Characterisation of the lpdA gene from Neisseria meningitidis by polymerase chain reaction, restriction fragment length polymorphism and sequencing

FEMS Microbiology Letters

Volumn 174, Issue 1, 1999, Pages 191-199

  Silva, Ricardo ^a   Menéndez, Tamara ^a   Alonso, Luis M ^a   Iglesias, Enrique ^a   Musacchio, Alexis ^a   Leal, María De Jesús ^a   Alvarez, Anabel ^a   Coizeau, Edelgis ^a   Martín, Alejandro ^a   Herrera, Luis ^a   Guillén, Gerardo ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

lpdA gene; Neisseria meningitidis; Polymerase chain reaction; Restriction fragment length polymorphism

Indexed keywords

BACTERIAL PROTEIN; MENINGOCOCCUS VACCINE; RESTRICTION ENDONUCLEASE; UNCLASSIFIED DRUG; VA MENGOC BC;

ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATE; CONTROLLED STUDY; CONVALESCENCE; GENE AMPLIFICATION; GENE SEQUENCE; GEOGRAPHIC DISTRIBUTION; MENINGOCOCCOSIS; NEISSERIA MENINGITIDIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SEROTYPE;

AMINO ACID SEQUENCE; ANTIGENS, BACTERIAL; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL VACCINES; CONSERVED SEQUENCE; GENES, BACTERIAL; MENINGOCOCCAL VACCINES; MOLECULAR SEQUENCE DATA; NEISSERIA MENINGITIDIS; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SPECIES SPECIFICITY;

EID: 0344609700     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(99)00140-8     Document Type: Article

References (39)

1. Frasch, C.E. (1995) Meningococcal vaccines: past, present and future. In: Meningococcal Disease (Cartwright K., Ed.), pp. 245-283. John Wiley, Chichester.
2. Poolman, J.T. (1995) Development of a meningococcal vaccine. Infect Agents Dis. 4, 13-28.
3. Ala'Aldeen, D.A.A. (1996) Vaccines against Neisseria meningitidis: past, present and future. Biotecnol. Apl. 13, 1-7.
4. Peltola, H., Sarkary, A., Kayhty, H., Karanko, V. and Andre F.E. (1985) Evaluation of 2 tetravalent (ACYW135) meningococcal vaccines in infant and small children, a clinical study comparing immunogenicity of O-acetyl-negative and O-acetyl-positive group C polysaccharides. Pediatrics 76, 91-96.
5. Reingold, A.L., Broome, C.V., Hightower, A.W., Ajello, G.W., Bolan, G.A., Adamsbaum, C., Jones, E.E., Phillips, C., Tiendrebeogo, H. and Yada, A. (1985) Age-specific differences in duration of clinical protection after vaccination with polysaccharide A vaccine. Lancet ii, 114-118.
6. Sierra, G.V.C., Campa, H.C., Varcacel, N.M., Garcia, I., Izquierdo, P.L., Sotolongo, P.F., Casanueva, G.V., Rico, C.O., Rodriguez, C.R. and Terry, M.H. (1991) Vaccine against group B Neisseria meningitidis: protection trial and mass vaccination result in Cuba. NIPH Ann. 14, 195-207.
7. Rosenqvist, E., Høiby, E.A., Bjune, G., Brynk, K., Closs, O., Feiring, B., Klemm, A., Nøkleby, H. and Frøholm, L.O. (1991) Human antibody responses after vaccination with the norwegian group B meningococcal outer membrane vesicle vaccine: results from ELISA studies. NIPH Ann. 14, 169-179.
8. Zollinger, W.D., Boslego, J., Moran, E., García, J., Cruz, C., Ruiz, S., Brandt, B., Martinez, M., Arthur, J., Underwood, P., Hankins, W., Mays, J., Gilly, G. and the Chilean National Committee for Meningococcal Disease (1991) Meningococcal serogroup B vaccine protection trial and follow-up studies in Chile. NIPH Ann. 14, 211-213.
9. Díaz, M.R., Rodríguez, M.F. and Baly, A. (1994) Enfermedades de declaración obligatoria seleccionadas, acumuladas hasta 15/10/94. Bol. Epidemiol. IPK 4, 443-440.
10. Tsai, C.M., Frasch, C.E. and Mocca, L.F. (1981) Five structural classes of major outer membrane proteins in Neisseria meningitidis. J. Bacteriol. 146, 67-68.
11. Mocca, L.F. and Frasch, C.E. (1982) Sodium dodecyl sulfate polyacrylamide gel electrophoresis typing system for characterisation of Neisseria meningitidis isolates. J. Clin. Microbiol. 16, 240-244.
12. Frasch, C.E., Zollinger, W.D. and Poolman, J.T. (1985) Serotype antigens of Neisseria meningitidis and a proposed scheme for designation of serotypes. Rev. Infect. Dis. 7, 504-510.
13. Abdillahi, H. and Poolman, J.T. (1988) Definition of meningococcal Class 1 OMP subtyping antigens by monoclonal antibodies. FEMS Microbiol. Immunol. 1, 139-144.
14. Ala'Aldeen, D.A.A., Westphal, A.H., de Kok, A., Weston, V., Atta, M.S., Baldwin, T.J., Bartley, J. and Borriello, S.P. (1996) Cloning, sequencing, characterisation and implications for vaccine design of the novel dihydrolipoyl acetyltransferase of Neisseria meningitidis. J. Med. Microbiol. 45, 419-432.
15. Silva, R., Selman, M., Guillén, G., Herrera, L., Fernández, J.R., Novoa, L.I., Morales, J., Morera, V., González, S., Tamargo, B., del Valle, J., Caballero, E., Alvarez, A., Coizeau, E., Cruz, S. and Musacchio, A. (1992) Nucleotide sequence coding for an outer membrane protein from Neisseria meningitidis and use of said protein in vaccine preparations. European Patent Application EP 0474313 A2.
16. Bringas, R. and Fernández, J.R. (1995) A lipoamide dehydrogenase from Neisseria meningitidis has a lipoyl domain. Protein Struct. Funct. Genet. 21, 303-306.
17. Li de la Sierra, I., Pernot, L., Prangé, T., Saludjian, P., Schiltz, M., Fourme, R. and Padrón, G. (1997) Molecular structure of the lipoamide dehydrogenase domain of a surface antigen from Neisseria meningitidis. J. Mol. Biol. 269, 129-141.
18. González, S., Nazábal, C., Viña, L. and Caballero, E. (1998) Influence of several adjuvants on the immune response against a recombinant meningococcal high molecular mass antigen. In: Modulation of the Immune Response to Vaccine Antigens (Brown, F. and Haaheim, L.R., Eds.), Vol. 92, pp. 269-276. Dev. Biol. Stand., Karger, Basel.
19. Guillén, G., Alvarez, A., Silva, R., Morera, V., González, S., Musacchio, A., Besada, V., Coizeau, E., Caballero, E., Nazábal, C., Carmenate, T., González, L.J., Estrada, R., Támbara, Y., Padrón, G. and Herrera, L. (1998) Expression in Escherichia coli of the lpdA gene, protein sequence analysis and immunological characterization of the P64k protein from Neisseria meningitidis. Biotechnol. Appl. Biochem 27, 189-196.
20. Caugant, D.A., Mocca, L.F., Frasch, C.E., Frøholm, L.O., Zollinger, W.D. and Selander, R.K. (1987). Genetic structure of Neisseria meningitidis populations in relation to serogroup, serotype, and outer membrane protein pattern. J. Bacteriol. 169, 2781-2792.
21. Wedege, E., Høiby, E.A., Rosenqvist, E. and Bjune, G. (1998) Immune response against major outer membrane antigens of Neisseria meningitidis in vaccinees and controls who contracted meningococcal disease during the Norwegian serogroup B protection trial. Infect. Immun. 66, 3232-3231.
22. Bullock, W.O., Fernández, J.M. and Stuart, J.M. (1987) XL1-Blue: a high efficiency plasmid transforming Escherichia coli strain with β-galactosidase selection. BioTechniques 5, 376-379.
23. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
24. Birnboim, H.C. and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7, 1513-1523.
25. Guillén, G., Alvarez, A., Lemos, G., Paredes, T., Silva, R. and Martin, A. (1993) Comparison of the DNA sequence of nine different genes for the class 1 outer membrane protein from Neisseria meningitidis. Biotecnol. Apl. 10, 108-113.
26. Southern, E.M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503-508.
27. Martín, A., González, S., Caballero, E., Nogueiras, E.C. (1996) Molecular characterization of the pyruvate dehydrogenase gene cluster of Neisseria meningitidis. In : Proceedings of the Tenth International Pathogenic Neisseria Conference (Zollinger, W.D., Frasch, C.E. and Deal, C.D., Eds.). Baltimore, MD.
28. Taylor, L..A. and Rose, R.E. (1988) A correction in the nucleotide sequence of the Tn903 kanamycin resistance determinant in pUC4K. Nucleic Acids Res. 16, 358-358.
29. Yanisch, P.C., Vieira, J. and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 23, 103-119.
30. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
31. Thompson, J.D., Higgins, D. G. and Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680.
32. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
33. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
34. Wedege, E. and Frøholm, L.O. (1986) Human antibody response to a group B serotype 2a meningococcal vaccine determined by immunoblotting. Infect. Immun. 51, 571-578.
35. Nazábal, C., González, S., Viña, L., Chinea, G., Caballero, E. and Mussachio, A. (1996) Analysis of the continuous epitopes recognized by antibodies against a recombinant meningococcal high molecular mass antigen. In: Proceedings of the Tenth International Pathogenic Neisseria Conference (Zollinger, W.D., Frasch, C.E. and Deal, C.D., Eds.), pp. 174-175. Baltimore, MD.
36. Kertesz, D.A., Byrne, S.K. and Chow, A.W. (1993) Characterization of Neisseria meningitidis by polymerase chain reaction and restriction endonuclease digestion of the por A gene. J. Clin. Microbiol. 31, 2594-2598.
37. Peixuan, Z., Xujing, H. and Li, X. (1995) Typing Neisseria meningitidis by analysis of restriction fragment length polymorphism in the gene encoding the Class 1 outer membrane protein: application to assessment of epidemics throughout the last four decades in China. J. Clin. Microbiol. 33, 458-462.
38. Chon, S. (1990) Differentiation of cytomegalovirus strains by restriction analysis of DNA sequences amplified from clinical specimens. J. Infect. Dis. 162, 738-742.
39. Bringas, R., Ricardo, R., Fernández de Cossío, J., Ochagavía, M., Suárez, A. and Rodríguez, R. (1992) BioSOS: A program package for the analysis of biological sequences in the microcomputer. Biotecnol. Apl. 9, 180-185.