Fluorescence-lifetime-based determination of anions using a site-directed mutant enzyme transducer

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 2980, Issue , 1997, Pages 247-257

  Thompson, Richard B ^a   Lin, Hai Jui ^a   Ge, Zhengfang ^a   Johnson, Kelly ^b   Fierke, Carol A ^b  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^b DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Biosensor; Carbonic anhydrase; Cyanate; Cyanide; Fluorescence lifetime; Fluorescence sensor

Indexed keywords

BIOSENSORS; CADMIUM; CADMIUM COMPOUNDS; CASE HARDENING; CHEMICAL BONDS; COBALT; COPPER; CYANIDES; ENERGY TRANSFER; ENZYMES; IONS; MERCURY (METAL); METAL IONS; METALS; MOLECULAR ORBITALS; NEGATIVE IONS; QUANTUM CHEMISTRY; SENSORS; SIGNAL TRANSDUCTION; SOLUTIONS; TRACE ANALYSIS; TRANSITION METALS; WAVE FUNCTIONS; ZINC;

ABSORBANCE; ABSORBANCE BANDS; ACTIVE SITES; ANALYTES; AQUEOUS SOLUTIONS; CARBONIC ANHYDRASE; CYANATE; DEGREE OF ENERGIES; ENVIRONMENTAL POLLUTANTS; FIBER OPTIC BIOSENSORS; FLUORESCENCE ENERGY TRANSFERS; FLUORESCENCE LIFETIME; FLUORESCENCE SENSOR; FLUORESCENT LABELS; HUMAN CARBONIC ANHYDRASE; INDUSTRIAL PROCESSES; LIFE-TIMES; METALLOENZYME; METHODS FOR ANALYSES; MUTANT ENZYMES; OVERLAP INTEGRALS; PAPER MANUFACTURES;

FLUORESCENCE;

EID: 0344856416     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.273524     Document Type: Conference Paper

References (29)

1. D. Ammann, Ion-Selective Microelectrodes Springer-Verlag, Berlin, 1986.
2. L. M. Nair and R. Saari-Nordhaus, "Use of a conventional HPLC system for performing U.S. EPA Method 300.0, 'Determination of Inorganic Anions by Ion Chromatography,'" Amer. Lab. 29(4), 33N (1997).
3. A. Fernandez-Gutierrez and A.M. de la Pena, "Determination of inorganic substances by luminescence methods," in Molecular Luminescence Spectroscopy, vol. 1: Methods and Applications (S.G. Schulman, Ed.), Wiley-Interscience, New York, pp. 371-546 (1986).
4. M.E. Huston, E.U. Akkaya, and A.W. Czarnik, J. Am. Chem. Soc. 111, 8735 (1989).
5. Z. Zhujun and W.R. Seitz, "A carbon dioxide sensor based on fluorescence," Anal. Chim. Acta 160, 501 (1984).
6. R.B. Thompson and D.R. Walt, "Emerging Strategies for Molecular Biosensors," ONR Research Reviews 46 (3), 19-29 (1994).
7. R.B. Thompson and E.R. Jones, "Enzyme-Based Fiber Optic Zinc Biosensor," Analytical Chemistry 65, 730-4 (1993).
8. R.B. Thompson and M.W. Patchan, "Lifetime-Based Fluorescence Energy Transfer Biosensing of Zinc," Anal. Biochem. 227, 123-128 (1995).
9. R.B. Thompson, Z. Ge, M.W. Patchan, and C.A. Fierke, "Performance enhancement of fluorescence energy transfer-based biosensors by site-directed mutagenesis of the transducer," J. Biomed. Optics 1(1), 131-137 (1996).
10. R.B. Thompson, "Fluorescence-Based Fiber Optic Sensors," in Topics in Fluorescence Spectroscopy. Vol. 2: Principles (J.R. Lakowicz, Ed.) New York: Plenum Press; pp. 345-365 (1991).
11. R.B. Thompson, Z. Ge, M.W. Patchan, C.-C. Huang, and C.A. Fierke, "Fiber optic biosensor for Co(II) and Cu(II) based on fluorescence energy transfer with an enzyme transducer," Biosensors and Bioelectronics 11 (6/7), 557-564 (1996).
12. D. Elbaum, S.K. Nair, M.W. Patchan, R.B. Thompson, and D.W. Christianson, "Structure-based design of a sulfonamide probe for fluorescence anisotropy detection of zinc with a carbonic anhydrase-based biosensor," J. Am. Chem. Soc. 118, 8381-7 (1996).
13. S. Lindskog, L.E. Henderson, K.K. Kannan, A. Liljas, P.O. Nyman, and B. Strandberg, "Carbonic anhydrase" in The Enzymes. Fifth Edition (P.D. Boyer, Ed.) New York, Academic Press, pp. 587-665 (1971).
14. Lindskog, S., and Nyman, P.O. (1964) "Metal binding properties of human erythrocyte carbonic anhydrase," Biochim. Biophys. Acta 85, 462-474.
15. J. Coleman, "Human carbonic anhydrase. Protein conformation and metal ion binding," Biochemistry 4, 2644-2655 (1965).
16. S. Lindskog, "Effects of pH and inhibitors on some properties related to metal binding in bovine carbonic anhydrase," J. Biol. Chem. 238, 945-951 (1963).
17. A. Thorslund and S. Lindskog, "Studies of the esterase activity and the anion inhibition of bovine zinc and cobalt carbonic anhydrases," Europ. J. Biochem. 3, 117-123 (1967).
18. R. Einarsson and M. Zeppezauer, "Visible absorption and circular dichroism spectra of 5-(4′-sulfamylphenylazo)-8-hydroxyquinoline bound to carbonic anhydrase and alcohol dehydrogenase," Acta Chem. Scand. 24, 1098-1101 (1970).
19. Th. Forster, "Intermolecular energy migration and fluorescence," Ann. Physik 2, 55-75 (1946).
20. J.F. Krebs, F. Rana, R.A. Dluhy, and C.A. Fierke, "Kinetic and spectroscopic studies of hydrophilic amino acid substitutions in the hydrophobic pocket of human carbonic anhydrase II, Biochemistry 32, 9896-9900 (1993).
21. J.B. Hunt, M.-J. Rhee, and C.B. Storm, "A rapid and convenient preparation of apocarbonic anhydrase," Anal. Biochem. 79, 614-7 (1977).
22. R.S. Alexander, L.L. Kiefer, C.A. Fierke, and D.W. Christianson, "Engineering the zinc binding site of human carbonic anhydrase II: Structure of the His-94-Cys apoenzyme in a new crystalline form," Biochemistiy 32, 1510-1518 (1993).
23. R.B. Thompson and E. Gratton, "Phase Fluorimetric Method for Determination of Standard Lifetimes," Anal. Chem. 60, 670-674 (1988).
24. Y. Kidani and J. Hirose, Chem. Lett. (Tokyo), 475 (1977).
25. H. Szmacinski and J.R. Lakowicz, "Optical measurements of pH using fluorescence lifetimes and phase-modulation fluorometry," Anal. Chem. 65, 1668-1674 (1993).
26. R.B. Thompson and M.W. Patchan, "Fluorescence Lifetime-Based Biosensing of Zinc: Origin of the Broad Dynamic Range," J. Fluoresc. 5, 123-130 (1995).
27. C.A. Fierke, T. Calderone, J.F. Krebs, "Functional consequences of engineering the hydrophobic pocket of carbonic anhydrase II," Biochemistry 31, 11054-11063 (1991).
28. J.F. Krebs, J.A. Ippolito, D.W Christianson, and C.A. Fierke, "Structural and functional importance of a conserved hydrogen bond network in human carbonic anhydrase II," J. Biol. Chem. 268 27458-27466 (1993).
29. S.K. Bhatia, L.C. Shriver-Lake, K.J. Prior, J.H. Georger, J.M. Calvert, R. Bredehorst, and F.S. Ligler, "Use of thiol-terminal silanes and heterobifunctional cross-linkers for immobilization of antibodies on silica surfaces," Anal. Biochem. 178, 408-413 (1989).