Peptides that block hepatitis B virus assembly: Analysis by cryomicroscopy, mutagenesis and transfection

EMBO Journal

Volumn 17, Issue 23, 1998, Pages 6839-6845

  Bottcher B ^a,d   Tsuji, N ^c   Takahashi, H ^c   Dyson, M R ^b,e   Zhao, S ^b   Crowther, R A ^a   Murray, K ^b  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

^c MASSACHUSETTS GENERAL HOSPITAL   (United States)

^d UNIVERSITY OF FREIBURG   (Germany)

^e Peptide Therapeutics Ltd   (United Kingdom)

Author keywords

Difference imaging; Electron cryomicroscopy; Hepatitis B virus; Peptide inhibitors; Virus assembly

Indexed keywords

ALANINE; HEPATITIS B SURFACE ANTIGEN; PEPTIDE;

ARTICLE; CRYOELECTRON MICROSCOPY; GENETIC TRANSFECTION; HEPATITIS B VIRUS; HEPATOMA CELL; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; VIRUS ASSEMBLY;

DNA VIRUSES; HEPATITIS B VIRUS;

EID: 0343147172     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.23.6839     Document Type: Article

References (28)

1. Bellare, J.R., Davis, H.T., Scriven, L.E. and Talmon, Y. (1988) Controlled environment vitrification system: an improved sample preparation technique. J. Electron Microsc. Tech., 10, 87-111.
2. Block, T.M. et al. (1998) Treatment of chronic hepadnavirus infection in a woodchuck animal model with an inhibitor of protein folding and trafficking. Nature Med., 4, 610-614.
3. Böttcher, B. and Crowther, R.A. (1996) Difference imaging reveals ordered regions of RNA in turnip yellow mosaic virus. Structure, 4, 387-394.
4. Böttcher, B., Wynne, S.A. and Crowther, R.A. (1997) Determination of the fold of the core protein of heptatitis B virus by electron cryomicroscopy. Nature, 386, 88-91.
5. Bruss, V. and Ganem, D. (1991) The role of envelope protein in hepatitis B virus assembly. Proc. Natl Acad. Sci. USA, 88, 1059-1063.
6. Bruss, V. Lu. X., Thomssen, R. and Gerlich, W.H. (1994) Post-translational alteration in transmembrane topology of hepatitis B virus large envelope protein. EMBO J., 13, 2273-2279.
7. Collier, N.C., Knox, K. and Schlesinger, M.J. (1991) Inhibition of influenza virus formation by a peptide that corresponds to sequences in the cytoplasmic domain of the hemagglutinin. Virology, 183, 769-772.
8. Collier, N.C., Adams, S.P., Weingarten, H. and Schlesinger, M.J. (1992) Inhibition of enveloped RNA virus formation by peptides corresponding to glycoprotein sequences. Antiviral Chem. Chemother., 3, 31-36.
9. Conway, J.F., Cheng, N., Zlotnick, A., Wingfield, P.T., Stahl, S.J. and Steven, A.C. (1997) Visualization of a 4-helix bundle in the hepatitis B virus capsid by cryo-electron microscopy. Nature, 386, 91-94.
10. Conway, J.E. Cheng, N., Zlotnick, A., Stahl, S.J., Wingfield, P.T., Belnap, D.M., Kanngiesser, U., Noah, M. and Steven, A.C. (1998) Hepatitis B virus capsid: localization of the putative immunodominant loop (residues 78 to 83) on the capsid surface and implications for the distinction between c and e-antigens. J. Mol. Biol., 279, 1111-1121.
11. Crowther, R.A., Kiselev, N.A., Böttcher, B., Berriman, J.A., Borisova, G.P., Ose, V and Pumpens, P. (1994) Three-dimensional structure of hepatitis B virus core particles determined by electron microscopy. Cell, 77, 943-950.
12. Dyson, M.R. and Murray, K. (1995) Selection of peptide inhibitors of interactions involved in complex protein assemblies: association of the core and surface antigens of hepatitis B virus. Proc. Natl Acad. Sci. USA, 92, 2194-2198.
13. Dyson, M.R., Germaschewski, V. and Murray, K. (1995) Direct measurement via phage titre of the dissociation constants in solution of fusion phage-substrate complexes. Nucleic Acids Res., 23, 1531-1535.
14. Kunkel, T.A. (1985) Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl Acad. Sci. USA, 82, 488-492.
15. Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: application of proliferation and cytotoxicity assays. J. Immunol. Methods, 65, 55-63.
16. Neurath, A.R., Kent, S.B.H., Strick, N. and Parker, K. (1986) Identification and chemical synthesis of a host cell receptor binding site on hepatitis B virus. Cell, 46, 429-136.
17. Pasek, M., Goto, T., Gilbert, W., Zink, B., Schaller, H., MacKay, P., Leadbetter, G. and Murray, K. (1979) Hepatitis B virus genes and their expression in E.coli. Nature, 282, 575-579.
18. Ryan, F. (1992) Tuberculosis: The Greatest Story Never Told, Swift Publishers, Bromsgrove, UK.
19. Salfeld, J., Pfaff, E., Noah, M. and Schaller, H. (1989) Antigenic determinants and functional domains in core antigen and e antigen from hepatitis B virus. J. Virol., 63, 798-808.
20. Sällberg, M., Ruden, U. and Magnius, L.O. (1991) Characterisation of a linear binding site for a monoclonal antibody to hepatitis B core antigen. J. Med. Virol., 33, 248-252.
21. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
22. Smith, G.P. and Scott, J.K. (1993) Libraries of peptides and proteins displayed on filamentous phage. Methods Enzymol., 217, 228-257.
23. Staros, J.V., Wright, R.W. and Swingle, D.M. (1986) Enhancement by N-hydroxysulfosuccinimide of water-soluble carbondiimide-mediated coupling reactions. Anal. Biochem., 156, 220-222.
24. Stewart, F.J. (1993) Mutations that affect the structure and interactions of the core antigen of hepatitis B virus. PhD thesis, University of Edinburgh, Scotland.
25. Takahashi, H., Kameda, H. and Wands, J.R. (1988) Development of a second generation monoclonal immunoradiometric assay. Increased sensitivity leads to enhanced detection of hepatitis B viral infection. J. Immunol. Methods, 112, 191-200.
26. Takahashi, H., Fujimoto, J., Hanada, S. and Isselbacher, K.J. (1995) Acute hepatitis in rats expressing human hepatitis B virus transgenes. Proc. Natl Acad. Sci. USA, 92, 1470-1474.
27. Teschke, C.M., King, J. and Prevelige, P.E. (1993) Inhibition of viral capsid assembly by 1,1′-bi (4-anilinonaphthalene)-5-sulfonic acid. Biochemistry, 32, 10658-10665.
28. Ueda, K., Tsurimoto, K. and Matsubara, K. (1991) Three envelope proteins of hepatitis B virus: large S, middle S and major S proteins needed for the formation of Dane particles. J. Virol., 65, 3521-3529.