Phyllanthus orbicularis aqueous extract: Cytotoxic, genotoxic, and antimutagenic effects in the CHO cell line

Toxicology and Applied Pharmacology

Volumn 161, Issue 3, 1999, Pages 231-239

  Sànchez Lamar, Angel ^a   Fiore, Mario ^b   Cundari, Enrico ^b   Ricordy, Ruggero ^b   Cozzi, Renata ^c   De Salvia, Rosella ^b  

^a UNIVERSITY OF HAVANA   (Cuba)

^b CNR   (Italy)

^c ROMA TRE UNIVERSITY   (Italy)

Author keywords

Antimutagenicity; Apoptosis; CHO; Cytotoxicity; Genotoxicity; Phyllanthus

Indexed keywords

DNA; PLANT EXTRACT;

ANIMAL CELL; ANTIVIRAL ACTIVITY; APOPTOSIS; ARTICLE; CELL CYCLE; CELL GROWTH; CELL SURVIVAL; CHO CELL; CHROMOSOME ABERRATION; COLONY FORMING UNIT; CONTROLLED STUDY; CYTOTOXICITY; DRUG ACTIVITY; GENOTOXICITY; GROWTH INHIBITION; HEPATITIS A VIRUS; HEPATITIS B VIRUS; INFLUENZA VIRUS B; KINETICS; MITOSIS INDEX; NONHUMAN; PHYLLANTHUS;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; HEPATITIS A VIRUS; HEPATITIS B VIRUS; INFLUENZA VIRUS; INFLUENZAVIRUS B; PHYLLANTHUS ORBICULARIS;

EID: 0343550418     PISSN: 0041008X     EISSN: None     Source Type: Journal    
DOI: 10.1006/taap.1999.8814     Document Type: Article

References (30)

1. Bass D A., Parce J. W., Dechatelet L. R., Szejda P., Seeds M. C., Thomas M. Flow cytometric studies of oxidative product formation by neutrophils: A graded response to membrane stimulation. J. Immunol. 130:1983;1910-1917.
2. Bijur G. N., Ariza M. E., Hitchcock Ch. L., Williams M. V. Antimutagenic and promutagenic activity of ascorbic acid during oxidative stress. Environ. Mol. Mutagen. 30:1997;339-345.
3. Cozzi R., Ricordy R., Aglitti T., Gatta V., Perticone P., De Salvia R. Ascorbic acid and β-carotene as modulators of oxidative damage. Carcinogenesis. 18:1997;223-228.
4. Del Barrio G., Caballero O., Chevalier P. Inactivation in vitro del Ags HB por extractos de plantas del genero Phyllanthus. Rev.Cubana Med. Trop. 47:1995;127-130.
5. Dhir H., Kumar A., Sharma A. Relative efficiency of Phyllanthus emblica fruit extract and ascorbic acid in modifying lead and aluminium-induced sister chromatid exchanges in mouse bone marrow. Environ. Mol. Mutag. 21:1993;229-236.
6. Dhir H. A., Kumar A., Sharma P., Talukder G. Modification of clastogenicity of lead and aluminium in mouse bone marrow cells by dietary ingestion of Phyllanthus emblica fruit extract. Mutat. Res. 241:1990;305-312.
7. Filho C.V., Santos A.R., De Campos R. O., Miguel O. G., Yunes R. A., Ferrari F. I., Messana G., Calixto J. B. Chemical and pharmacological studies of Phyllanthus carolinensis in mice. J. Pharm. Pharmacol. 48:1996;1231-1236.
8. Fonseca, G., and Ferrer, M.1995. Detecciòn in vivo del efecto genotòxico de un extracto antiviral de Phyllanthus orbicularis Rev. Cubana de Farmacia., Suppl. Especial, Vol. 30, VI Congreso Int. de Farmacia, Ciudad de la Habana Cuba, p. 214.
9. Gowrishanker B., Vivekanandan O. S. In vivo studies of a crude extract of Phyllanthus Amarus L. in modifying the genotoxicity induced in Vicia faba L. by tannery effluents. Mutat. Res. 322:1994;185-192.
10. Gupta M. L., Gupta T. K., Bhargava F. P. A study of antifertility effects of some indigenous drugs. J. Res. Indian Med. 6:1971;112-117.
11. Ihantola-Vormisto A. J., Summanen H., Kankaaranta H., Vuorela Z.M., Asmawi C., Moilanen E. Anti-inflammatory activity of extracts from leaves of Phyllanthus emblica. Planta Med. 63:1997;518-524.
12. Ianas O., Olinescu R., Badescu I. Melatonin involvement in oxidative processes. Endocrinology. 29:1991;147-153.
13. Imanishi H., Sasaki Y. F., Ohta T., Watanabe M., Kato T., Shirasu Y. Tea tannin components modify the induction of sister chromatid exchanges and chromosome aberrations in mutagen treated cultured mammalian cells and mice. Mutat. Res. 259:1991;79-87.
14. Jinsart W., Ternai B., Polya G. M. Inhibition of rat liver cyclic AMP-dependent protein kinase by flavonoids. Biol. Chem. Hoppe-Seyler. 373:1992;205-211.
15. Kirsch-Volders M., Elhajouji A., Cundari E., Van Hummelen P. The in vitro micronucleus test: A multi-endpoint assay to detect simultaneously mitotic delay, apoptosis, chromosome breakage, chromosome loss and non-disjunction. Mutat. Res. 392:1997;19-30.
16. Kuroda Y., Hara Y. Antimutagenic and anticarcinogenic activity of tea polyphenols. Mutat.Res. 436:1999;69-97.
17. Marshall K., Reiter R. J., Poeggeler B., Aruoma O. I., Halliwell B. Evaluation of the antioxidant activity of melatonin in vitro. Free Radical Biol. Med. 21:1996;307-315.
18. Middleton E. Jr. Effect of plant flavonoids on immune and inflammatory cell function. Adv. Exp. Med. Biol. 439:1998;175-182.
19. Omulokoli E., Khan B., Chabra S. C. Antiplasmodial activity of four Kenyan medicinal plants. J. Ethnopharmacol. 56:1997;133-137.
20. Ott M., Thyagarajan S.P., Gupta S. suppresses hepatitis B virus by interrupting interactions between HBV enhancer I and cellular transcription factors, Eur. J. Clin. Invest. 27:1997;908-915.
21. Palitti F., Fiore M., De Salvia R., Tanzarella C., Ricordy R., Forster R., Mosesso P., Astolfi E., Loprieno N. Tests for the induction of chromosomal aberrations in Chinese hamster ovary (CHO) cells in culture. Prog. Mutat. Res. 5:1985;443-451.
22. Rivera, F, Vidal, A, Lòpez, T, Ferrer, E. M, Sotolongo, M, and, Medina, M. E. 1995. Toxicidad aguda y subcrònica de un extracto de, Phyllanthus orbicularis, en: Revista Resumen del IV Congreso de la Soc. Cubana de C. Farmaceùticas, Ciudad de la Habana, Cuba, p, 35.
23. Roig, J. T. 1988. Plantas medicinales, aromàticas o venenosas de Cuba, T I, ed. Cientìfico-Tècnica, La Habana, Cuba, p, 283.
24. Scholz M., Blaheta R. A., Markus B. H., Doerr H. W., Cinatl J. Immunomodulation and anticytomegalovirus activity of antioxidant metal chelators. Transplant. Proc. 29:1997;1272-1273.
25. Shamberger R. J. Genetic toxicology of ascorbic acid. Mutat.Res. 133:1984;135-159.
26. Tan D. X., Chen L. D., Poeggeler B., Manchester L. C., Reiter R. J. Melatonin: a potent, endogenous hydroxyl radical scavenger. Endocr. J. 1:1993;57-60.
27. Thyagarajan S. P., Thirunalasundari T., Subramanian S., Venkateswaran P. S., Blumberg B. S. Effect of Phyllanthus amarus on chronic carriers of hepatitis B virus. Lancet. 2:1988;764-766.
28. Tuosto L., Cundari E., Montani M. S., Piccolella E. Analysis of susceptibility of mature human lymphocytes to dexamethasone-induced apoptosis. Eur. J.Immunol. 24:1994;1061-1065.
29. Vinayagamoothy T. Antibacterial activity of medicinal plants of Sri Lanka, Ceylon. J. Sci. Biol. Soc. 191:1982;50-59.
30. Young I. T. Proof without prejudice: Use of Kolmogorov-Smirnov test for the analysis of histograms from flow systems and other sources. J. Histochem. Cytochem. 25:1977;935-939.