Optimal renewal rate and nutrient concentration for the production of the marine microalga Phaeodactylum tricomutum in semicontinuous cultures

Applied and Environmental Microbiology

Volumn 62, Issue 1, 1996, Pages 266-268

  Fábregas, Jaime ^a   Patiño, Manuel ^a   Morales, Ever D ^a   Cordero, Beatriz ^a   Otero, Ana ^a  

^a UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ALGA; ARTICLE; BIOENGINEERING; CELL DENSITY; MARINE ENVIRONMENT; NONHUMAN; PROTEIN CONTENT;

PHAEODACTYLUM TRICORNUTUM;

EID: 0030049031     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.1.266-268.1996     Document Type: Article

References (21)

1. Arao, T., A. Kawaguchi, and M. Yaniada. 1987. Positional dislrihulion of fatty acids in lipids of the marine diatom Phaeodactylum tricornutum. Phylochemistry 26:2573-2576.
2. Chrismadha, T., and M. Borowitzka. 1994. Effect of cell density and irradiance on growth, proximate composition and eicosapentaenoic acid production of Phaeodactylum tricomutum grown in a tubular photobioreactor. J. Appl. Phycol. 6:67-74.
3. Fábregas, J., A. Cid, E. Morales, B. Cordera, and A. Otero. Discrepancies between cell volume and organic content in semi-continuous cultures of a marine microalga. Lett. Appl. Microbiol., in press.
4. Fábregas, J., M. Patiño, B. O. Arredondo-Vega, J. L. Tobar, and A. Otero. Renewal rate and nutrient concentration as tools to modify productivity and biochemical composition of cyclostat cultures of the marine microalga Dunaliella teniolecta. Appl. Microbiol. Biotechnol., in press.
5. Fábregas, J., M. Patiño, E. Vecino, F. Cházaro, and A. Otero. 1995. Effect of nutrient concentration on the productivity and biochemical composition of cyclostat cultures of the marine microalga Tetraselmis suecica. Appl. Microbiol. Biotechnol. 45:617-621.
6. Geider, R. J., B. A. Osborne, and J. A. Raven. 1985. Light dependence of growth and photosynthesis in Phaeodactylum tricomutum. J. Phycol. 21:609-619,
7. Gnaiger, E., and G. Bitterlich. 1984. Proximate biochemical composition and caloric content calculated from elemental CHN analysis: a stoichiometric concept. Oecologia 62:289-298.
8. Goldman, J. C., and D. J. Peavey. 1979. Steady-state growth and chemical composition of the marine chlorophyte Dunaliella tertiolecta in nitrogen-limited continuous cultures. Appl. Environ. Microbiol. 38:894-901.
9. Kaixian, Q., and M. A. Borowitzka. 1993. Light and nitrogen deficiency effects on the growth and composition of Phaeodaclylum tricornutum. Appl. Biochem. Biotech. 38:93-103.
10. Kochert, G. 1978. Carbohydrate determination by the phenol-sulfuric acid method, p. 95-97. In J. A. Hellebust and J. S. Craigie (ed.). Handbook of phycological methods: physiological and biochemical methods. Cambridge University Press, Cambridge.
11. Larson, T. R., and T. A. V. Rees. 1994. Arrest of cell division but not protein synthesis in sodium-deficient cells of the marine diatom Phaeodactylum tricorrutum. Planta 195:195-200.
12. Laws, E. A., D. R. Jones, K. L. Terry, and J. Hirata. 1985. Modifications in recent models of phytoplankton growth: theoretical developments and experimental examination of predictions. J. Theor. Biol. 114:323-341.
13. Marsh, J. B., and D. B. Weinstein. 1966. Simple charring method for determination of lipids. J. Lipid Res 7:574-576.
14. Marsol, P., A. D. Cemhella, and L. Houle. 1991. Growth kinetics and nitrogen nutrition of the marine diatom Phaeodactylum tricornutum in continuous dialysis culture. J. Appl. Phycol. 3:1-10.
15. Molina Grima, E., F. García Camacho, J. A. Sánchez Pérez, J. Urda Cardona, F. G. Acién Fernandez, and J. M. Fernández Sevilla. 1994. Outdoor chemostat culture of Ptiarodtictylum tricornutum UTEX 640 in a tubular photobioreaclor for the production of eicosapentaenoic acid. Biotechnol. Appl. Biochem. 20:279-290.
16. Otero, A., B. O. Arredondo-Vega, M. Patiño, M., T. Lamela, and J. Fáhregas. Production of eicosapentaenoic and docosahesaenoic acids in semi-continuous cultures of the marine diatom Phaeodactylum tricomutum. J. Mar. Biotechnol., in press.
17. Parrish, C. C., and P. J. Wangersky. 1987. Paniculate and dissolved lipid classes in cultures of Phaeodactylum tricomuium grown in cage culture turbidostats with a range of nitrogen supply rates. Mar. Ecol. Prog. Ser. 35:119-128.
18. Terry, K. L., J. Hirata, and E. A. Laws. 1985. Light-, nitrogen-, and phosphorus-limited growth of Phaceodactylum tricoruntum Bohlin strain TFX-1: chemical composition, carbon partitioning, and the diel periodicity of physiological processes. J. Exp. Mar. Biol. Ecol. 86:85-100.
19. Thomas, W. H., D. L. R. Siebert, M. Alden, A. Neroi, and P. Eldridge. 1984. Yields, photosynthetic efficiencies and proximate composition of dense marine microalgal cultures. I. Introduction and Phaeodactylum tricomutum experiments. Biomass 5:181-209.
20. Yongmanitchai, W., and O. P. Ward. 1991. Screening of algae for potential alternative sources of eicosapentaenoic acid. Phytochemistry 30:2963-2967.
21. Yongmanitchai, W., and O. P. Ward. 1992. Growth and eicosapentaenoic acid production by Phaeodactylum tricomulum in batch and continuous culture systems. J. Am. Oil Chem. Soc. 69:584-590.