Role of hemagglutinin surface density in the initial stages of influenza virus fusion: Lack of evidence for cooperativity

Journal of Virology

Volumn 74, Issue 6, 2000, Pages 2714-2720

  Günther Ausborn, Susanne ^a,b   Schoen, Pieter ^b,d   Bartoldus, Ingrid ^a   Wilschut, Jan ^b,c   Stegmann, Toon ^c  

^a UNIVERSITY OF BASEL   (Switzerland)

^b INSTITUT DE PHARMACOLOGIE ET DE BIOLOGIE STRUCTURALE   (France)

^c UNIVERSITY OF GRONINGEN   (Netherlands)

^d HaemoProbe BV   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

LIPID; VIRUS HEMAGGLUTININ;

ARTICLE; BINDING AFFINITY; CONFORMATIONAL TRANSITION; ERYTHROCYTE; FLUORESCENCE; HUMAN; HUMAN CELL; INFLUENZA VIRUS; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; VIRUS REPLICATION;

ENDOCYTOSIS; ENDOSOMES; HEMAGGLUTININ GLYCOPROTEINS, INFLUENZA VIRUS; HUMANS; HYDROGEN-ION CONCENTRATION; INFLUENZA A VIRUS; MEMBRANE FUSION; TIME FACTORS;

EID: 0343621483     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.74.6.2714-2720.2000     Document Type: Article

References (40)

1. Bentz, J. 1992. Intermiediates and kinetics of membrane fusion. Biophys. J. 63:448-459.
2. Bentz, J., H. Ellens, and D. Alford. 1990. An architecture for the fusion site of influenza hemagglutinin. FEBS Lett. 276:1-5.
3. Blumenthal, R., D. Sarkar, S. Durell, D. E. Howard, and S. E. Morris. 1996. Dilation of the influenza hemagglutinin fusion pore revealed by the kinetics of individual cell-cell fusion events. J. Cell Biol. 135:63-71.
4. Blumenthal, R., C. Schoch, A. Puri, and M. J. Clague. 1991. A dissection of steps leading to viral envelope-mediated membrane fusion. Ann. N. Y. Acad. Sci. 635:285-296.
5. Böttcher, C. J. F., C. M. Van Gent, and C. Fries. 1961. A rapid and sensitive sub-micro phosphorous determination. Anal. Chim. Acta 24:203-204.
6. Bron, R., A. Ortiz, J. Dijkstra, T. Stegmann, and J. Wilschut. 1993. Preparation, properties and applications of reconstituted influenza virus envelopes. Methods Enzymol. 220:313-331.
7. Bron, R., A. Ortiz, and J. Wilschut. 1994. Cellular cytoplasmic delivery of a polypeptide toxin by reconstituted influenza virus envelopes (virosomes). Biochemistry 33:9110-9117.
8. Bullough, P. A., F. M. Hughson, J. J. Skehel, and D. C. Wiley. 1994. Structure of influenza haemagglutinin at the pH of membrane fusion. Nature 371:37-43.
9. Chernomordik, L. V., E. Leikina, V. Frolov, P. Bronk, and J. Zimmerberg. 1998. The pathway of membrane fusion catalyzed by influenza hemagglutinin: restriction of lipids, hemifusion, and lipidic fusion pore formation. J. Cell Biol. 140:1369-1382.
10. Chernomordik, L. V., and J. Zimmerberg. 1995. Bending membranes to the task: structural intermediates in bilayer fusion. Curr. Opin. Struct. Biol. 5:541-547.
11. Clague, M. J., C. Schoch, and R. Blumenthal. 1991. Delay time for influenza hemagglutinin-induced membrane fusion depends on the hemagglutinin surface density. J. Virol. 65:2402-2407.
12. Danieli, T., S. L. Pelletier, Y. I. Henis, and J. M. White. 1996. Membrane fusion mediated by the influenza hemagglutinin requires the concerted action of at least three hemagglutinin trimers. J. Cell Biol. 133:559-569.
13. Doms, R. W., and A. Helenius. 1986. Quaternary structure of influenza virus hemagglutinin after acid treatment. J. Virol. 60:833-839.
14. Ellens, H., J. Bentz, D. Mason, F. Zhang, and J. White. 1990. Fusion of influenza hemagglutinin-expressing fibroblasts with glycophorin-bearing liposomes: role of hemagglutinin surface density. Biochemistry 29:9697-9707.
15. Ellens, H., S. Doxsey, J. S. Glenn, and J. M. White. 1989. Delivery of macromolecules into cells expressing a viral membrane fusion protein. Methods Cell Biol. 31:155-178.
16. Folch, J., M. Lees, and G. H. Sloane-Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509.
17. Günther-Ausborn, S., A. Praetor, and T. Stegmann. 1995. Inhibition of influenza-induced membrane fusion by lysophosphatidylcholine. J. Biol. Chem. 270:29279-29285.
18. Keller, P., and K. Simons. 1998. Cholesterol is required for surface transport of influenza virus hemagglutinin. J. Cell Biol. 140:1357-1367.
19. Kemble, G. W., T. Danieli, and J. M. White. 1994. Lipid-anchored influenza hemagglutinin promotes hemifusion, not complete fusion. Cell 76:383-391.
20. Lamb, R. A., and R. M. Krug. 1996. Orthomyxoviridae, p. 1091-1152. In B. N. Fields, D. M. Knipe, and P. M. Howley (ed.), Fundamental virology. Lippincott-Raven, Philadelphia, Pa.
21. Ludwig, K., T. Korte, and A. Herrman. 1995. Analysis of delay times of hemagglutinin-mediated fusion between influenza virus and cell membranes. Eur. Biophys. J. 24:55-64.
22. Morris, S. J., D. P. Sarkar, J. M. White, and R. Blumenthal. 1989. Kinetics of pH-dependent fusion between 3T3 fibroblasts expressing influenza hemagglutinin and red blood cells. Measurement by dequenching of fluorescence. J. Biol. Chem. 264:3972-3978.
23. Peterson, G. L. 1977. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal. Biochem. 83:346-356.
24. Ramalho-Santos, J., S. Nir, N. Düzgünes, A. Pato de Carvalho, and M. da Conceiçāo Pedroso de Lima. 1993. A common mechanism for influenza virus fusion activity and inactivation. Biochemistry 32:2771-2779.
25. Sarkar, D. P., S. J. Morris, O. Eidelman, J. Zimmerberg, and R. Blumenthal. 1989. Initial stages of influenza hemagglutinin-induced cell fusion monitored simultaneously by two fluorescent events: cytoplasmic continuity and lipid mixing. J. Cell Biol. 109:113-122.
26. Scheiffele, P., A. Rietveld, T. Wilk, and K. Simons. 1999. Influenza viruses select ordered lipid domains during budding from the plasma membrane. J. Biol. Chem. 274:2038-2044.
27. Siegel, D. P. 1993. Modeling protein-induced fusion: insights from the relative stability of lipidic structures, p. 475-512. In J. Bentz (ed.), Viral fusion mechanisms. CRC Press, Boca Raton, Fla.
28. Simons, K., and E. Ikonen. 1997. Functional rafts in cell membranes. Nature 387:569-572.
29. Steck, T. L., and J. A. Kant. 1974. Preparation of impermeable ghosts and inside-out vesicles from human erythrocyte membranes. Methods Enzymol. 31:172-180.
30. Stegmann, T. 1993. Influenza hemagglutinin-mediated membrane fusion does not involve inverted phase lipid intermediates. J. Biol. Chem. 268:1716-1722.
31. Stegmann, T., I. Bartoldus, and J. Zumbrunn. 1995. Influenza hemagglutinin-mediated membrane fusion: influence of receptor binding on the lag phase preceding fusion. Biochemistry 34:1825-1832.
32. Stegmann, T., F. P. Booy, and J. Wilschut. 1987. Effects of low pH on influenza virus. Activation and inactivation of the membrane fusion capacity of the hemagglutinin. J. Biol. Chem. 262:17744-17749.
33. Stegmann, T., and A. Helenius. 1993. Influenza virus fusion: from models toward a mechanism, p. 89-111. In J. Bentz (ed.), Viral fusion mechanisms. CRC Press, Boca Raton, Fla.
34. Stegmann, T., D. Hoekstra, G. Scherphof, and J. Wilschut. 1986. Fusion activity of influenza virus: a comparison between biological and artificial target membrane vesicles. J. Biol. Chem. 261:10966-10969.
35. Stegmann, T., H. W. Morselt, F. P. Booy, J. F. van Breemen, G. Scherphof, and J. Wilschut. 1987. Functional reconstitution of influenza virus envelopes. EMBO J. 6:2651-2659.
36. Stegmann, T., J. M. White, and A. Helenius. 1990. Intermediates in influenza induced membrane fusion. EMBO J. 9:4231-4241.
37. Struck, D. K., D. Hoekstra, and R. E. Pagano. 1981. Use of resonance energy transfer to monitor membrane fusion. Biochemistry 20:4093-4099.
38. +-induced insertion of influenza hemagglutinin HA2 N-terminal segment into the viral membrane. J. Biol. Chem. 18535-18538.
39. Wilschut, J., and R. Bron. 1993. The influenza virus hemagglutinin: membrane fusion activity in intact virions and reconstituted virosomes, p. 133-161. In J. Bentz (ed.), Viral fusion mechanisms. CRC Press, Boca Raton, Fla.
40. Wilson, I. A., J. J. Skehel, and D. C. Wiley. 1981. Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 Å resolution. Nature 289:366-375.