Specific enzyme detection following isoelectric focusing as a complimentary tool for the differentiation of related Gadoid fish species

Food Chemistry

Volumn 70, Issue 2, 2000, Pages 241-245

  Piñeiro, Carmen ^a   Barros Velázquez, Jorge ^a   Pérez Martín, Ricardo I ^a   Gallardo, José M ^a  

^a INSTITUTO DE INVESTIGACIONES MARINAS   (Spain)

Author keywords

Enzyme detection; Gadoid fish species identification; Isoelectric focusing

Indexed keywords

ADENYLATE KINASE; GLYCEROL 3 PHOSPHATE DEHYDROGENASE; LACTATE DEHYDROGENASE; MALATE DEHYDROGENASE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; FISH; FOOD ANALYSIS; ISOELECTRIC FOCUSING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEA FOOD; SPECIES DIFFERENCE; STAINING;

GADIFORMES; MERLUCCIUS;

EID: 0343953526     PISSN: 03088146     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0308-8146(00)00042-X     Document Type: Article

References (24)

1. An H., Wei C.I., Zhao J., Marshall M.R., Lee C.M. Electrophoretic identification of fish species used in surimi products. Journal of Food Science. 54:1989;253-257.
2. Armstrong S.G., Leach D.N., Wyllie S.G. The use of HPLC-protein profiles in fish species identification. Food Chemistry. 44:1992;147-155.
3. Barlett S.E., Davidson W.S. Identification of Thunnus tuna species by the polymerase chain reaction and direct sequence analysis of their mitochondrial cytochrome b genes. Can. J. Fish. Aquat. Dis. 48:1991;309-317.
4. Bischoff K.M., Shi L., Kennelly P.J. The detection of enzyme activity following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Analytical Biochemistry. 260:1998;1-17.
5. Gabriel O., Gersten D.M. Staining for enzymatic activity after gel electrophoresis. Analytical Biochemistry. 203:1992;1-21.
6. Grant W.S., Becker I.I., Leslie R.W. Evolutionary divergence between sympatric species of southern African hakes, Merluccius capensis and M. paradoxus. I. Electrophoretic analysis of proteins. Heredity. 61:1988;13-20.
7. Keenan C.P., Shaklee J.B. Electrophoretic identification of raw and cooked fish fillets and other marine products. Food Technol. Aust. 37:1985;117-128.
8. King N.L. Species identification of cooked meats by enzyme-staining of isoelectric focusing gels. Meat Science. 11:1984;59-72.
9. Mackie I.M. A review of some recent applications of electrophoresis and isoelectric focusing in the identification of fish and fish products. Connnell J.J. Advances in fish science and technology. 1980;444-451 Fishing News Books, Farnharn.
10. Mackie I.M., Jones B.W. The use of electrophoresis of the water-soluble (sarcoplasmic) proteins of fish muscle to differentiate the closely related species of hake (Merluccius sp). Comparative Biochemistry and Physiology. 59B:1978;95-98.
11. Manchenko G.P. Handbook of detection of enzymes on electrophoretic gels. 1994;CRC Press Inc, Boca Raton.
12. 2 during the washing procedure. Appl. Theor. Electrophor. 2:1992;201-206.
13. Osman M.A., Ashoor S.H., Marsh P.C. Liquid chromatographic identification of common fish species. J. Assoc. Off. Anal. Chem. 70:1987;618-625.
14. Piñeiro C., Sotelo C.G., Medina I., Gallardo J.M., Pérez-Martín R.I. Reversed-phase HPLC as a method for the identification of gadoid fish species. Z. Lebensm. Unters. Forsch A. 204:1997;411-416.
15. Piñeiro C., Barros-Velázquez J., Sotelo C.G., Pérez-Martín R.I., Gallardo J.M. Two-dimensional electrophoretic study of the water-soluble protein fraction in white muscle of gadoid fish species. J. Agr. Food Chem. 46:1998;3991-3997.
16. Piñeiro C., Barros-Velázquez J., Pérez-Martín R.I., Martínez I., Jakobsen T., Rehbein H., Kündiger R., Mendes R., Etienne M., Jerome M., Craig A., Mackie I.M., Jessen F. Development of a sodium dodecyl sulfate-polyacrylamide gel electrophoresis reference method for the analysis and identification of fish species in raw and heat-processed samples: A collaborative study. Electrophoresis. 20:1999;1425-1432.
17. Piñeiro C., Barros-Velázquez J., Sotelo C.G., Gallardo J.M. The use of two-dimensional electrophoresis in the characterization of the water-soluble protein fraction of commercial flat fish species. Z. Lebensm. Unters. Forsch. A. 208:1999;342-348.
18. Quinteiro, J., Vidal, R., Rey-Méndez, M. (in press). Molecular analysis and biogeographic aspects of hake (Merluccius Rafinesque, 1810) based in sequences of the mitochondrial DNA control region. Mar. Biol.
19. Ramshaw J.A.M., Coyne J.A., Lewontin R.C. The sensitivity of gel electrophoresis as a detector of genetic variation. Genetics. 93:1979;1019-1037.
20. Redfield, J. A., & Salini, J. P. (1980). Techniques of starch-gel electrophoresis of penaeid prawn enzymes (Penaeus spp. and Metapenaeus spp.). CSIRO Aust. Div. Fish. Oceanogr. Rep. 116.
21. Rehbein H. Electrophoresis techniques for species identification. Z. Lebensm. Unters. Forsch. 191:1990;1-10.
22. Scobbie A.E., Mackie I.M. The use of sodium dodecyl sulphate polyacrylamide gel electrophoresis is fish species identification - a procedure suitable for cooked and raw fish. Journal of the Science of Food Agriculture. 44:1988;343-351.
23. Shaklee J.B., Allendorf F.W., Morizot D.C., Whitt G.S. Gene nomenclature for protein-coding loci in fish. Trans. Am. Fish. Soc. 119:1990;2-15.
24. Shaklee J.B., Keenan C.P. A practical laboratory guide to the techniques and methodology of electrophoresis and its application to fish fillet identification. 1986;CSIRO, Melbourne, Australia.