Laws, E.A., Bannister, T.T., 1980. Nutrient and light limited growth of Thalassiosira fluviatilis in continuous culture, with implications for phytoplankton growth in the ocean. Limnol. Oceanogr. 25, 457-473.
21. Lowry, O.H., Rosebrough, H.J., Farr, A.L., Randall, R.J., 1951. Protein measurement with the Folin-phenol reagent. J. Biol. Chem. 193, 265-275.
22. Marsh, J.B., Weinstein, D.B., 1966. Simple charring method for determination of lipids. J. Lipid Res. 7, 574-576.
23. National Research Council, 1977. Nutrient requirements of warmwater fishes. Nat. Acad. Press, Washington, DC.
24. Otero, A., 1995. Modificación de la composición bioquimica de microalgas marinas en régimen de ciclostato. PhD thesis, University of Santiago, Santiago, Spain.
25. Otero, A., Arredondo-Vega, B.O., Patiño, M., Lamela, T., Fábregas, J., 1995. Production of eicosapentaenoic and docosahexaenoic acids in semicontinuous cultures of the marine diatom Phaeodactylum tricornutum. J. Mar. Biotechnol. 3, 82-85.
26. Otero, A., García, D., Morales, E.D., Arán, J., Fábregas, J., 1997. Manipulation of the biochemical composition of the eicosapentaenoic acid-rich microalga Isochrysis galbana in semicontinuous cultures. Biotechnol. Appl. Biochem. In press.
27. Reitan, K.I., Rainuzzo, J.R., Olsen, Y., 1994. Effect of nutrient limitation on fatty acid and lipid content of marine microalgae. J. Phycol. 30, 972-979.
28. Richmond, A., Boussiba, S., Vonshak, A., Kopel, R., 1993. A new tubular reactor for mass production of microalgae outdoors. J. Appl. Phycol. 5, 327-332.
29. Sato, N., Murata, N., 1988. Membrane lipids. Meth. Enzymol. 167, 251-259.
30. Scott, J.M., 1980. Effect of growth rate of the food alga on the growth/ingestion efficiency of a marine herbivore. J. Mar. Biol. Assoc. U.K. 60, 681-702.
31. Spectorova, L.V., Goronkova, O.I., Nosova, L.P., Albitskaya, O.N., 1981/1982. High density culture of marine microalgae - promising items for mariculture: I. Mineral feeding regime and installations for culture of Dunaliella tertiolecta Butch. Aquaculture 26, 289-302.
32. Sukenik, A., Wahnon, R., 1991. Biochemical quality of marine unicellular algae with special emphasis on lipid composition: I. Isochrysis galbana. Aquaculture 97, 61-72.
33. Sukenik, A., Zmora, O., Carmeli, Y., 1993. Biochemical quality of marine unicellular algae with special emphasis on lipid composition: II. Nannochloropsis sp. Aquaculture 117, 313-326.
34. Taub, F.B., 1980. Use of continuous culture technique to control nutritional quality. In: Shelef, G., Soeder, C.J. (Eds.), Algae Biomass. Production and Use. Elsevier/North Holland Biomedical Press, Amsterdam, pp. 707-721.
35. Thomas, W.H., Seibert, D.L.R., Alden, M., Neori, A., Eldridge, P., 1984. Yields, photosinthetic efficiencies and proximate composition of dense marine microalgal cultures: II. Dunaliella primolecta and Tetraselmis suecica experiments. Biomass 5, 211-225.
36. Wangersky, P.J., Parrish, C.C., Wangersky, C.P., 1989. An automated mass culture system for phytoplankton. J. Shellfish Res. 8, 249-252.
37. Watanabe, T., Kitajima, C., Fujita, S., 1983. Nutritional values of live food organisms used in Japan for mass propagation of fish: a review. Aquaculture 34, 115-143.
38. Whyte, J.N.C., 1987. Biochemical composition and energy content of six species of phytoplankton used in mariculture of bivalves. Aquaculture 60, 231-241.
39. Wikfors, G.H., 1986. Altering growth and gross chemical composition of two microalgal molluscan food species by variying nitrate and phosphate. Aquaculture 59, 1-14.
40. Wikfors, G.H., Patterson, G.W., Ghosh, P., Lewin, R.A., Smith, B.C., Alix, J.H., 1996. Growth of post-set oysters, Crassostrea virginica, on high-lipid strains of algal flagellates Tetraselmis spp. Aquaculture 143. 411-419.
41. Wilhelm, C., Manns, L., 1991. Changes in pigmentation of phytoplankton species during growth and stationary phase-consequences for reliability of pigment based methods of hiomass determination. J. Appl. Phycol. 3, 305-310.