Design of protein purification pathways: Application to the proteome of Haemophilus influenzae using heparin chromatography

Protein Expression and Purification

Volumn 14, Issue 1, 1998, Pages 113-119

  Fountoulakis, Michael ^a   Takács, Béla ^b  

^a Pharmaceutical Division   (Switzerland)

^b F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL METABOLISM; CHROMATOGRAPHY; DESIGN; HAEMOPHILUS INFLUENZAE; HEPARIN; MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEOME; SECONDARY METABOLITE; SEQUENCE ANALYSIS;

BACTERIA (MICROORGANISMS); HAEMOPHILUS INFLUENZAE;

EID: 0344015761     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1998.0943     Document Type: Article

References (18)

1. Wilkins M. R., Pasquali C., Appel R. D., Ou K., Golaz O., Sanchez J.-C., Yan J. X., Gooley A. A., Hughes G., Humphery-Smith I., Williams K. L., Hochstrasser D. F. From proteins to proteomes: Large scale protein identification by two-dimensional electrophoresis and amino acid analysis. Bio/Technology. 14:1996;61-65.
2. Righetti P. G., Bossi A. Isoelectric focusing in immobilized pH gradients: Recent analytical and preparative developments. Anal. Biochem. 247:1997;1-10.
3. Humphery-Smith I., Cordwell S. J., Blackstock W. P. Proteome research: Complementarity and limitations with respect to the RNA and DNA worlds. Electrophoresis. 18:1977;1217-1242.
4. Fountoulakis M., Langen H. Identification of proteins by matrix-assisted laser desorption ionization-mass spectrometry following in-gel digestion in low-salt, nonvolatile buffer and simplified peptide recovery. Anal. Biochem. 250:1997;153-156.
5. Golaz O., Wilkins M. R., Sanchez J.-C., Appel R. D., Hochstrasser D. F., Williams K. L. Identification of proteins by amino acid composition: An evaluation of the method. Electrophoresis. 17:1996;573-579.
6. Wheeler C. H., Berry S. L., Wilkins M. R., Corbett J. M., Ou K., Gooley A. A., Humphery-Smith I., Williams K. L., Dunn M. J. Characterization of proteins from two-dimensional electrophoresis gels by matrix-assisted laser desorption mass spectrometry and amino acid compositional analysis. Electrophoresis. 17:1996;580-587.
7. Fountoulakis M., Juranville J.-F., Berndt P. Large-scale identification of proteins ofHaemophilus influenzae. Electrophoresis. 18:1997;2968-2977.
8. Weiss M., Manneberg M., Juranville J.-F., Lahm H.-W., Fountoulakis M. Effect of the hydrolysis method on the determination of the amino acid composition of proteins. J. Chromatogr. 795:1998;263-275.
9. Fountoulakis M., Langen H., Evers S., Gray C., Takács B. Two-dimensional map ofHaemophilus influenzae. Electrophoresis. 18:1997;1193-1202.
10. Fountoulakis M., Takács B., Langen H. Two-dimensional map of basic proteins ofHaemophilus influenzae. Electrophoresis. 19:1998;761-766.
11. Fountoulakis M., Langen H., Gray C., Takács B. Enrichment and purification of proteins ofHaemophilus influenzae. J. Chromatogr. 806:1998;279-291.
12. Fleischmann R. D., Adams M. D., White O., Clayton R. A., Kirkness E. F., Kerlavage A. R., Bult C. J., Tomb J.-F., Dougherty B. A., Merrick J. M., Kenney K., Sutton G., FitzHugh W., Fields C., Gocayne J. D., Scott J., Shirley R., Liu L.-I., Glodek A., Kelley J. M., Weidman J. F., Phillips C. A., Spriggs T., Hedblom E., Cotton M. D., Utterback T R., Hanna M. C., Nguyen D. T., Saudek D. M., Brandon R. C., Fine L. D., Fritchman J. L., Fuhrmann J. L., Geoghagen N. S. M., Gnehm C. L., McDonald L. A., Small K. V., Fraser C. M., Smith H. O., Venter J. C. Haemophilus influenzae. Science. 269:1995;496-512.
13. Langen H., Gray C., Roeder D., Juranville J.-F., Takács B., Fountoulakis M. From genome to proteome: Protein map ofHaemophilus influenzae. Electrophoresis. 18:1997;1184-1192.
14. Bjellqvist B., Pasquali C., Ravier F., Sanchez J.-C., Hochstrasser D. F. A nonlinear wide-range immobilized pH gradient for two-dimensional electrophoresis and its definition in a relevant pH scale. Electrophoresis. 14:1993;1357-1365.
15. Langen H., Röder D., Juranville J.-F., Fountoulakis M. Effect of the protein application mode and the acrylamide concentration on the resolution of protein spots separated by two-dimensional gel electrophoresis. Electrophoresis. 18:1997;2085-2090.
16. Mann M., Wilm M. Error-tolerant identification of peptides in sequence databases by peptide sequence tags. Anal. Chem. 66:1994;4390-4399.
17. Pasquali C., Frutiger S., Wilkins M. R., Hughes G. J., Appel R. D., Bairoch A., Schaller D., Sanchez J. C., Hochstrasser D. F. Two-dimensional gel electrophoresis ofEscherichia coliEscherichia coli. Electrophoresis. 17:1996;547-555.
18. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.