FT-IR study of heterologous protein expression in recombinant Escherichia coli strains

Biochimica et Biophysica Acta - General Subjects

Volumn 1624, Issue 1-3, 2003, Pages 6-10

  Ami, Diletta ^a   Bonecchi, Loredana ^b   Calì, Simona ^b   Orsini, Gaetano ^b   Tonon, Giancarlo ^b   Doglia, Silvia Maria ^a  

^a UNIVERSITY OF MILANO BICOCCA   (Italy)

^b Biotechnology Division   (Italy)

Author keywords

Escherichia coli; Expression of heterologous protein; FT IR microspectroscopy; Inclusion body; Interferon alpha

Indexed keywords

BACTERIAL PROTEIN;

ARTICLE; CELL INCLUSION; CONTROLLED STUDY; ESCHERICHIA COLI; INFRARED SPECTROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS;

ESCHERICHIA COLI;

EID: 0344395589     PISSN: 03044165     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbagen.2003.09.008     Document Type: Article

References (15)

1. Tatcher D.R., Hitchcock A. Protein folding in biotechnology. Pain R.H. Mechanism of Protein Folding. 1994;229-261 IRL Press, Oxford.
2. Mc'Govern A.C., Ernill R., Kara B.V., Kell D.B., Goodacre R. Rapid analysis of the expression of heterologous proteins in Escherichia coli using pyrolysis mass spectrometry and Fourier transform infrared spectroscopy with chemometrics: application to alpha-2-interferon production. J. Biotechnol. 72:1999;157-168.
3. Naumann D., Helm D., Labischinski H. Microbiological characterizations by Fourier transform infrared spectroscopy. Nature. 35:1991;81-82.
4. Kirschner C., Maquelin K., Pina P., Ngo Ti N.A., Choo-Smith L.P., Sockalingum G.D., Sandt C., Ami D., Orsini F., Doglia S.M., Allouch P., Manfait M., Puppels G.J., Naumann D. Classification and identification of Enterococci: a comparative phenotypic, genotypic, and vibrational spectroscopic study. J. Clin. Microbiol. 39:2001;1763-1770.
5. Goodacre R., Timmins E.M., Rooney P.J., Rowland J.J., Kell D.B. Rapid identification of Streptococcus and Enterococcus species using diffuse reflectance-absorbance Fourier transform infrared spectroscopy and artificial neural networks. FEMS Microbiol. Lett. 140:1996;233-239.
6. Orsini F., Ami D., Villa A.M., Sala G., Bellotti M.G., Doglia S.M. FT-IR microspectroscopy for microbiological studies. J. Microbiol. Methods. 42:2000;17-27.
7. Seshadri S., Khurana R., Fink A.L. Fourier transform infrared spectroscopy in analysis of protein deposits. Methods Enzymol. 309:1999;559-576.
8. Choo-Smith L.P., Maquelin K., VanVreeswijk T., Bruining H.A., Puppels G.J., Ngo Ti N.A., Kirschner C., Naumann D., Ami D., Villa A.M., Orsini F., Doglia S.M., Lamfarraj H., Sockalingum G.D., Manfait M., Allouch P., Endtz H.P. Investigating microbial (micro) colony heterogeneity by vibrational spectroscopy. Appl. Environ. Microbiol. 67:2001;1461-1469.
9. M. Vanoni, P. Tortora, G. Tonon, G. Taylor, G. Orsini, Novel soluble endoprotease for the in vitro processing of recombinant proteins, International patent application (2001) PCT/EPO1/13257.
10. Kane J.F. Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli. Curr. Opin. Biotechnol. 6:1995;494-500.
11. Arrondo J.L.R., Goni F.M. Structure and dynamics of membrane proteins as studied by infrared spectroscopy. Prog. Biophys. Mol. Biol. 72:1999;367-405.
12. Susi H., Byler D.M. Resolution-enhanced Fourier transform infrared spectroscopy of enzymes. Methods Enzymol. 130:1986;291-311.
13. Oberg K., Chrunyk B.A., Wetzel R., Fink A.L. Nativelike secondary structure in interleukin-1β inclusion bodies by attenuated total reflectance FT-IR. Biochemistry. 33:1994;2628-2634.
14. Dong A., Matsuura J., Allison S.D., Chrisman E., Manning M.C., Carpenter J.F. Infrared and circular dichroism spectroscopic characterization of structural differences between β-lactoglobulin A and B. Biochemistry. 35:1996;1450-1457.
15. Dong A., Huang P., Caughey W.S. Protein secondary structures in water from second derivative amide I infrared spectra. Biochemistry. 29:1990;3303-3308.