Transport activity of the multidrug resistance protein is accompanied by amiloride-sensitive intracellular pH changes in rat hepatoma cells

Hepatology Research

Volumn 10, Issue 1, 1998, Pages 27-40

  García Cañero, Rafael ^a   Pérez De Diego, Javier ^b   Trilla, Carolina ^a   M Cobo, José ^c   J Díaz Gil, Juan ^a  

^a Dept Exp Biochem Clin Puerta H   (Spain)

^b UNIVERSIDAD ALFONSO X EL SABIO   (Spain)

^c UNIVERSITY OF ALCALÁ   (Spain)

Author keywords

Amiloride; Multidrug resistance; Rat hepatoma cells; Rhodamine 123

Indexed keywords

AMILORIDE; COLCHICINE; DOXORUBICIN; GLYCOPROTEIN; MULTIDRUG RESISTANCE PROTEIN; PROTEIN KINASE C; RHODAMINE 123; STAUROSPORINE; VERAPAMIL;

ACIDIFICATION; ANIMAL CELL; ARTICLE; CELL MEMBRANE; CELL PH; CULTURE MEDIUM; HEPATOMA CELL; HOMEOSTASIS; IMMUNOBLOTTING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTON SODIUM EXCHANGE; RAT;

EID: 0344878864     PISSN: 09284346     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Gottesman M.M., Pastan I. Biochemistry of multidrug resistance mediated by the multidrug transporter. Ann Rev Biochem. 62:1990;385-427.
2. Arias IM. Multidrug resistance genes, P-glycoprotein and the liver. Hepatology. 12:1990;159-165.
3. Vollrath V., Wielandt A.M., Acuña C. et al. Effect of colchicine and heat shock on multidrug resistance gene and P-glycoprotein expression in rat liver. J Hepatol. 21:1994;754-763.
4. Kamimoto Y., Gadmaitan Z., Hsu J., Arias I.M. The function of Gp170, the multidrug resistance gene product, in rat liver canalicular membrane vesicles. J Biol Chem. 264:1989;11693-11698.
5. Higgins C.F. ABC transporters: from microorganisms to man. Ann Rev Biochem. 65:1993;67-113.
6. Higgins C.F., Gottesman M.M. Is the multidrug transporter a flippase? Trends Biochem Sci. 17:1992;18-21.
7. Thiebaut F., Currier S.J., Whitaker J., Haugland P., Gottesman M.M., Pastan I. Activity of multidrug transporter results in alkalinization of the cytosol: measurement of cytosolic pH by microinjection of a pH-sensitive dye. J Histochem Cytochem. 38:1990;685-690.
8. Hamilton G., Cosenteni E.P., Teleky B. et al. The multidrug-resistance modifiers verapamil, cyclosporine A and tamoxifen induce an intracellular acidification in colon carcinoma cells in vitro. Anticancer Res. 13:1993;2059-2064.
9. Roepe P.D. The role of the MDR protein in altered drug translocation across tumor cell membranes. Biochim. Biophys Acta. 1241:1995;385-406.
10. Roepe P.D., Wei L.Y., Cruz J., Carlson D. Lower electrical membrane potential and altered pHi homeostasis in multidrug-resistant (MDR) cells: further characterizacion of a series of MDR cell lines expressing different levels of P-glycoprotein. Biochemistry. 32:1993;11042-11056.
11. Thorgeirsson S.S., Huber B.E., Sorrel S. Expression of the multidrug-resistant gene in hepatocarcinogenesis and regenerating rat liver. Science. 236:1987;1120-1122.
12. i: calibration in three different types of cell preparations. J Cell Physiol. 151:1992;596-603.
13. Gazzola G.G., DallAsta V., Gazzola R.F.and, White M.F. The cluster-tray method for rapid measurement of solute fluxes in adherent cultured cells. Anal Biochem. 115:1981;368-374.
14. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:1976;248-254.
15. Emaus R.K., Grunwald R., Lemasters J.J. Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. Biochim Biophys Acta. 850:1986;436-448.
16. 2+-dependent cytotoxicity in hepatocytes. Biochem J. 299:1994;539-543.
17. 2+transport by rat liver plasma membrane vesicles. J Biol Chem. 269:1984;1382-1385.
18. Hirsch-Ernst K., Ziemann C., Schmitz-Salue H., Kahl G.F. Modulation of p-glycoprotein and mdr 1b expression by growth factors in primary rat hepatocyte culture. Biochem Biophys Res Commun. 215:1995;179-185.
19. Bates S.E., Lee J.S., Dickstein B., Spolyar M., Fojo A.T. Differential modulation of P-glycoprotein transport by protein kinase inhibition. Biochemistry. 32:1993;9156-9164.
20. Kleyman T.R., Cragoe Jr E.J. Amiloride and its analogs as tools in the study of ion transport. J Membr Biol. 105:1988;1-21.
21. +-mediated unifying approach: pH-related and pH-unrelated mechanisms. Crit Rev Oncogenesis. 6:1995;1-33.
22. Tannock I.F., Rotin D. Acid pH in solid tumors and its potential for therapeutic explotation. Cancer Res. 49:1989;4373-7384.
23. Simon S., Roy D., Schindler M. Intracellular pH and the control of multidrug resistance. Proc Natl Acad Sci USA. 91:1994;1128-1132.
24. +is activated early, transiently and at a posttranscriptional level during rat liver regeneration. Hepatology. 19:1994;1290-1301.
25. Horio M., Gottesman M.M., Pastan I. Energy dependent transport of vinblastine in vesicles from human multidrug-resistant cells. Proc Natl Acad Sci USA. 85:1988;3580-3584.
26. +exchange and growth factor-induced cytosolic pH changes. Role in cellular proliferation. Biochim Biophys Acta. 988:1989;73-97.
27. +exchange activity. Hepatology. 22:1995;588-597.
28. Gerweck L.E., Seetharaman K. Cellular pH gradient in tumor versus normal tissue.Potential exploitation for the treatment of cancer. Cancer Res. 56:1996;1194-1198.