Cloning, sequencing, characterisation and implications for vaccine design of the novel dihydrolipoyl acetyltransferase of Neisseria meningitidis

Journal of Medical Microbiology

Volumn 45, Issue 6, 1996, Pages 419-432

  Ala' Aldeen, D A A ^a   Westphal, A H ^b   De Kok, A ^b   Weston, Vivienne ^a   Atta, M S ^a   Baldwin, T J ^a   Bartley, Julia ^e   Borriello, S P ^c,d  

^a QUEEN S MEDICAL CENTRE   (United Kingdom)

^b WAGENINGEN UNIVERSITY   (Netherlands)

^c DURHAM UNIVERSITY   (United Kingdom)

^d CENTRAL PUBLIC HEALTH LABORATORY   (United Kingdom)

^e NONE

Author keywords

[No Author keywords available]

Indexed keywords

ACYLTRANSFERASE; VACCINE;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ANALYSIS; IMMUNOGENICITY; MOLECULAR CLONING; NEISSERIA MENINGITIDIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DOMAIN;

EID: 10544243793     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/00222615-45-6-419     Document Type: Article

References (39)

1. Ala'Aldeen DAA, Griffiths E. Vaccines against meningococcal diseases. In: Ala'Aldeen DAA, Hormaeche CE (eds) Molecular and clinical aspects of bacterial vaccine development. Chichester, John Wiley and Sons Ltd. 1995: 1-39.
2. Bjune G, Høiby EA, Grønnesby JK et al. Effect of outer membrane vesicle vaccine against group B meningococcal disease in Norway. Lancet 1991; 338: 1093-1096.
3. Sierra GVG, Campa HC, Varcacel NM et al. Vaccine against group B Neisseria meningitidis: protection trial and mass vaccination results in Cuba. NIPH Ann 1991; 14: 195-210.
4. De Moraes JC, Perkins BA, Camargo MCC et al. Protective efficacy of a serogroup B meningococcal vaccine in Sao Paulo, Brazil. Lancet 1992; 340: 1074-1078.
5. Ala'Aldeen DA, Wall RA, Borriello SP. Immunogenicity and cross-reactivity of the 70-kDa iron-regulated protein of Neisseria meningitidis in man and animals. J Med Microbiol 1990; 32: 275-281.
6. Ala'Aldeen DAA, Davies HA, Borriello SP. Vaccine potential of meningococcal FrpB: studies on surface exposure and functional attributes of common epitopes. Vaccine 1994; 12: 535-541.
7. Pettersson A, Kuipers B, Pelzer M et al. Monoclonal antibodies against the 70 kDa iron-regulated protein of Neisseria meningitidis are bactericidal and strain specific. Infect Immun 1990; 58: 3036-3041.
8. Pettersson AM, Maas A, van Wassenaar D, van der Ley P, Tommassen J. Molecular characterization of FrpB, the 70-kilodalton iron-regulated outer membrane protein of Neisseria meningitidis. Infect Immun 1995; 63: 4181-4184.
9. Aoun L, Cremieux AC, Casin I, Morel P, Martin PM, Serum antibody response to the 70,000-molecular-weight neisserial common antigen in humans infected by Neisseria gonorrhoeae. J Clin Microbiol 1988; 26: 1898-1900.
10. Ala'Aldeen DA, Davies HA, Wall RA, Borriello SP. The 70 kilodalton iron regulated protein of Neisseria meningitidis is not the human transferrin receptor. FEMS Microbiol Lett 1990; 69: 37-42.
11. Gibson TJ. Studies on the Epstein-Barr virus genome. PhD Thesis, University of Cambridge, 1984.
12. Palmer HM, Powell NBL, Ala'Aldeen DA, Wilton J, Borriello SP. Neisseria meningitidis transferrin-binding protein 1 expressed in Escherichia coli is surface exposed and binds human transferrin. FEMS Microbiol Lett 1993; 110: 139-146.
13. Griffiths E, Stevenson P, Ray A. Antigenic and molecular heterogeneity of the transferrin-binding protein of Neisseria meningitidis. FEMS Microbiol Lett 1990; 69: 31-36.
14. Ala'Aldeen DA, Stevenson P, Griffiths E et al. Immune responses in human and animals to meningococcal transferrin-binding proteins: implications for vaccine design. Infect Immun 1994; 62:-2984-2900.
15. Ausubel FM. Current protocols in molecular biology. New York, John Wiley and Sons, 1987.
16. Schwartz ER, Reed LJ. Alpha-keto acid dehydrogenase complexes. XII. Effects of acetylation on the activity and structure of the dihydrolipoyl transacetylase of Escherichia coli. J Biol Chem 1969; 244: 6074-6079.
17. Reed LJ, Koike M, Levitch ME.,Leach FR. Studies on the nature and reactions of protein-bound lipoic acid. J Biol Chem 1958; 232: 143-158.
18. Goa J. A micro-biuret method for protein determination of total protein in cerebrospinal fluid. Scand J Clin Lab Invest 1953; 5: 218-222.
19. Bensadoun A, Weinstein D. Assays of proteins in the presence of interfering materials. Anal Biochem 1976; 70: 241-250.
20. Stephens PE, Darlison MG, Lewis HM, Guest JR. The pyruvate dehydrogenase complex of Escherichia coli K12. Nucleotide sequence encoding the dihydrolipoamide acetyl-transferase component. Eur J Biochem 1983; 133: 481-489.
21. Hanemaaijer R, Janssen A, de Kok A, Veeger C. The dihydrolipoyltransacetylase component of the pyruvate dehydrogenase complex from Azotobacter vinelandii. Molecular cloning and sequence analysis. Eur J Biochem 1988; 174: 593-599.
22. Hein S, Steinbüchel A. Biochemical and molecular characterization of the Alcaligenes eutrophus pyruvate dehydrogenase complex and identification of a new type of dihydrolipoamide dehydrogenase. J Bacteriol 1994; 176: 4394-4408.
23. Silva R, Selman M, Guillen G et al. Nucleotide sequence coding for an outer membrane protein from Neisseria meningitidis and use of said protein in vaccine preparations. European Patent Application 1991; 0 474 313 A2.
24. Beucher M, Sparling PF. Cloning, sequencing, and characterization of the gene encoding FrpB, a major iron-regulated, outer membrane protein of Neisseria gonorrhoeae. J Bacteriol 1995; 177: 2041-2049.
25. Hanemaaijer R, Westphal AH, Berg A, van Dongen W. de Kok A, Veeger C. The gene encoding dihydrolipoyl transacetylase from Azotobacter vinelandii. Expression in Escherichia coli and activation and isolation of the protein. Eur J Biochem 1989; 181: 47-53.
26. Schulze E, Westphal AH, Veenhuis M, de Kok A. Purification and cellular localization of wild-type and mutated dihydroli-poyltransacetylases from Azotobacter vinelandii and Escherichia coli expressed in E. coli. Biochim Biophys Acta 1992; 1120: 87-96.
27. Guest JR, Angier SJ, Russell GC. Structure, expression, and protein engineering of the pyruvate dehydrogenase complex of Escherichia coli. Ann N Y Acad Sci 1989; 573: 76-99.
28. Mattevi A, Obmolova G, Schulze E et al. Atomic structure of the cubic core of the pyruvate dehydrogenase multienzyme complex. Science 1992; 255: 1544-1550.
29. Robien MA, Clore M, Omichinski JG et al. Three-dimensional solution structure of the E3-binding domain of the dihydrolipoamide succinyltransferase core from the 2-oxoglutarate dehydrogenase multienzyme complex of Escherichia coli. Biochemistry 1992; 31: 3463-3471.
30. Berg A, de Kok A, Vervoort J. Sequential 1H and 15N nuclear magnetic resonance assignments and secondary structure of the N-terminal lipoyl domain of the dihydrolipoyl transacetylase component of the pyruvate dehydrogenase complex from Azotobacter vinelandii. Eur J Biochem 1994; 221: 87-100.
31. Westfal AH, Fabisz-Koyvska A, Kester H, Obels PP, de Kok A. The interaction between lipoamide dehydrogenase and the peripheral-component-binding domain from the Azotabacter vinelandii pyruvate dehydrogenase complex. Eur J Biochem 1996; 234: 861-870.
32. de Kok A, Westphal AH. Hybrid pyruvate dehydrogenase complexes reconstituted from components of the complexes from Escherichia coli and Azotobacter vinelandii. Eur J Biochem 1985; 152: 35-41.
33. Hendle J, Mattevi A, Westphal AH et al. Crystallographic and enzymatic investigations on the role of Ser558, His610, and Asn614 in the catalytic mechanism of Azotobacter vinelandii dihydrolipoamide acetyltransferase (E2p). Biochemistry 1995; 34: 4287-4298.
34. Richarme G. Purification of a new dihydrolipoamide dehydrogenase from Escherichia coli. J Bacteriol 1989; 171: 6580-6585.
35. Hamilä H, Palva A, Paulin L, Arvidson S, Palva I. Secretory S complex of Bacillus subtilus: sequence analysis and identity to pyruvate dehydrogenase. J Bacteriol 1990; 172: 5052-5063.
36. Else AJ, Hough DW, Danson MJ. Cloning, sequencing, and expression of Trypanosoma brucei dihydrolipoamide dehydrogenase. Eur J Biochem 1993; 212: 423-429.
37. Fussey SPM, Guest JR, James OFW, Bassendine MF, Yeaman SJ. Identification and analysis of the major M2 autoantigens in primary biliary cirrhosis. Proc Natl Acad Sci USA 1988; 85: 8654-8658.
38. Fredriksen JH, Griffiths E, Grotterod EM et al. Characterization of high molecular weight components in Men B vaccine "Folkhelsa": an outer membrane vesicle vaccine against group B meningococcal disease. In: Conde-Glez CJ, Morse S, Rice P, F.S, Calderon E (eds) Pathobiology and immunobiology of Neisseriaceae. Cuernavaca, Mexico: Publications office of the Instituto Nacional de Salud Publica, 1994: 818-824.
39. Griffiths E, Sierra G, Holst J. Quality control of the Cuban and Norwegian serogroup B vaccines used in the Iceland study. In: Evans JS, Susan EY, Martin CJM, Feavers IM (eds) Neisseria 1994. Potters Bar, UK, NIBSC. 1994: 437.