Quantification of sphingosine and sphinganine from crude lipid extracts by HPLC electrospray ionization tandem mass spectrometry

Journal of Lipid Research

Volumn 44, Issue 11, 2003, Pages 2209-2216

  Lieser, Bernd ^a   Liebisch, Gerhard ^a   Drobnik, Wolfgang ^a   Schmitz, Gerd ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

High throughput; High performance liquid chromatography; Metabolism; Sphingolipids

Indexed keywords

LIPID; SPHINGANINE; SPHINGOID; SPHINGOLIPID; SPHINGOSINE; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; AUTOMATION; CALIBRATION; CELL DIFFERENTIATION; CELL GROWTH; CONTROLLED STUDY; DATA ANALYSIS; DIAGNOSTIC ACCURACY; ELECTROSPRAY IONIZATION TANDEM MASS SPECTROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY; HUMAN; HUMAN CELL; LIPID METABOLISM; METHODOLOGY; PRIORITY JOURNAL; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; SECOND MESSENGER; SENSITIVITY AND SPECIFICITY; SIGNAL TRANSDUCTION; STANDARD; TANDEM MASS SPECTROMETRY;

ANIMALS; CELL EXTRACTS; CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FIBROBLASTS; FORMIC ACIDS; HUMANS; LIPIDS; MASS SPECTROMETRY; MICE; SENSITIVITY AND SPECIFICITY; SPHINGOSINE;

EID: 0344667483     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: 10.1194/jlr.D300025-JLR200     Document Type: Article

References (21)

1. Gomez-Munoz, A. 1998. Modulation of cell signalling by ceramides. Biochim. Biophys. Acta. 1391: 92-109.
2. Hannun, Y. A., C. Luberto, and K. M. Argraves. 2001. Enzymes of sphingolipid metabolism: from modular to integrative signaling. Biochemistry. 40: 4893-4903.
3. Huwiler, A., T. Kolter, J. Pfeilschifter, and K. Sandhoff. 2000. Physiology and pathophysiology of sphingolipid metabolism and signaling. Biochim. Biophys. Acta. 1485: 63-99.
4. Maceyka, M., S. G. Payne, S. Milstien, and S. Spiegel. 2002. Sphingosine kinase, sphingosine-1-phosphate, and apoptosis. Biochim. Biophys. Acta. 1585: 193-201.
5. Merrill, A. H., Jr., E. M. Schmelz, D. L. Dillehay, S. Spiegel, J. A. Shayman, J. J. Schroeder, R. T. Riley, K. A. Voss, and E. Wang. 1997. Sphingolipids - the enigmatic lipid class: biochemistry, physiology, and pathophysiology. Toxicol. Appl. Pharmacol. 142: 208-225.
6. Merrill, A. H., Jr., E. Wang, R. E. Mullins, W. C. Jamison, S. Nimkar, and D. C. Liotta. 1988. Quantitation of free sphingosine in liver by high-performance liquid chromatography. Anal. Biochem. 171: 373-381.
7. Min, J. K., H. S. Yoo, E. Y. Lee, W. J. Lee, and Y. M. Lee. 2002. Simultaneous quantitative analysis of sphingoid base 1-phosphates in biological samples by o-phthalaldehyde precolumn derivatization after dephosphorylation with alkaline phosphatase. Anal. Biochem. 303: 167-175.
8. Ohta, H., F. Ruan, S. Hakomori, and Y. Igarashi. 1994. Quantification of free sphingosine in cultured cells by acylation with radioactive acetic anhydride. Anal. Biochem. 222: 489-494.
9. Edsall, L. C., and S. Spiegel. 1999. Enzymatic measurement of sphingosine 1-phosphate. Anal. Biochem. 272: 80-86.
10. Olivera, A., J. Rosenthal, and S. Spiegel. 1994. Sphingosine kinase from Swiss 3T3 fibroblasts: a convenient assay for the measurement of intracellular levels of free sphingoid bases. Anal. Biochem. 223: 306-312.
11. Van Veldhoven, P. P., W. R. Bishop, and R. M. Bell. 1989. Enzymatic quantification of sphingosine in the picomole range in cultured cells. Anal. Biochem. 183: 177-189.
12. Mano, N., Y. Oda, K. Yamada, N. Asakawa, and K. Katayama. 1997. Simultaneous quantitative determination method for sphingolipid metabolites by liquid chromatography/ionspray ionization tandem mass spectrometry. Anal. Biochem. 244: 291-300.
13. Sullards, M. C. 2000. Analysis of sphingomyelin, glucosylceramide, ceramide, sphingosine, and sphingosine 1-phosphate by tandem mass spectrometry. Methods Enzymol. 312: 32-45.
14. Seefelder, W., G. Schwerdt, R. Freudinger, M. Gekle, and H. U. Humpf. 2002. Liquid chromatography/electrospray ionisation-mass spectrometry method for the quantification of sphingosine and sphinganine in cell cultures exposed to fumonisins. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 780: 137-144.
15. Bligh, E. G., and W. J. Dyer. 1959. A rapid method of total lipid extraction and purification. Can. J. Med Sci. 37: 911-917.
16. Smith, P. K., R. I. Krohn, G. T. Hermanson, A. K. Mallia, F. H. Gartner, M. D. Provenzano, E. K. Fujimoto, N. M. Goeke, B. J. Olson, and D. C. Klenk. 1985. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150: 76-85. [Erratum. 1987. Anal. Biochem. 163: 279.]
17. Smith, P. K., R. I. Krohn, G. T. Hermanson, A. K. Mallia, F. H. Gartner, M. D. Provenzano, E. K. Fujimoto, N. M. Goeke, B. J. Olson, and D. C. Klenk. 1985. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150: 76-85. [Erratum. 1987. Anal. Biochem. 163: 279.]
18. Desai, K., M. C. Sullards, J. Allegood, E. Wang, E. M. Schmelz, M. Hartl, H. U. Humpf, D. C. Liotta, Q. Peng, and A. H. Merrill, Jr. 2002. Fumonisins and fumonisin analogs as inhibitors of ceramide synthase and inducers of apoptosis. Biochim. Biophys. Acta. 1585: 188-192.
19. Sonnino, S., and V. Chigorno. 2000. Ganglioside molecular species containing C18- and C20-sphingosine in mammalian nervous tissues and neuronal cell cultures. Biochim. Biophys. Acta. 1469: 63-77.
20. Lavie, Y., J. K. Blusztajn, and M. Liscovitch. 1994. Formation of endogenous free sphingoid bases in cells induced by changing medium conditions. Biochim. Biophys. Acta. 1220: 323-328.
21. Smith, E. R., and A. H. Merrill, Jr. 1995. Differential roles of de novo sphingolipid biosynthesis and turnover in the "burst" of free sphingosine and sphinganine, and their 1-phosphates and N-acylderivatives, that occurs upon changing the medium of cells in culture. J. Biol. Chem. 270: 18749-18758.