Subcellular localization of boron in cultured melanoma cells by electron energy-loss spectroscopy of freeze-dried cryosections

Journal of Microscopy

Volumn 210, Issue 1, 2003, Pages 25-34

  Michel, Jean ^c   Sauerwein, W ^a   Wittig, A ^a   Balossier, G ^c   Zierold, K ^b  

^a UNIVERSITY OF DUISBURG ESSEN   (Germany)

^b MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^c NONE

Author keywords

Boron; Cryomethods; Detection limits; Digital filters; Electron energy loss spectroscopy (EELS); Human melanoma cells; Neutron capture therapy (NCT); Sodium borocaptate (BSH); Spectrum imaging

Indexed keywords

BANDPASS FILTERS; BORON; CELLS; CYTOLOGY; DISSOCIATION; ELECTRON EMISSION; ELECTRON ENERGY LEVELS; ELECTRON ENERGY LOSS SPECTROSCOPY; ELECTRON SCATTERING; ONCOLOGY;

CRYOMETHODS; DETECTION LIMITS; ELECTRON ENERGY-LOSS SPECTROSCOPIES; ELECTRON ENERGY-LOSS SPECTROSCOPY; HUMAN MELANOMA CELL; MELANOMA CELLS; NEUTRON CAPTURE THERAPY; SODIUM BOROCAPTATE (BSH); SPECTRUM IMAGING;

ENERGY DISSIPATION;

BOROCAPTATE;

ANALYTIC METHOD; CALIBRATION; CANCER CELL CULTURE; CELLULAR DISTRIBUTION; CONFERENCE PAPER; CONTROLLED STUDY; CULTURE MEDIUM; DRUG DISTRIBUTION; ELECTRON ENERGY LOSS SPECTROSCOPY; FREEZE DRYING; FROZEN SECTION; HUMAN; HUMAN CELL; MELANOMA CELL; NEUTRON CAPTURE THERAPY; PRIORITY JOURNAL;

ANGUILLIFORMES;

EID: 0344406073     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.2003.01172.x     Document Type: Conference Paper

References (41)

1. Autry, S.A., Gandour-Edwards, R., Boggan, J.E., Mayhew, D.E., Edwards, B.F., Fisher, D.A., Munn, R., Moraes, A. & Carbonell, R.G. (1997) Subcellular localisation of neutron capture elements using electron energy loss spectroscopic imaging. Advances in Neutron Capture Therapy, Vol. II Chemistry and Biology (ed. by B. Larson, J. Crawford & R. Weinreich), pp. 347-353. Elsevier, Amsterdam.
2. Balossier, G., Thomas, X., Michel, J., Wagner, D., Bonhomme, P., Puchelle, E., Ploton, D., Bonhomme, A. & Pinon, J.M. (1991) Parallel EELS elemental mapping in scanning transmission electron microscopy: use of the difference methods. Microsc. Microanal. Microstruct. 2, 531-546.
3. Barth, R.F., Soloway, A.H. & Brugger, R.M. (1996) Boron neutron capture therapy of brain tumors; Past history, current status, and future potential. Cancer Invest. 14, 534-550.
4. Bendayan, M., Barth, R.F.Gingras, D., Londoño, I., Robinson, P.T., Alam, F., Adams, D.M. & Mattiazi, L. (1989) Electron spectroscopic imaging for high-resolution immunocytochemistry: use of boronated protein A. J. Histochem. Cytochem. 37, 573-580.
5. Bevington, P.R. (1969) Data Reduction and Error Analysis for the Physical Sciences. McGraw-Hill Book Company, New York.
6. Bonnet, N., Michel, J., Wagner, D. & Balossier, G. (1992) Optimization of digital filters for the detection of trace elements in electron energy loss spectroscopy. I. Theory. Ultramicroscopy, 41, 105-114.
7. Diaz, A.Z., Coderre, J.A., Chanana, A.D. & Ma, R. (2000) Boron neutron capture therapy for malignant gliomas. Ann. Med. 32, 81-85.
8. Dorn, R.V. (1994) Boron neutron capture therapy (BNCT): a radiation oncology perspective. Int. J. Radiation Oncol. Biol. Phys. 28, 1189-1201.
9. Egerton, R.F. (1982a) A revised expression for signal/noise ratio in EELS. Ultramicroscopy, 9, 387-390.
10. Egerton, R.F. (1996) Electron Energy-Loss Spectroscopy in the Electron Microscope. Plenum Press, New York.
11. Egerton, R.F., Yang, Y.Y. & Cheng, S.G. (1993) Characterization and use of the Gatan 666 parallel-recording electron energy loss spectrometer. Ultramicroscopy, 48, 239-250.
12. Elhanati, G., Salomon, Y. & Bendel, P. (2001) Significant differences in the retention of the borocaptate monomer (BSH) and dimer (BSSB) in malignant cells. Cancer Lett. 172, 127-132.
13. Feng, J.L., Ho, R., Shao, Z. & Somlyo, A.P. (1999) Parallel electron energy-loss spectroscopy free from gain variations. Ultramicroscopy, 76, 221-231.
14. Hideghéty, K., Sauerwein, W., Haselberger, K., Grochulla, F., Fankhauser, H., Moss, R., Huiskamp, R., Gabel, D. & De Vries, J.M. (1999) Postoperative treatment of glioblastoma with BNCT at the Petten irradiation facility (EORTC Protocol 11961). Strahlenter Onkol. 175, 111-114.
15. Hunt, J.A. & Williams, D.B. (1991) Electron energy-loss spectrum-imaging. Ultramicroscopy, 38, 47-73.
16. International Atomic Energy Agency IAEA. (2001) Current Status of Neutron Capture Therapy. IAEA-TECDOC-1223. International Atomic Energy Agency, Vienna.
17. Isaacson, I. & Johnson, D. (1975) The microanalysis of light elements using transmitted energy-loss electrons. Ultramicroscopy, 1, 33-52.
18. Jeanguillaume, C. & Colliex, C. (1989) Spectrum-image: the next step in EELS digital acquisition and processing. Ultramicroscopy, 28, 252.
19. Kiger, W.S., Palmer, M.R., Riley, K.J., Zamenhof, R.G. & Busse, P.M. (2001) A pharmacokinetic model for the concentration of 10B in blood after boronophenylalanine-fructose administration in humans. Radiat. Res. 55, 611-618.
20. Lavergne, J.L., Martin, J.M. & Belin, M. (1992) Interactive electron energy-loss elemental mapping by the 'Imaging-Spectrum' method. Microsc. Microanal. Microstruct. 3, 517-528.
21. Leapman, R.D. & Swyt, C.R. (1988) Separation of overlapping core edges in electron energy loss spectra by multiple-least-squares fitting. Ultramicroscopy, 26, 393-404.
22. Leapman, R.D., Hunt, J.A., Buchanan, R.A. & Andrews, S.B. (1993) Measurement of low calcium concentrations in cryosectioned cells by parallel-EELS mapping. Ultramicroscopy, 49, 225-235.
23. Leapman, R.D. & Ornberg, R.L. (1988) Quantitative electron energy loss spectroscopy in biology. Ultramicroscopy, 24, 251-268.
24. Michel, J. & Bonnet, N. (2001) Optimization of digital filters for the detection of trace elements in EELS. Gaussian, homomorphic and adaptive filters. Ultramicroscopy, 88, 231-242.
25. Michel, J., Bonnet, N., Wagner, D., Balossier, G. & Bonhomme, P. (1993) Optimization of digital filters for the detection of trace elements in electron energy loss spectroscopy II Experiments. Ultramicroscopy, 48, 121-132.
26. Mory, C., Kohl, H., Tencé, M. & Colliex, C. (1991) Experimental investigation of the ultimate EELS spatial resolution. Ultramicroscopy, 37, 191-201.
27. Otersen, B., Sierralta, W.D., Haritz, D., Grochulla, F., Bergmann, M. & Gabel, D. (1997) Cellular uptake mechanism of BSH in tumor tissue of glioma patients. Advances in Neutron Capture Therapy, Vol. II Chemistry and Biology (ed. by B. Larson, J. Crawford & R. Weinreich), pp. 169-174. Elsevier, Amsterdam.
28. Pollmann, D., Broekaert, J.A.C., Leis, F., Tschöpel, P. & Tölg, G. (1993) Determination of boron in biological tissues by inductively coupled plasma optical emission spectrometry (ICP-OES). Fresenius, 117, 1-5.
29. Probst, T.U. (1999) Methods for boron analysis in boron neutron capture therapy. A review. Fresenius J. Anal. Chem. 364, 391-403.
30. Sauerwein, W. (1993) Principles and history of neutron capture therapy. Strahlenther. Onkol. 1, 1-6.
31. Sauerwein, W., Hideghéty, K., Rassow, J., De Vries, J.M., Götz, C., Paquis, P., Grochulla, F., Wolbers, J.G., Haselberger, K., and the EORTC BNCT Study Group (2001) First clinical results from the EORTC phase I trial 'Postoperative treatment of glioblastoma with BNCT at the Pettten irradiation facility', IAEA-TecdoC-1223. Current Status of Neutron Capture Therapy, pp. 250-256. International Atomic Energy Agency, Vienna.
32. Shuman, H. & Somlyo, A.P. (1987) Electron energy loss analysis of near-trace-element concentrations of calcium. Ultramicroscopy, 21, 23-32.
33. Smith, D.R., Chandra, S., Barth, R.F., Yang, W., Joel, D.D. & Coderre, J.A. (2001) Quantitative imaging and microlocalization of boron 10 in brain tumors and infiltrating tumor cells by SIMS ion microscopy: Relevance to neutron capture therapy. Cancer Res. 61, 8179-8187.
34. Solares, G.R. & Zamenhof, R.G. (1995) A novel approach to the microdosimetry of neutron capture therapy. Part I. High-resolution quantitative autoradiography applied to microdosimetrey in neutron capture therapy. Radiat. Res. 144, 50-58.
35. Soloway, A.H., Hatanaka, H. & Davis, M.A. (1967) Penetration of brain and brain tumor. VII. Tumor binding sulfhydryl boron compounds. J. Med. Chem. 10, 714-717.
36. Wittig, A., Hidegehty, K., Paquis, Ph, Heimans, J., Vos, M., Goetz, C., Haselberger, K., Grochulla, F., Moss, R., Morissey, J., Bourhis-Martin, E., Rassow, J., Stecher-Rasmussen, F., Turowski, B., Wiestler, M., De Vries, M.J., Fankhauser, H., Gabel, D. & Sauerwein, W. (2002) Current Clinical Results of the EORTC-Study 11961. Research and Development in Neutron Capture Therapy, Vol. S., pp. 1117-1122. Monduzzi Editore, Bologna.
37. Wittig, A., Sauerwein, W., Pöller, F., Fuhrmann, C., Hideghéty, K. & Streffer, C. (1998) Evaluation of boron neutron capture effects in cell culture using sulforhodamine-B assay and a colony assay. Int. J. Radiat. Biol. 73, 679-690.
38. Zhu, Y., Egerton, R.F. & Malac, M. (2001) Concentration limits for the measurement of boron by electron energy-loss spectroscopy and electron-spectroscopic imaging. Ultramicroscopy, 87, 135-145.
39. Zierold, K. (1988) X-ray microanalysis of freeze-dried and frozen-hydrated cryosections. Electron Microsc. Techn. 9, 65-82.
40. Zierold, K. (1991) Cryofixation methods for ion localization in cells by electron probe microanalysis: a review. J. Microsc. 161, 357-366.
41. Zierold, K., Hentschel, H., Wehner, F. & Wessing, A. (1994) Electron probe X-ray microanalysis of epithelial cells: aspects of cryofixation. Scanning Microsc. 8, 117-127.