Real-time imaging of individual fluorescent-protein color-coded metastatic colonies in vivo

Clinical and Experimental Metastasis

Volumn 20, Issue 7, 2003, Pages 633-638

  Yamamoto, Norio ^a,c   Yang, Meng ^a   Jiang, Ping ^a   Xu, Mingxu ^a   Tsuchiya, Hiroyuki ^c   Tomita, Katsuro ^c   Moossa, A R ^b   Hoffman, Robert M ^a,b  

^a ANTICANCER INC   (United States)

^b UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^c KANAZAWA UNIVERSITY   (Japan)

Author keywords

Experimental metastasis; Green fluorescent protein; HT 1080 fibrosarcoma; Lung external imaging; Red fluorescent protein

Indexed keywords

GREEN FLUORESCENT PROTEIN; NEOMYCIN; RED FLUORESCENT PROTEIN; RETROVIRUS VECTOR;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CANCER CELL CULTURE; CANCER GROWTH; CELL CLONE; CELL PROLIFERATION; CELL SELECTION; CELL TYPE; COLOR; CONTROLLED STUDY; EXPERIMENT; FEMALE; FIBROSARCOMA; FLUORESCENCE; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IMAGING; IN VIVO STUDY; INJECTION; LUNG METASTASIS; MOUSE; NONHUMAN; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; SKIN FLAP; SURFACE PROPERTY; SURGICAL TECHNIQUE; TAIL; THORAX WALL; TIME; VEIN;

ANIMALS; CELL DIVISION; CLONE CELLS; FEMALE; FIBROSARCOMA; GREEN FLUORESCENT PROTEINS; HUMANS; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; LUNG NEOPLASMS; MICE; MICE, SCID; MICROSCOPY, FLUORESCENCE; NEOPLASM TRANSPLANTATION; RETROVIRIDAE; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0344010549     PISSN: 02620898     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1027311230474     Document Type: Article

References (10)

1. Hoffman RM. Green fluorescent protein imaging of tumour growth, metastasis, and angiogenesis in mouse models. Lancet Oncol 2002; 3: 546-56.
2. Matz MV, Fradkov AF, Labas YA, et al. Fluorescent proteins from nonbioluminescent Anthozoa species. Nat Biotechnol 1999; 17: 969-73.
3. Heim R, Cubitt AB, Tsien RY. Improved green fluorescence. Nature 1995; 373: 663-4.
4. Chishima T, Miyagi Y, Wang X et al. Cancer invasion and micrometastasis visualized in live tissue by green fluorescent protein expression. Cancer Res 1997; 57: 2042-7.
5. Hasegawa S, Yang M, Chishima T et al. In vivo tumor delivery of the green fluorescent protein gene to report future occurrence of metastasis. Cancer Gene Ther 2000; 7: 1336-40.
6. Yang M, Baranov E, Jiang P et al. Whole-body optical imaging of green fluorescent protein-expressing tumors and metastases. Proc Natl Acad Sci USA 2000; 97: 1206-11.
7. Zolotukhin S, Potter M, Hauswirth WW et al. A 'humanized' green fluorescent protein cDNA adapted for high-level expression in mammalian cells. J Virol 1996; 70; 4646-54.
8. Yang M, Baranov E, Wang JW et al. Direct external imaging of nascent cancer, tumor progression, angiogenesis, and metastasis on internal organs in the fluorescent orthotopic model. Proc Natl Acad Sci USA 2002; 99; 3824-9.
9. Weissleder R, Ntziachristos V. Shedding light onto live molecular targets. Nat Med 2003; 9: 123-8.
10. Burgos JS, Rosol M, Moats RA et al. Time course of bioluminescent signal in orthotopic and heterotopic brain tumors in nude mice. BioTechniques 2003; 34: 1184-8.