Commitment to apoptosis by ceramides depends on mitochondrial respiratory function, cytochrome c release and caspase-3 activation in Hep-G2 cells

Molecular and Cellular Biochemistry

Volumn 254, Issue 1-2, 2003, Pages 203-210

  Gentil, Benoit ^a   Grimot, Fabrice ^a   Riva, Catherine ^a  

^a INSERM   (France)

Author keywords

Apoptosis; Caspases; Ceramides; Cytochrome c; Necrosis; ROS

Indexed keywords

ADENOSINE TRIPHOSPHATE; CASPASE 3; CERAMIDE; CYTOCHROME C; REACTIVE OXYGEN METABOLITE;

APOPTOSIS; ARTICLE; CELL ACTIVITY; CELL DEATH; CELL STRUCTURE; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; DNA CONTENT; ENZYME ACTIVATION; ENZYME RELEASE; FLOW CYTOMETRY; GEL ELECTROPHORESIS; GIEMSA STAIN; HEPATOMA CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; INCUBATION TIME; MITOCHONDRIAL RESPIRATION; PHENOTYPE; PURINE SYNTHESIS; RESPIRATORY CHAIN; TIME;

APOPTOSIS; CASPASE 3; CASPASES; CELL LINE; CELL LINE, TUMOR; CELL NUCLEUS; CELL SURVIVAL; CERAMIDES; COLORING AGENTS; CYTOCHROMES C; DNA; DNA FRAGMENTATION; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTRON TRANSPORT; ELECTROPHORESIS, AGAR GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; FLOW CYTOMETRY; HUMANS; MICROSCOPY, FLUORESCENCE; MITOCHONDRIA; REACTIVE OXYGEN SPECIES; SPHINGOSINE; TETRAZOLIUM SALTS; THIAZOLES; TIME FACTORS;

EID: 0344034332     PISSN: 03008177     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1027359832177     Document Type: Article

References (30)

1. Hannun YA: The sphyngomyelin cycle and second messenger function of ceramide. J Biol Chem 269: 3125-3128, 1994
2. Jarvis WD, Grant S, Kolesnick RN: Ceramide and the induction of apoptosis. Clin Cancer Res 2: 1-6, 1996
3. Bose R, Verheij M, Haimovitz-Friedman A, Scotto K, Fuks Z, Kolesnick R: Ceramide synthetase mediates daunorubicin-induced apoptosis: An alternative mechanism for generating death signals. Cell 82: 405-441, 1995
4. Gudz TI, Tserng K-Y, Hoppel CL: Direct inhibition of mitochondrial respiratory chain complex III by cell-permeable ceramide. J Biol Chem 272: 24154-24158, 1997
5. Garcia-Ruiz C, Colell A, Mari M, Morales A, Fernadez-Checa J: Direct interaction of ceramide on the mitochondrial electron transport chain leads to generation of reactive oxygen species. J Biol Chem 272: 11369-11377, 1997
6. Quillet-Mary A, Jaffrézou JP, Mansat V, Bordier C, Naval J, Laurent G: Implication of mitochondrial hydrogen peroxide generation in ceramide-induced apotosis. J Biol Chem 272: 21388-21395, 1997
7. Arora AS, Jones BJ, Patel TC, Bronk SF, Gores GJ: Ceramide induces hepatocytes cell death through disruption of mitochondrial function in the rat. Hepatology 25: 957-963, 1997
8. Darzynkiewicz Z, Juan G, Li X, Gorczyca W, Murakami T, Traganos F: Cytometry in cell necrobiology: Analysis of apoptosis and accidental cell death (necrosis). Cytometry 27: 1-20, 1997
9. Kroemer G, Reed J: Mitochondrial control of cell death. Nature Med 6: 513-519, 2000
10. Eguchi Y, Shimizu S, Tsujimoto Y: Intracellular ATP levels determine cell death fate by apoptosis or necrosis. Cancer Res 57: 1835-1840, 1997
11. Hirch T, Marchetti P, Suzin SA, Dallaporta B, Zamzami N, Marzo I, Geuskens M, Kroemer G: The apoptosis-necrosis paradox. Apoptogenic proteases activated after mitochondrial permeability transition determine the mode of cell death. Oncogene 15: 1573-1582, 1997
12. Goldstein JC, Waterhouse NJ, Juin P, Evan GI, Green DR: The coordinate release of cytochrome c during apoptosis is rapid, complete and kinetically invariant. Nature Cell Biol 2: 156-162, 2000
13. Cai J, Yang J, Jones DP: Mitochondrial control of apoptosis: The role of cytochrome c. Biochem Biophys Acta 1366: 139-149, 1998
14. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X: Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates and apoptotic protease cascade. Cell 91: 479-489, 1997
15. Appaix E, Minatchy MD, Riva-Lavieille C, Olivares O, Antonsson B, Saks V: Rapid spectrophotometic method for quantitation of cytochrome c release from isolated mitochondria or permeabilized cells revisited. Biochem Biophys Acta 1457: 175-181, 2000
16. Carmichael J, Mitchell JB, DeGraff WG, Gamson J, Gazdar AF, Johnson BE, Glatstein E, Minna JD: Chemosensitivity testing of human lung cancer cell lines using the MTT assay. Br J Cancer 57: 540-547, 1988
17. Searle J, Kerr JFR, Bishop CJ: Necrosis and apoptosis: Distinct modes of cell death with fundamentally different significance. Pathol Ann 17: 229-259, 1982
18. Hart LT, Morris NG, Bessin R, Leprince DJ, Todd WJ, Enright FM, Luther DG: Single-step technique for staining anaplasma marginale in bovine blood smears. Am J Vet Res 53: 1732-1733, 1992
19. Huang X, Frenkel K, Klein C, Costa M: Nickel induced oxidants in intact cultured mammalian cells as detected by dichlorofluorescein fluorescence. Pharmacol Toxicol 120: 29-36, 1993
20. Vento R, Giuliano M, Lauricella M, Carbillo M, Di Liberto D, Tesoriere G: Induction of programmed cell death in human retinoblastoma Y79 cells by C2-ceramide. Mol Cell Biochem 185: 7-15, 1998
21. Hung W-C, Chang H-C, Chuang L-Y: Activation of caspase-3-like proteases in apoptosis induced by sphyngosine and other long-chain bases in Hep3B hepatoma cells. Biochem J 338: 161-166, 1999
22. Singh NR: A simple method for accurate estimation of apoptotic cells. Exp Cell Res 256: 328-337, 2000
23. Puisieux A, Galvin K, Troalen F, Bressac B, Marcais C, Galun E, Ponchel F, Yakicier C, Ji J, Ozturk M: Retinoblastoma and p53 tumor suppressor genes in human hepatoma cell lines. Faseb J 7: 1407-1413, 1993
24. Ghafourifar P, Klein SD, Schucht O, Schenk U, Pruschy M, Rocha S, Richter C: Ceramide induces cytochrome c release from isolated mitochondria: importance of mitochondrial redox state. J Biol Chem 274: 6080-6084, 1999
25. Bernardi P, Scorrano L, Colonna R, Petronelli V, Di Lisa F: Mitochondria and cell death: Mechanistic aspect and methological issues. Eur J Biochem 264: 687-701, 1999
26. Venkataraman K, Futerman AH: Ceramide as a second messenger: Sticky solutions to sticky problems. Trends Cell Biol 10: 408-411, 2000
27. Verheij M, Bose R, Lin XH, Yao B, Jarvis WD, Grant S, Birrer MJ, Szabo E, Zon LI, Kyriakis JM, Haimovitz-Friedman A, Fuks Z, Kolesnick RN: Requirement for ceramide-initiated SAPK/JNK signaling in stress-induced apoptosis. Nature (Lond) 380: 75-79, 1996
28. XL in response to DNA damage. J Biol Chem 275: 322-327, 2000
29. Corda S, Laplace C, Vicaut E, Duranteau J: Rapid reactive oxygen species production by mitochondria in endothelial cells exposed to tumor necrosis factor-alpha is mediated by ceramide. Am J Resp Cell Mol Biol 24: 762-768, 2001
30. Marchenko ND, Zaika A, Moll UM: Death signal-induced localization of p53 protein to mitochondria. J Biol Chem 275: 16202-16212, 2000