Performance enhancement of fluorescence energy transfer-based biosensors by site-directed mutagenesis of the transducer

Journal of Biomedical Optics

Volumn 1, Issue 1, 1996, Pages 131-137

  Thompson, Richard B ^a   Ge, Zhengfang ^a   Patchan, Marcia W ^a   Fierke, Carol A ^b  

^a UNIVERSITY OF MARYLAND   (United States)

^b DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Biosensors; Fluorescence energy transfer; Fluorometry; Mutagenesis; Transducers

Indexed keywords

EID: 0000057588     PISSN: 10833668     EISSN: None     Source Type: Journal    
DOI: 10.1117/12.227108     Document Type: Article

References (34)

1. R.B. Thompson and D.R. Walt, "Emerging strategies for molecular biosensors," ONR Research Rev. 46 (3), 19-29 (1994).
2. O.S. Wolfbeis, Ed., Fiber Optic Chemical Sensors and Biosensors, CRC Press, Boca Raton, FL (1991).
3. D.L. Wise and L. Wingard, Eds. Biosensors with Fiber Optics, Humana Press, Clifton, NJ (1991).
4. J.R. Lakowicz and R.B. Thompson, Eds. Advances in Fluorescence Sensing Technology, Proc. SPIE 1885 (1993).
5. J.R. Lakowicz, Ed. Advances in Fluorescence Sensing Technology II Proc. SPIE 2388 (1995).
6. R.B. Thompson and E.R. Jones, "Enzyme-based fiber optic zinc biosensor," Anal. Chem. 65, 730-734 (1993).
7. L.C. Shriver-Lake, K.A. Breslin, P.T. Charles, D.W. Conrad, J.P. Golden, and F.S. Ligler, "Detection of TNT in water using an evanescent wave biosensor," Anal. Chem. 67, 2431-2435 (1995).
8. R.A. Ogert, J.E. Brown, B.R. Singh, L.C. Shriver-Lake, and F.S. Ligler, "Detection of Clostridium botulinum toxin A using a fiber optic-based biosensor," Anal. Biochem. 205, 306-312 (1992).
9. B.J. Tromberg, M.J. Sepaniak, T. Vo-Dinh, and G.D. Griffin, "Fiber optic chemical sensors for competitive-binding fluoroimmunoassay," Anal. Chem. 59, 1226-1230 (1987).
10. L.A. Saari and W.R. Seitz, "Immobilized morin as a fluorescence sensor for Al(III)," Anal. Chem. 55, 667-670 (1983).
11. P. Yager, R.B. Thompson, and S. Merlo, "Fluorescence-based optical sensor and method for detection of lipid-soluble analytes," U.S. Patent No. 5,094,819 (1992).
12. M.E. Lippitsch, J. Pusterhofer, M.J.P. Leiner, and O.S. Wolfbeis, "Fibre-optic oxygen sensor with the fluorescence decay time as the information carrier," Anal. Chim. Acta 207, 1-6 (1988).
13. J.R. Lakowicz, "Fluorescence lifetime-based sensing," Laser Focus World 28 (5), 127 (1992).
14. H. Szmacinski and J.R. Lakowicz, "Lifetime-based sensing," in Topics in Fluorescence Spectroscopy, Vol. 4: Probe Design and Chemical Sensing, J.R. Lakowicz, Ed., pp. 295-334, Plenum Press, New York (1994).
15. H. Szmacinski and J.R. Lakowicz, "Optical measurements of pH using fluorescence lifetimes and phase-modulation fluorometry," Anal. Chem. 65, 1668-1674 (1993).
16. R.B. Thompson and M.W. Patchan, "Fluorescence lifetime-based biosensing of zinc: Origin of the broad dynamic range," J. Fluoresc. 5, 123-130 (1995).
17. R.B. Thompson, "Fluorescence-based fiber optic sensors," in Topics in Fluorescence Spectroscopy, Vol. 2: Principles, J.R. Lakowicz, Ed., pp. 345-365, Plenum Press, New York (1991).
18. J.R. Lakowicz, H. Szmacinski, and R.B. Thompson, "Fluorescence lifetime-based sensing: Applications to clinical chemistry and cellular imaging," in Ultrasensitive Laboratory Diagnostics, G.E. Cohn, Ed., Proc. SPIE 1895, 2-17 (1993).
19. R.B. Thompson and M.W. Patchan, "Lifetime-based fluorescence energy transfer biosensing of zinc," Anal. Biochem. 227, 123-128 (1995).
20. R.B. Thompson, Z. Ge, M.W. Patchan, and C.A. Fierke, "Energy transfer-based fiber optic metal ion biosensor," Advances in Fluorescence Sensing Technology, J.R. Lakowicz, Ed., Proc. SPIE 2388, 138-147 (1995).
21. 2 gas sensor with blue or red excitation and Stokes or anti-Stokes detection," Anal. Chem. 227, 309-318 (1995).
22. J.R. Lakowicz, B.P. Maliwal, A. Ozinskas, and R.B. Thompson, "Fluorescence lifetime energy-transfer immunoassay quantified by phase-modulation fluorometry," Sensors and Actuators B 12, 65-70 (1993).
23. C.E. White and R.J. Argauer, Fluorescence Analysis: A Practical Approach, Marcel Dekker, New York (1970).
24. R.D. Spencer and G. Weber, "Cross-correlation phase fluorometer," Ann. N.Y. Acad. Sci. 158, 361-376 (1969).
25. J.R. Lakowicz and I. Gryczynski, "Frequency-domain fluorescence spectroscopy," in Topics in Fluorescence Spectroscopy Vol. 1: Techniques, J.R. Lakowicz, Ed., Chapter 5, pp. 293-335, Plenum Press, New York (1991).
26. J.E. Coleman, "Carbonic anhydrase-azosulfonamide complexes. Optical rotatory dispersion and circular dichroism," J. Am. Chem. Soc. 89, 6757-6759 (1967).
27. J.R. Lakowicz, I. Gryczynski, W. Wiczk, J. Kusba, and M.L. Johnson, "Correction for incomplete labeling in the measurement of distance distributions by frequency-domain fluorometry," Anal. Biochem. 195, 243-254 (1991).
28. J.F. Krebs, F. Rana, R.A. Dluhy, and C.A. Fierke, "Kinetic and spectroscopic studies of hydrophilic amino acid substitutions in the hydrophobic pocket of human carbonic anhydrase II, Biochemistry 32, 9896-9900 (1993).
29. J.B. Hunt, M.-J. Rhee, and C.B. Storm, "A rapid and convenient preparation of apocarbonic anhydrase," Anal. Biochem. 79, 614-617 (1977).
30. R.B. Thompson and E. Gratton, "Phase fluorimetric method for determination of standard lifetimes," Anal. Chem. 60, 670-674 (1988).
31. D. Davenport, "Use of fluorescence in binding studies," in Fluorescence Spectroscopy: An Introduction for Biology and Medicine, A.J. Pesce, C.-G. Rosen, and T.L. Pasby, Eds., pp. 203-240, Marcel Dekker, New York (1971).
32. H.A. Krebs, Biophys. J. 43, 525-528 (1948).
33. R.S. Alexander, L.L. Kiefer, C.A. Fierke, and D.W. Christianson, "Engineering the zinc binding site of human carbonic anhydrase II: Structure of the his-94 to cys apoenzyme in a new crystalline form," Biochemistry 32, 1510-1518 (1993).
34. E.F. Ullman and M. Schwarzberg, "Fluorescence quenching with immunological pairs in immunoassays," U.S. Patent No. 4,261,968 (1981).