The metG gene of Aspergillus nidulans encoding cystathionine β-lyase: Cloning and analysis

Current Genetics

Volumn 35, Issue 6, 1999, Pages 638-646

  Sieńko, Marzena ^a   Paszewski, Andrzej ^a  

^a INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS   (Poland)

Author keywords

Aspergillus nidulans; Cystathionine lyase, EC 4.4.1.8 ; metG gene

Indexed keywords

BINDING PROTEIN; COMPLEMENTARY DNA; CYSTATHIONINE; LYASE; METHIONINE; PYRIDOXAL 5 PHOSPHATE; SULFUR;

AMINO ACID SYNTHESIS; ARTICLE; ASPERGILLUS NIDULANS; CULTURE MEDIUM; DNA POLYMORPHISM; ESCHERICHIA COLI; FUNGAL GENETICS; GENETIC COMPLEMENTATION; INTRON; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; PRIORITY JOURNAL; SILENT GENE; TRANSCRIPTION REGULATION;

ASPERGILLUS; ASPERGILLUS NIDULANS; BACTERIA (MICROORGANISMS); EMERICELLA NIDULANS; ESCHERICHIA COLI; FUNGI; MYXOGASTRIA;

EID: 0344131963     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002940050463     Document Type: Article

References (39)

1. Alexander FW, Sandmeier E, Mehta PK, Christen Ph (1994) Evolutionary relationships among pyridoxal-5′-phosphate-dependent enzymes. Region-specific α, β and γ families. Eur J Biochem 219:953-960
2. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403-410
3. Bairoch A, Bucher P, Hofmann K (1995) The PROSITE database, its status in 1995. Nucleic Acids Res 24:189-196
4. Ballance DJ, Turner G (1985) Development of a high-frequency transforming vector for Aspergillus nidulans. Gene 36:321-331
5. Belfaiza J, Parsot C, Martel A, Bouthier de la Tour C, Margarita D, Cohen GN, Saint-Girons I (1986) Evolution in biosynthetics pathways: two enzymes catalyzing consecutive steps in methionine biosynthesis originate from a common ancestor and possess a similar regulatory region. Proc Natl Acad Sci USA 83:867-871
6. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
7. Brody H, Griffith J, Cuticchia AJ, Arnold J, Timberlake WE (1991) Chromosome-specific recombinant DNA libraries from the fungus Aspergillus nidulans. Nucleic Acids Res 19:3105-3109
8. Cherest H, Thomas D, Surdin-Kerjan Y (1993) Cysteine biosynthesis in Saccharomyces cerevisiae occurs through the transsulfuration pathway which has been built up by enzyme recruitment. J Bacteriol 175:5366-5374
9. Chomczyński P (1993) TRI Reagent-RNA, DNA, protein isolation reagent. Manufacturer protocol, Molecular Research Center, Inc. Cincinnati, Ohio
10. Clarke DL, Newbert RW, Turner G (1997) Cloning and characterisation of the adenosyl phosphosulphate kinase gene from Aspergillus nidulans. Curr Genet 32:408-412
11. Clausen T, Huber R, Laber B, Pohlenz H-D, Messerschmidt A (1996) Crystal structure of the pyridoxal-5′-phosphate-dependent cystathionine β-lyase from Escherichia coli at 1.83 Å. J Mol Biol 262:202-224
12. Clutterbuck AJ (1994) Linkage map and locus list. In: Martinelli SD, Kinghorn JR (eds) Aspergillus: 50 years on. Elsevier, Amsterdam, pp 791-824
13. Coglievina M, Klima R, Bertani I, Delneri D, Zaccaria P, Bruschi CV (1997) Sequencing of a 40.5-kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae. Yeast 13:55-64
14. Cove DJ (1966) The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochem Biophys Acta 113:51-56
15. Eki T, Naitou M, Hagiwara H, Ozawa M, Sasanuma SI, Sasanuma M, Tsuchiya Y, Shibata T, Hanaoka F, Murakami Y (1996) Analysis of a 36.2-kb DNA sequence including the right telomere of chromosome VI from Saccharomyces cerevisiae. Yeast 12:149-167
16. Fidel S, Doonan JH, Morris NR (1988) Aspergillus nidulans contains a single actin gene which has unique intron locations and encodes γ-actin. Gene 70:283-293
17. Gajewski W, Litwińska J (1968) Methionine loci and their suppressors in Aspergillus nidulans. Mol Gen Genet 102:210-220
18. Gems DH, Clutterbuck AJ (1993) Co-transformation with autonomously replicating helper plasmids facilitates gene cloning from an Aspergillus nidulans gene library. Curr Genet 24:520-524
19. Gems D, Johnstone IL, Clutterbuck AJ (1991) An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency. Gene 98:61-67
20. Gentry-Weeks CR, Keith JM, Thompson J (1993) Toxicity of Bordetella avium β-cystathionase toward MC3T3-E1 osteogenic cells. J Biol Chem 268:7298-7314
21. Hata Y, Tsuchiya K, Kitamoto K, Gomi K, Kumagai C, Tamura G, Hara S (1991) Nucleotide sequence and expression of the glucoamylase-encoding gene (glaA) from Aspergillus oryzae. Gene 108:145-150
22. Higgins DG, Sharp PM (1988) CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73:237-244
23. Kimura M (1968) Evolutionary rate at the molecular level. Nature 217:624-626
24. Kozak M (1991) Structural features in eukaryotic mRNAs that modulate the initiation of translation. J Biol Chem 266:19867-19870
25. Kunst F, et al (1997) The complete genome sequence of the grampositive bacterium Bacillus subtilis. Nature 390:249-256
26. Lloyd AT, Sharp PM (1991) Codon usage in Aspergillus nidulans. Mol Gen Genet 230:288-294
27. Martel A, Bouthier de la Tour C, Le Goffic F (1987) Pyridoxal 5′-phosphate binding site of Escherichia coli β-cystathionase and cystathionine γ-synthase: comparison of their sequences. Biochem Biophys Res Commun 147:565-571
28. Natorff R, Balińska M, Paszewski A (1993) At least four regulatory genes control sulphur metabolite repression in Aspergillus nidulans. Mol Gen Genet 238:185-192
29. Paszewski A, Grabski J (1973) Studies on β-cystathionase and O-acetylhomoserine sulfhydrylase as the enzymes of alternative methionine biosynthetic pathways in Aspergillus nidulans. Acta Biochim Polon 20:159-168
30. Paszewski A, Grabski J (1974) Regulation of S-amino acid biosynthesis in Aspergillus nidulans. Mol Gen Genet 132:307-320
31. Paszewski A, Grabski J (1975) Enzymatic lesions in methionine mutants of Aspergillus nidulans: role and regulation of an alternative pathway for cysteine and methionine synthesis. J Bacteriol 124:893-904
32. Ravanel S, Ruffet M-L, Droux M, Douce R (1995) Cloning of an Arabidopsis thaliana cDNA encoding cystathionine β-lyase by functional complementation in E. coli. Plant Mol Biol 29:875-882
33. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
34. Sieńsko M, Topczewski J, Paszewski A (1998) Structure and regulation of cysD, the homocysteine synthase gene of Aspergillus nidulans. Curr Genet 33:136-144
35. Topczewski J, Sieńko M, Paszewski A (1997) Cloning and characterization of the Aspergillus nidulans cysB gene encoding cysteine synthase. Curr Genet 31:348-356
36. Unkles SE (1992) Gene organization in filamentous fungi. In: Kinghorn JR, Turner G (eds) Applied molecular genetics of filamentous fungi. University Press, Cambridge, pp 28-53
37. Yamagata S, Paszewski A, Lewandowska I (1990) Purification and properties of O-acetyl-L-homoserine sulfhydrylase from Aspergillus nidulans. J Gen Appl Microbiol 36:137-141
38. Yelton MM, Hamer JE, Timberlake WE (1985) Transformation of Aspergillus nidulans by using a trpC plasmid. Proc Natl Acad Sci USA 81:1470-1474
39. Yonaha K, Nishie M, Aibara S (1992) The primary structure of ω-amino acid: pyruvate aminotransferase. J Biol Chem 267:12506-12510