Functional Domains of the RhlR Transcriptional Regulator of Pseudomonas aeruginosa

Journal of Bacteriology

Volumn 185, Issue 24, 2003, Pages 7129-7139

  Lamb, Janet R ^a   Patel, Hetal ^a   Montminy, Timothy ^a   Wagner, Victoria E ^a   Iglewski, Barbara H ^a,b  

^a UNIVERSITY OF ROCHESTER   (United States)

^b UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; LEXA PROTEIN; N BUTYRYL HOMOSERINE LACTONE; PROTEIN RHLR; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; CARBOXY TERMINAL SEQUENCE; CELL DENSITY; ESCHERICHIA COLI; GENE ACTIVATION; GENE DELETION; GENE EXPRESSION REGULATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN INTERACTION; PSEUDOMONAS AERUGINOSA; TRANSCRIPTION REGULATION; WILD TYPE;

4-BUTYROLACTONE; BACTERIAL PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, TERTIARY; PSEUDOMONAS AERUGINOSA; SERINE ENDOPEPTIDASES; TRANS-ACTIVATION (GENETICS);

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0344235401     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.24.7129-7139.2003     Document Type: Article

References (35)

1. Brint, J. M., and D. E. Ohman. 1995. Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the control of RhlR-RhlI, another set of regulators in strain PAO1 with homology to the autoinducer-responsive LuxR-LuxI family. J. Bacteriol. 177:7155-7163.
2. Choi, S. H., and E. P. Greenberg. 1992. Genetic evidence for multimerization of LuxR, the transcriptional activator of Vibrio fischeri luminescence. Mol. Marine Biol. Biotechnol. 1:408-413.
3. Choi, S. H., and E. P. Greenberg. 1991. The C-terminal region of the Vibrio fischeri LuxR protein contains an inducer-independent lux gene activating domain. Proc. Natl. Acad. Sci. USA 88:11115-11119.
4. Chugani, S. A., M. Whiteley, K. M. Lee, D. D'Argenio, C. Manoil, and E. P. Greenberg. 2001. QscR, a modulator of quorum-sensing signal synthesis and virulence in Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA 98:2752-2757.
5. Daines, D. A., and R. P. Silver. 2000. Evidence for multimerization of neu proteins involved in polysialic acid synthesis in Escherichia coli K1 using improved LexA-based vectors. J. Bacteriol. 182:5267-5270.
6. Devine, J. H., G. S. Shadel, and T. O. Baldwin. 1989. Identification of the operator of the lux regulon from the Vibrio fischeri strain ATCC 7744. Proc. Natl. Acad. Sci. USA 86:5688-5692.
7. Dmitrova, M., G. Younes-Cauet, P. Oertel-Buchheit, D. Porte, M. Schnarr, and M. Granger-Schnarr. 1998. A new LexA-based genetic system for monitoring and analyzing protein heterodimerization in Escherichia coli. Mol. Gen. Genet. 257:205-212.
8. Govan, J., and G. Harris. 1986. Pseudomonas aeruginosa and cystic fibrosis: unusual bacterial adaptation and pathogenesis. Microbiol. Sci. 3:302-308.
9. Gray, K. M., and J. R. Garey. 2001. The evolution of bacterial LuxI and LuxR quorum sensing regulators. Microbiology 147:2379-2387.
10. Hanzelka, B. L., and E. P. Greenberg. 1995. Evidence that the N-terminal region of the Vibrio fischeri LuxR protein constitutes an autoinducer-binding domain. J. Bacteriol. 177:815-817.
11. Hardalo, C., and S. C. Edberg. 1997. Pseudomonas aeruginosa: assessment of risk from drinking water. Crit. Rev. Microbiol. 23:47-75.
12. E). Proc. Natl. Acad. Sci. USA 92:7941-7945.
13. Hoang, T. T., A. J. Kutchma, A. Becher, and H. P. Schweizer. 2000. Integration-proficient plasmids for Pseudomonas aeruginosa: site-specific integration and use for engineering of reporter and expression strains. Plasmid 43:59-72.
14. Howorka, S., and H. Bayley. 1998. Improved protocol for high-throughput cysteine scanning mutagenesis. BioTechniques 25:764-766.
15. Kiratisin, P., K. D. Tucker, and L. Passador. 2002. LasR, a transcriptional activator of Pseudomonas aeruginosa virulence genes, functions as a multimer. J. Bacteriol. 184:4912-4919.
16. Kline, T., J. Bowman, B. H. Iglewski, T. de Kievit, Y. Kakai, and L. Passador. 1999. Novel synthetic analogs of the Pseudomonas autoinducer. Bioorg. Med. Chem. Lett. 9:3447-3452.
17. Ledgham, F., I. Ventre, C. Soscia, M. Foglino, J. N. Sturgis, and A. Lazdunski. 2003. Interactions of the quorum sensing regulator QscR: interaction with itself and the other regulators of Pseudomonas aeruginosa LasR and RhlR. Mol. Microbiol. 48:199-210.
18. Luo, Z. Q., and S. K. Farrand. 1999. Signal-dependent DNA binding and functional domains of the quorum-sensing activator TraR as identified by repressor activity. Proc. Natl. Acad. Sci. USA 96:9009-9014.
19. Luo, Z. Q., A. J. Smyth, P. Gao, Y. Qin, and S. K. Farrand. 2003. Mutational analysis of TraR: correlating function with molecular structure of a quorum-sensing transcriptional activator. J. Biol. Chem. 4:4.
20. Miller, J. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
21. Ohman, D. E., S. J. Cryz, and B. H. Iglewski. 1980. Isolation and characterization of a Pseudomonas aeruginosa PAO mutant that produces altered elastase. J. Bacteriol. 142:836-842.
22. Pearson, J. P., E. C. Pesci, and B. H. Iglewski. 1997. Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes. J. Bacteriol. 179:5756-5767.
23. Pesci, E., and B. Iglewski. 1999. Quorum sensing in Pseudomonas aeruginosa, p. 147-155. In G. Dunny and S. Winans (ed.), Cell-cell signaling in bacteria. ASM Press, Washington, D.C.
24. Pesci, E., J. Pearson, P. Seed, and B. Iglewski. 1997. Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa. J. Bacteriol. 179:3127-3132.
25. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
26. Schuster, M., C. P. Lostroh, T. Ogi, and E. P. Greenberg. 2003. Identification, timing, and signal specificity of Pseudomonas aeruginosa quorum-controlled genes: a transcriptome analysis. J. Bacteriol. 185:2066-2079.
27. Seed, P., L. Passador, and B. Iglewski. 1995. Activation of the Pseudomonas aeruginosa lasI gene by LasR and the Pseudomonas autoinducer PAI: an autoinduction regulatory hierarchy. J. Bacteriol. 177:654-659.
28. Smith, A. W., and B. H. Iglewski. 1989. Transformation of Pseudomonas aeruginosa by electroporation. Nucleic Acids Res. 17:10509.
29. Stevens, A., and E. P. Greenberg. 1999. Transcriptional activation by LasR, p. 231-242. In G. Dunny and S. Winans (ed.), Cell-cell signaling in bacteria. ASM Press, Washington, D.C.
30. Vannini, A., C. Volpari, C. Gargioli, E. Muraglia, R. Cortese, R. De Francesco, P. Neddermann, and S. D. Marco. 2002. The crystal structure of the quorum sensing protein TraR bound to its autoinducer and target DNA. EMBO J. 21:4393-4401.
31. Ventre, I., F. Ledgham, V. Prima, A. Lazdunski, M. Foglino, and J. N. Sturgis. 2003. Dimerization of the quorum sensing regulator RhlR: development of a method using EGFP fluorescence anisotropy. Mol. Microbiol. 48:187-198.
32. Wagner, V. E., D. Bushnell, L. Passador, A. I. Brooks, and B. H. Iglewski. 2003. Microarray analysis of Pseudomonas aeruginosa quorum-sensing regulons: effects of growth phase and environment. J. Bacteriol. 185:2080-2095.
33. Welch, M., D. E. Todd, N. A. Whitehead, S. J. McGowan, B. W. Bycroft, and G. P. Salmond. 2000. N-acyl homoserine lactone binding to the CarR receptor determines quorum-sensing specificity in Erwinia. EMBO J. 19:631-641.
34. Zhang, R. G., T. Pappas, J. L. Brace, P. C. Miller, T. Oulmassov, J. M. Molyneaux, J. C. Anderson, J. K. Bashkin, S. C. Winans, and A. Joachimiak. 2002. Structure of a bacterial quorum-sensing transcription factor complexed with pheromone and DNA. Nature 417:971-974.
35. Zhu, J., and S. Winans. 2001. The quorum-sensing transcriptional regulator TraR requires its cognate signaling ligand for protein folding, protease resistance, and dimerization. Proc. Natl. Acad. Sci. USA 98:1507-1512.