Nonglucosylated oligosaccharides are transferred to protein in MI8-5 Chinese hamster ovary cells

Glycobiology

Volumn 9, Issue 1, 1999, Pages 65-72

  Quellhorst Jr , George J ^b,c   O'Rear, Jessica L ^b   Cacan, René ^a   Verbert, André ^a   Krag, Sharon S ^b  

^a UNIVERSITÉ DES SCIENCES ET TECHNOLOGIES DE LILLE   (France)

^b JOHNS HOPKINS BLOOMBERG SCHOOL OF PUBLIC HEALTH   (United States)

^c HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

Author keywords

ALG6; CHO cells; Glucosyl transferase; N linked glycosylation

Indexed keywords

ALPHA MANNOSIDASE; DOLICHOL; GALACTOSE; LIPID; MANNOSE; MEVALONIC ACID; N ACETYLGALACTOSAMINE; OLIGOSACCHARIDE; SYNTHETASE; TRITIUM;

ANIMAL CELL; ARTICLE; CARBOHYDRATE ANALYSIS; CELL MUTANT; CHO CELL; ENZYME ASSAY; ENZYME PHOSPHORYLATION; FUNGAL GENETICS; GENETIC TRANSFECTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOTOPE LABELING; NONHUMAN; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE;

ANIMALS; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; GENE EXPRESSION; GLUCOSYLTRANSFERASES; GLYCOSYLATION; KINETICS; MANNOSE; MUTATION; OLIGOSACCHARIDES; POLYISOPRENYL PHOSPHATE SUGARS; PROTEINS; SACCHAROMYCES CEREVISIAE; TRANSFECTION;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; SACCHAROMYCES CEREVISIAE; TRITIUM;

EID: 0345040245     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/9.1.65     Document Type: Article

References (38)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.E., Seigman, J.G., Smith, J.A. and Struhl, K. (1987-1997) Current Protocols in Molecular Biology. Volume 1. John Wiley and Sons, New York.
2. Breuer, W. and Bause, E. (1995) Oligosaccharyl transferase is a constitutive component of an oligomeric protein complex from pig liver endoplasmic reticulum. Eur. J. Biochem., 228, 689-696.
3. Cacan, R., Villers, C., Bélard, M., Kaiden, A., Krag, S.S. and Verbert, A. (1992) Different fates of the oligosaccharide moieties of lipid intermediates. Glycobiology, 2, 127-136.
4. Cummings, R.D. (1992) Synthesis of asparagine-linked oligosaccharides: pathways, genetics, and metabolic regulation. In Allen, H.J. and Kisailus, E.C. (eds.), Glycoconjugates. Composition, Structure, and Function. Marcel Dekker, New York, pp. 333-360.
5. 2pyrophosphoryldolichol. J. Biol. Chem., 268, 4720-4727.
6. D'Souza-Schorey, C, McLachlan, K.R., Krag, S.S. and Elbein, A.D. (1994) Mammalian glycosyltransferases prefer glycosylphosphoryldolichols rather than glycosylphosphorylpolyprenols as substrates for oligosaccharyl synthesis. Arch. Biochem. Biophys., 308, 497-503.
7. Fernandez, F.S., Trombetta, S.E., Hellman, U. and Parodi, A.J. (1994) Purification to homogeneity of UDP-glucose:glycoprotein glucosyltransferase from Schizosaccharomyces pombe and apparent absence of the enzyme from Saccharomyces cerevisiae. J. Biol. Chem., 269, 30701-30706.
8. Folch, J., Lees, M. Slone-Stanley, G.H. (1957) A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem., 226, 497-509
9. Hammond, C. and Helenius, A. (1995) Quality control in the secretory pathway. Curr. Opinion Cell Biol., 7, 523-529.
10. Hubbard, S.C. and Robbins, P.W. (1979) Synthesis and processing of protein-linked oligosaccharides in vivo. J. Biol. Chem., 254, 4568-4576.
11. Jakob, C.A., Burda, P., te Heesen, S., Aebi, M. and Roth, J. (1998) Genetic tailoring of N-linked oligosaccharides: the role of glucose residues in glycoprotein processing of Saccharomyces cerevisiae in vivo. Glycobiology, 8, 155-164.
12. Kaiden, A. and Krag, S.S. (1991) Regulation of glycosylation of asparagine-linked glycoproteins. Trends Glycosci. Glycotechnol., 3, 275-286.
13. Kaiden, A. and Krag, S.S. (1992) Dolichol metabolism in Chinese hamster ovary cells. Biochem. Cell Biol., 70, 385-389.
14. Kornfeld, S., Gregory, W. and Chapman, A. (1979) Class E Thy-1 negative mouse lymphoma cells utilize an alternate pathway of oligosaccharide processing to synthesize complex-type oligosaccharides. J. Biol. Chem., 254, 11649-11654.
15. 3H]glucosyl oligosaccharide-lipid. J. Biol. Chem., 254, 9167-9177.
16. 14C]mannose with preparations of infected chicken embryo fibroblasts., J. Biol. Chem., 252, 2621-2629.
17. McLachlan, K.R. and Krag, S.S. (1994) Three enzymes involved in oligosaccharide-lipid biosynthesis assembly in Chinese hamster ovary cells differ in lipid substrate preferences. J. Lipid Res., 35, 1861-1868.
18. 3 influences both glycosylation efficiency and selection of N-linked sites. Biochim. Biophys. Acta, 1201, 361-366.
19. Murphy, L.A. and Spiro, R.G. (1981) Transfer of glucose to oligosaccharide-lipid intermediates by thyroid microsomal enzymes and its relationship to the N-glycosylation of proteins. J. Biol. Chem.. 256, 7487-7494.
20. Parodi, A.J. (1993) N-Glycosylation in trypanosomatid protozoa. Glycobiology, 3, 193-199.
21. Rassoulzadegan, M., Cowie. A., Carr, A., Glaichenhaus, N., Kames, R. and Cuzin, F. (1982) The roles of individual polyoma virus early proteins in oncogenic transformation. Nature, 300, 713-718.
22. Reiss, G., te Hessen, S., Zimmerman, J., Robbins, P.W. and Aebi, M. (1996) Isolation of the ALG6 locus of Saccharomyces cerevisiae required for glucosylation in the N-linked glycosylation pathway. Glycobiology, 6,493-498.
23. Rosenwald, A.G., Stanley, P., McLachlan, K.R. and Krag, S.S. (1993) Mutants in dolichol synthesis: conversion of polyprenol to dolichol appears to be a rate-limiting step in dolichol synthesis. Glycobiology, 3, 481-488.
24. Runge, K.W., Huffaker, T.C. and Robbins, P.W. (1984) Two yeast mutations in glucosylation steps of the asparagine glycosylation pathway. J. Biol. Chem., 259, 412-417.
25. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Second edition. Cold Spring Harbor Laboratory Press, Plainview, NY.
26. Schachter, H. (1995) Glycosyltransferases involved in the synthesis of N-glycan antennae. In Montreuil, J., Schachter, H. and Vliegenthart. J.F.G. (eds.), Glycoproteins. New Comprehensive Biochemistry, Vol. 29a. Elsevier Science, Amsterdam, pp. 153-199.
27. Sharma, C.B., Lehle, L. and Tanner, W. (1981) N-Glycosylation of yeast proteins, characterization of the solubilized oligosaccharyl transferase. Eur. J. Biochem., 116, 101-108.
28. Silberstein, S. and Gilmore, R. (1996) Biochemistry, molecular biology, and genetics of the oligosaccharyltransferase. FASEB J., 10, 849-858.
29. Sousa, M.C., Ferrero-Garcia, M.A., and Parodi, A.J. (1992) Recognition of the oligosaccharide and protein moieties of glycoproteins by the UDP-Glc:glycoprotein glucosyltransferase. Biochemistry, 31, 97-105.
30. Staneloni, R.J., Ugalde, R.A. and Leloir, L.F. (1980) Addition of glucose to dolichol diphosphate oligosaccharide and transfer to protein. Eur. J. Biochem., 105, 275-278.
31. Stoll, J. and Krag, S.S. (1988) A mutant of Chinese hamster ovary cells with a reduction in levels of dolichyl phosphate available for glycosylation. J. Biol. Chem., 263, 10766-10773.
32. Stoll, J., Cacan, R., Verbert, A. and Krag, S.S. (1992) Lec15 cells transfer glucosylated oligosaccharides to protein. Arch. Biochem. Biophys., 299, 225-231.
33. Thompson, L.H. and Baker, R.M. (1973) Isolation of mutants of cultured mammalian cells. Methods Cell Biol., 6, 209-281.
34. Treisman, R., Cowie, A., Favaloro, J., Jat, P. and Kamen, R. (1981) The structures of the spliced mRNAs encoding polyoma virus early region promoters. J. Mol. Appl. Gen., 1, 83-92.
35. Trimble, R.B., Byrd, J.C. and Maley, F. (1980) Effect of glucosylation of lipid intermediates on oligosaccharide transfer in solubilized microsomes from Saccharomyces cerevisiae. J. Biol. Chem., 255, 11892-11895.
36. Trombetta, S.E., Bosch, M. and Parodi, A.J. (1989) Glucosylation of glycoproteins by mammalian, plant, fungal, and trypanosomatid protozoa microsomal proteins. Biochemistry, 28, 8108-8116.
37. Turco, S.J., Stetson, B. and Robbins, P.W. (1977) Comparative rates of transfer of lipid-linked oligosaccharides to endogenous glycoprotein acceptors in vitro. Proc. Natl. Acad. Sci. USA. 74, 4411-4414.
38. Zhu, Z.Y. Veldman, G.M., Cowie, A., Carr, A., Schaffhausen, B. and Kamen, R. (1984) Construction and functional characterization of polyomavirus genomes that separately encode the three early proteins. J. Virol., 51, 170-180.