The accessibility of iron at the active site of recombinant human phenylalanine hydroxylase to water as studied by 1H NMR paramagnetic relaxation. Effect of L-Phe and comparison with the rat enzyme

Journal of Biological Chemistry

Volumn 274, Issue 10, 1999, Pages 6280-6284

  Olafsdottir, Sigridur ^a,b   Martínez, Aurora ^a  

^a UNIVERSITY OF ICELAND   (Iceland)

^b UNIVERSITY OF BERGEN   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

IRON; PHENYLALANINE 4 MONOOXYGENASE; RECOMBINANT PHENYLALANINE 4 MONOOXYGENASE; UNCLASSIFIED DRUG; WATER;

ANIMAL CELL; ARTICLE; ELECTRON SPIN RESONANCE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME STRUCTURE; HUMAN; HUMAN CELL; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; RAT; TEMPERATURE MEASUREMENT;

ANIMALS; HUMANS; IRON; MAGNETIC RESONANCE SPECTROSCOPY; PHENYLALANINE; PHENYLALANINE HYDROXYLASE; RATS; RECOMBINANT PROTEINS; WATER;

EID: 0344178151     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.274.10.6280     Document Type: Article

References (33)

1. Kaufman, S. (1993) Adv. Enzymol. Relat. Areas Mot. Biol. 67, 77-264
2. Kappock, T. J., and Caradonna, J. P. (1996) Chem. Rev. 96, 2659-2756
3. Kaufman, S., and Fisher, D. (1974) in Molecular Mechanisms of Oxygen Activation (Hayaishi, O., ed) pp. 285-369, Academic Press, New York
4. Wallick, D. E., Bloom, L. M., Gaffney, B. J., and Benkovic, S. J. (1984) Biochemistry 23, 1295-1302
5. Bloom, L. M., Benkovic, S. J., and Gaffney, B. J. (1986) Biochemistry 25, 4204-4210
6. Scriver, C. R., Kaufman, S., Eisensmith, R. C., and Woo, S. L. C. (1995) in The Metabolic and Molecular Bases of Inherited Disease (Scriver, C. R., Beaudet, A. L., Sly, W. S., and Valle, D., eds) 7th Ed., pp. 1015-1075, McGraw-Hill, New York
7. Martínez, A., Knappskog, P. M., Olafsdottir, S., Døskeland, A. P., Eiken, H. G., Svebak, R. M., Bozzini, ML., Apold, J., and Flatmark, T. (1995) Biochem. J. 306, 589-597
8. Knappskog, P. M., Flatmark, T., Aarden, J. M., Haavik, J., and Martínez, A. (1996) Bur. J. Biochem. 242, 813-821
9. Chehin, R., Thorolfsson, M., Knappskog, P. M., Martínez, A., Flatmark, T., Arrondo, J. L., and Muga, A. (1998) FEBS Lett. 422, 225-230
10. Døskeland, A. P., Martínez, A., Knappskog, P. M., and Flatmark, T. (1996) Biochem. J. 313, 409-414
11. Ledley, F. D., Grenett, H. E., and Woo, S. L. C. (1987) J. Biol. Chem. 262, 2228-2233
12. Erlandsen, H., Fusetti, F., Martínez, A., Hough, E., Flatmark, T., and Stevens, R. C. (1997) Nat. Struct. Biol. 4, 995-1000
13. Fusetti, F., Erlandsen, H., Flatmark, T., and Stevens, R. C. (1998) J Biol. Chem. 273, 16962-16967
14. Kwok, S. C. M., Ledley, F. D., DiLella, A. G., Robson, K. J. H., and Woo. S. L. C. (1985) Biochemistry 24, 556-561
15. Knappskog, P. M., and Martínez, A. (1997) FEBS Lett. 409, 7-11
16. Citron, B. A., Davis, M. D., and Kaufman, S. (1992) Protein Expression Purif. 3, 93-100
17. Kowlessur, D., Citron, B. A., and Kaufman. S. (1996) Arch. Biochem. Biophys. 333, 85-95
18. Martínez, A., Olafsdottir, S., and Flatmark, T. (1993) Eur. J. Biochem. 211, 259-266
19. Shiman, R., Gray, D. W., and Pater, A. (1979) J. Biol. Chem. 254, 11300-11306
20. Gottschall, D. W., Dietrich, R. F., Benkovic, S. J., and Shiman, R. (1982) J. Biol. Chem. 257, 845-849
21. Blair, D., and Diehl, H. (1961) Talanta 7, 163-174
22. Solomon, I. (1955) Phys. Rev. 99, 559-565
23. Bloembergen, N. (1957) J. Chem. Phys. 27, 572-573
24. Banci, L., Bertini, I., and Luchinat, C. (1991) Nuclear and Electron Relaxation. The Magnetic Nucleus-Unpaired Electron Coupling in Solution. VCH Verlagsgesellschaft mbH, Weinheim, Germany
25. Dwek, R. A. (1975) in Nuclear Magnetic Resonance (N. M. R.) in Biochemistry. Applications to Enzyme Systems, pp. 247-284, Oxford University Press, Oxford, UK
26. Martínez, A., Andersson, K. K., Haavik, J., and Flatmark, T. (1991) Eur. J. Biochem. 198, 675-682
27. Mildvan, A. S., Granot, J., Smith, G. M., and Liebman, M. N. (1980) in Methods for Determining Metal Ion Environments in Proteins. Structure and Function of Metalloproteins (Darnall, D. W., and Wilkins, R. G., eds) pp. 211-236, Elsevier North-Holland, Inc., New York
28. Loeb, K. E., Westre, T. E., Kappock, T. J., Mitic, N., Glasfeld, E., Caradonna, J. P., Hedman, B., Hodgson, K. O., and Solomon, E. I. (1997) J. Am. Chem. Soc. 119, 1901-1915
29. Burton, D. R., Forsen, S., Karlstrom, G., and Dwek, R. A. (1979) Prog. Nuclear Magn. Reson. Spectrose. 13, 1-45
30. Jarori, G. K., Ray, B. D., and Rao, B. D. N. (1989) Biochemistry 28, 9343-9350
31. Hershberg, R. D., and Chance, B. (1975) Biochemistry 14, 3885-3891
32. Que, L., Jr., Lipscomb, J. D., Münck, E., and Wood, J. M. (1977) Biochim. Biophys. Acta 485, 60-74
33. Martínez, A., Haavik, J., and Flatmark, T. (1990) Eur. J. Biochem. 193, 211-219