Effects of triiodothyronine on the morphology of cells and matrix, the localization of alkaline phosphatase, and the frequency of apoptosis in long-term cultures of MC3T3-E1 cells

Bone

Volumn 20, Issue 3, 1997, Pages 225-236

  Fratzl Zelman, N ^a   Horandner H ^a   Luegmayr, E ^a   Varga, F ^a   Ellinger, A ^a   Erlee, M P M ^a   Klaushofer, Klaus ^a  

^a HANUSCH HOSPITAL   (Austria)

Author keywords

Alkaline phosphatase; Apoptosis; Histochemistry; Matrix morphology; MC3T3 E1 cells; Triiodothyronine

Indexed keywords

ALKALINE PHOSPHATASE; COLLAGEN FIBER; LIOTHYRONINE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL CULTURE; CELL NUCLEUS; CELL PROLIFERATION; CELL SHAPE; CELL SIZE; CELL STRAIN 3T3; CELL STRUCTURE; CONTROLLED STUDY; ELECTRON MICROSCOPY; ENZYME ACTIVITY; ENZYME LOCALIZATION; EXTRACELLULAR MATRIX; HISTOCHEMISTRY; MICROSCOPY; MOUSE; NONHUMAN; OSTEOBLAST;

ALKALINE PHOSPHATASE; APOPTOSIS; CELL LINE; EXTRACELLULAR MATRIX; MICROSCOPY, ELECTRON; OSTEOBLASTS; TRIIODOTHYRONINE;

EID: 0343581276     PISSN: 87563282     EISSN: None     Source Type: Journal    
DOI: 10.1016/S8756-3282(96)00367-5     Document Type: Article

References (55)

1. Amarnani, S., Merriman, H. L., Linkhart, T. A., Baylink, D., and Mohan, S. Autocrine regulators of MC3T3-E1 cell proliferation. J Bone Miner Res 8:157-165; 1993.
2. Aubin, J. A., Carlton, G., Bellows, C. G., Turksen, K., Liu, F., and Heersche, J. N. M. Analysis of the osteoblast lineage and regulation of differentiation. In: Slavkin, H. and Price, P., Eds. Chemistry and biology of mineralized tissues. The Netherlands: Elsevier Science; 267-276, 1992.
3. Aubin, J. E. and Turksen, K. Monoclonal antibodies as a tool for studying the osteoblast lineage. Microsc Res Technol 33:128-140; 1996.
4. Bonucci, E., Silvestrini, G., and Bianco, P. Extracellular alkaline phosphatase activity in mineralizing matrices of cartilage and bone: ultrastructural localization using a cerium-based method. Histochemistry 97:323-327; 1992.
5. Bos, M. P., van der Meer, J. M., Feyen, J. H. M., and Herrmann-Erlee, M. P. M. Expression of the parathyroid hormone receptor and correlation with other osteoblastic parameters in fetal rat osteoblasts. Calcif Tissue Int 58:95-100; 1996.
6. Chung, C. H., Golub, E. E., Forbes, E., Tokuoka, T., and Shapiro, I. M. Mechanism of action of β-glycerophosphate on bone cell mineralization. Calcif Tissue Int 51:305-311; 1992.
7. Claassen, H., Kampen, W. U., and Kirsch, T. Localization of collagens and alkaline phosphatase activity during mineralization and ossification of human first rib cartilage. Histochem Cell Biol 105:213-219; 1996.
8. Dean, D. D., Schwartz, Z., Bonewald, L., Muniz, O. E., Morales, S., Gomez, R., Brooks, B. P., Qiao, M., Howell, D. S., and Boyan, B. D. Matrix vesicles produced by osteoblast-like cells in culture become significantly enriched in proteoglycan-degrading metalloproteinases after addition of β-glycerophosphate and ascorbic acid. Calcif Tissue Int 54:399-408; 1994.
9. Evans-Storm, R. and Cidlowski, J. A. Regulation of apoptosis by steroid hormones. J Steroid Biochem Mol Biol 53:1-8; 1995.
10. Farley, J. R., Hall, S. L., Tanner, M. A., and Wergedal, J. E. Specific activity of skeletal alkaline phosphatase in human osteoblast-line cells regulated by phosphate, phosphate esters and phosphate analogs and release of alkaline phosphatase activity inversely regulated by calcium. J Bone Miner Res 9:497-508; 1994.
11. Fedarko, N. S., Bianco, P., Vetter, U., and Robey, P. G. Human bone cell enzyme activity and cellular heterogeneity: Correlation of alkaline phosphatase activity with cell cycle. J Cell Physiol 144:115-121; 1990.
12. Franceschi, R. T. and Iyer, B. S. Relationship between collagen synthesis and expression of the osteoblast phenotype in MC3T3-E1 cells. J Bone Miner Res 7:235-246; 1992.
13. Franceschi, R. T., Iyer, B. S., and Cui, Y. Effects of ascorbic acid on collagen matrix formation and osteoblast differentiation in murine MC3T3-E1 cells. J Bone Miner Res 9:843-854; 1994.
14. Gandrillon, O., Ferrand, N., Michailk, J. J., Roze, I., Zile, M. H., and Samarut, J. c-erbAα/T3R and RARs control commitment of hematopoetic self-renewing progenitor cells to apoptosis or differentiation and are antagonized by the v-erb,A. oncogene. Oncogene 9:749-758; 1994.
15. Gerstenfeld, L. C., Riva, A., Hodgens, K., Eyre, D. R., and Landis, W. J. Posttranslational control of collagen fibrillogenesis in mineralizing cultures of chick osteoblasts. J Bone Miner Res 8:1031-1043; 1993.
16. Glantschnig, H., Varga, F., and Klaushofer, K. Thyroid hormone and retinoic acid induce the synthesis of insulin-like growth factor-binding protein-4 in mouse osteoblastic cells. Endocrinology 137:281-286; 1996.
17. Gomez, S. and Boyde, A. Correlated alkaline phosphatase histochemistry and quantitative backscattered electron imaging in the study of rat incisor ameloblasts and enamel mineralization. Microsc Res Technol 29:29-36; 1994.
18. Gronowicz, G., Woodiel, F. N., McCarthy, M. B., and Raisz, L. G. In vitro mineralization of fetal rat parietal bones in defined serum-free medium: Effect of β-glycerophosphate. J Bone Miner Res 4:313-324; 1989.
19. 3) and dexamethasone interact to modulate osteoprogenitor cell differentiation in fetal rat calvaria cell culture. Bone 16:545-549; 1995.
20. Kasono, K., Sato, K., Han, D. C., Fujii, H., Tsushima, T., and Shizume, K. Stimulation of alkaline phosphatase activity by thyroid hormone in mouse osteoblast-like cells (MC3T3-E1): A possible mechanism of hyperalkaline phosphatasia in hyperthyroidism. Bone Miner 4:355-363; 1988.
21. Kassem, M., Mosekilde, L., and Eriksen, E. F. Effects of triiodothyronine on DNA synthesis and differentiation markers of normal human osteoblast-like cells in vitro. Biochem Mol Biol Int 30;779-788; 1993.
22. Klaushofer, K., Varga, F., Glantschnig, H., Fratzl-Zelman, N., Czerwenka, E., Leis, H. J., Koller, K., and Peterlik, M. The regulatory role of thyroid hormones in bone cell growth and differentiation. J Nutr 125:1996S-2003S; 1995.
23. Kodama, H., Amagai, Y., Sudo, H., Kasai, S., and Yamamoto, S. Establishment of a clonal osteogenic cell clone from newborn mouse calvaria. Jpn J Oral Biol 23:899-901; 1981.
24. Krieger, N. S., Stappenbeck, T. S., and Stern, P. H. Characterization of specific thyroid hormones receptors in bone. J Bone Miner Res 3:473-478; 1988.
25. Lissoos, T. W., Beno, D. W., and Davis, B. H. Postranslational inhibition of Ito cell type I collagen production by triiodothyronine. Am J Physiol 264:1090-1095; 1995.
26. Liu, F., Malaval, L., Gupta, A. K., and Aubin, J. E. Simultaneous detection of multiple bone-related mRNAs and protein expression during osteoblast differentiation: Polymerase chain reaction and immunocytochemical studies at the single cell level. Dev Biol 166:229-234; 1994.
27. Luegmayr, E., Varga, F., Frank, T., Roschger, P., and Klaushofer, K. Effects of triiodothyronine on morphology, growth behaviour and the actin cytoskeleton in mouse osteoblastic cells (MC3T3-E1). Bone 18:591-599; 1996.
28. Lynch, M. P., Stein, J. L., and Lian, J. B. The influence of type I collagen on the development and maintenance of the osteoblast phenotype in primary and passaged rat calvarial osteoblasts: Modification of expression of genes supporting cell growth, adhesion, and extracellular matrix maturation. Exp Cell Res 216:35-45; 1995.
29. Lynch, M. P., Stein, J. S., Stein, G. S., and Lian, J. B. Apoptosis during in vitro bone formation. J Bone Miner Res 9:S352; 1994 (abstr.).
30. Marsh, M. E., Munne, A. M., Vogel, J. J., Yingqi, C., and Franceshi, R. T. Mineralization of bone-like extracellular matrix in the absence of functional osteoblasts. J Bone Miner Res 10:1635-1634; 1995.
31. 3 of in vitro mineralization induced by osteoblast-like MC3T3-E1 cells. Bone 12:27-32; 1991.
32. McCauley, L. K., Koh, A. J., Beecher, C. A., Cui, Y., Decker, J. D., and Franceschi, R. T. Effects of differentiation and transforming growth factor β1 on PTH/PTHrP receptor mRNA levels in MC3T3-E1 cells. J Bone Miner Res 10:1243-1255; 1995.
33. McKee, M. D. and Nanci, A. Postembedding colloidal-gold immunocytochem-istry of non-collagenous extracellular matrix proteins in mineralized tissues. Microsc Res Technol 31:44-62; 1995.
34. Nanci, A., Zalzal, S., Gotoh, Y., and McKee, M. D. Ultrastructural localization and immunolocalization of osteopontin in rat calvarial osteoblast primary cultures. Microsc Res Technol 33:214-231; 1996.
35. Nishikawa, A. and Hayashi, H. Spatial, temporal and hormonal regulation of programmed muscle cell death during metamorphosis of the frog Xenopus laevis. Differentiation 59:207-214; 1995.
36. Ohishi, K., Ishida, H., Nagata, T., Yamauchi, N., Tsurumi, C., Nishikawa, S., and Wakano, Y. Thyroid hormone suppresses the differentiation of osteoprogenitor cells to osteoblasts, but enhances functional activities of mature osteoblasts in cultured rat calvaria cells. J Cell Physiol 161:544-552; 1994.
37. Oishi, K., Ishida, H., Nishikawa, S., Hamasaki, A., and Wakano, Y. The effect of triiodothyronine on cell growth and alkaline phosphatase activity of isolated rat calvarial cells. J Bone Miner Res 5:S299; 1990 (abstr).
38. Pockwinse, S. M., Stein, J. L., Lian, J. B., and Stein, G. S. Developmental stage specific cellular response to vitamin D and glucocorticoids during differentiation of the osteoblast phenotype: Interrelationship of morphology and gene expression by in situ hybridization. Exp Cell Res 216:244-260; 1995.
39. Pockwinse, S. M., Wilming, L. G., Conlon, D. M., Stein, G. S., and Lian, J. B. Expression of cell growth and bone specific genes at single cell resolution during development of bone tissue-like organization in primary osteoblast cultures. J Cell Biochem 49:310-323; 1992.
40. Quarles, D., Yohay, D. A., Lever, L. V., Caton, R., and Westrup, R. J. Distinct proliferative and differentiated stages of murine MC3T3-E1 cells in culture: An in vitro model of osteoblast development. J Bone Miner Res 7:683-692; 1992.
41. Robinson, J. M. Improved localization of intracellular sites of phosphatases using cerium and cell permeabilization. J Histochem Cytochem 33:749-754; 1985.
42. Sato, K., Han, D. C., Fujii, Y., Tshushima, T., and Shizume, K. Thyroid hormone stimulates alkaline phosphatase activity in cultured rat osteoblastic cells (ROS 17/2.8) through 3,5,3′-triiodo-L-thyronine nuclear receptor. Endocrinology 120:1873-1881; 1987.
43. Stein, G. S. and Lian, J. S. Molecular mechanisms mediating proliferation/differentiation interrelationships during progressive development of the osteoblast phenotype. Endocr Rev 14:424-442; 1993.
44. Sudo, H., Kodama, H., Amagai, Y., Yamamoto, S., and Kasai, S. In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol 96:191-198; 1983.
45. Varga, F., Rumper, M., Fratzl-Zelman, N., Glantschnig, H., and Klaushofer, K. Triiodothyronine stimulates osteocalcin expression in MC3T3-E1 cells. J Bone Miner Res 11:5264; 1996 (abstr.).
46. 3) treatment of MC3T3-E1 cells prevents specific response to EGF. Bone Miner 25(Suppl 1):S26; 1994 (abstr.).
47. Varga, F., Rumpler, M., and Klaushofer, K. Thyroid hormones increase insulin-like growth factor mRNA levels in the clonal osteoblastic cell line MC3T3-E1. FEBS Lett 345:67-70; 1994.
48. Watson, P. L., Kang, Y. H., and Falk, M. C. Cytochemical properties of osteoblast cell membrane domains. J Histochem Cytochem 37:1235-1246; 1989.
49. Welsh, J. O. Induction of apoptosis in breast cancer cells in response to vitamin D and antiestrogens. Biochem Cell Biol 72:537-545; 1994.
50. White, E. Life, death, and the pursuit of apoptosis. Gen Dev 10:1-15; 1996.
51. Williams, G. R., Bland, R., and Sheppard, M. C. Characterization of thyroid hormone receptors in three osteosarcoma cell lines of distinct phenotype: Interactions among T3, vitamin D3, and retinoid signalling. Endocrinology 135: 2375-2385; 1994.
52. Williams, G. R., Bland, R., and Sheppard, M. C. Retinoids modify regulation of endogenous gene expression by vitamin D3 and thyroid hormone in three osteosarcoma cell lines. Endocrinology 136:4304-4314; 1995.
53. Yang, R. S., Liu, T. K., Tsai, K. S., Lin-Shiau, S. Y., and Lu, K. S. Morphological and immunocytochemical characterization of osteoblast cultures from long bones of neonatal rats. Arch Histol Cytol 55:415-422; 1992.
54. Yao, J. and Eghbali, M. Decreased collagen mRNA and regression of cardiac fibrosis in the ventricular myocardium of the tight skin mouse following thyroid hormone treatment. Cardiovasc Res 26:603-607; 1992.
55. Yohay, D. A., Zhang, J., Thraikill, K. M., Arthur, J. M., and Quarles, L. D. Role of serum in the developmental expression of alkaline phosphatase in MC3T3-E1 osteoblasts. J Cell Physiol 158:467-475; 1994.