Assembly and functional organization of the nucleolus: Ultrastructural analysis of Saccharomyces cerevisiae mutants

Molecular Biology of the Cell

Volumn 11, Issue 6, 2000, Pages 2175-2189

  Trumtel, Stéphanie ^a   Léger Silvestre, Isabelle ^a   Gleizes, Pierre Emmanuel ^a   Teulières, Frédéric ^a   Gas, Nicole ^a  

^a CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

DNA DIRECTED RNA POLYMERASE; PROTEIN; RIBOSOME DNA; RIBOSOME RNA; RNA POLYMERASE II;

ARTICLE; CHROMOSOME STRUCTURE; CONTROLLED STUDY; GENE; MUTANT; NONHUMAN; NUCLEOLUS; PLASMID; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SACCHAROMYCES CEREVISIAE; ULTRASTRUCTURE;

SACCHAROMYCES CEREVISIAE;

EID: 0343081680     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.11.6.2175     Document Type: Article

References (41)

1. Adams, B.G. (1972). Induction of galactokinase in Saccharomyces cerevisiae: kinetics of induction and glucose effects. J. Bacteriol. 111, 308-315.
2. Azum-Gelade, M.C., Noaillac-Depeyre, J., Caizergues-Ferrer, M., and Gas, N. (1994). Cell cycle redistribution of U3 snRNA and fibrillarin. Presence in the cytoplasmic nucleolus remnant and in the prenucleolar bodies at telophase. J. Cell Sci. 107, 463-475.
3. Benavente, R., Rose, K.M., Reimer, G., Hugle-Dorr, B., and Scheer, U. (1987). Inhibition of nucleolar reformation after microinjecrion of antibodies to RNA polymerase I into mitotic cells. J. Cell Biol. 105, 1483-1491.
4. Bonneaud, N., Ozier-Kalogeropoulos, O., Li, G.Y., Labouesse, M., Minvielle-Sebastia, L., and Lacroute, F. (1991). A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7, 609-615.
5. Bousquet-Antonelli, C., Henry, Y., Gelugne, J.P., Caizergues-Ferrer, M., and Kiss, T. (1997). A small nucleolar RNP protein is required for pseudouridylation of eukaryotic ribosomal RNAs. EMBO J. 16, 4770-4776.
6. Chernoff, Y.O., Vincent, A., and Liebman, S.W. (1994). Mutations in eukaryotic 18S ribosomal RNA affect translational fidelity and resistance to aminoglycoside antibiotics. EMBO J. 13, 906-913.
7. Clark, M.W., Yip, M.L., Campbell, J., and Abelson, J. (1990). SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs. J. Cell Biol. 111, 1741-1751.
8. Cormack, B.P., Valdivia, R.H., and Falkow, S. (1996). FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173, 33-38.
9. Elliott, D.J., Bowman, D.S., Abovich, N., Fay, F.S., and Rosbash, M. (1992). A yeast splicing factor is localized in discrete subnuclear domains. EMBO J. 11, 3731-3736.
10. Gietz, R., and Schiestl, R.H. (1995). Transforming yeast with DNA. Methods Mol. Cell. Biol. 5, 255-269.
11. Girard, J.P., Bagni, C., Caizergues-Ferrer, M., Amalric, F., and Lapeyre, B. (1994). Identification of a segment of the small nucleolar ribonucleoprotein-associated protein GAR1 that is sufficient for nucleolar accumulation. J. Biol. Chem. 269, 18499-18506.
12. Girard, J.P., Lehtonen, H., Caizergues-Ferrer, M., Amalric, F., Tollervey, D., and Lapeyre, B. (1992). GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast. EMBO J. 11, 673-682.
13. Goessens, G. (1984). Nucleolar structure. Int. Rev. Cytol. 87, 107-158.
14. Hadjiolov, A.A. (1985). The Nucleolus and Ribosome Biogenesis, Vienna: Springer-Verlag.
15. Hernandez-Verdun, D., Robert-Nicoud, M., Geraud, G., and Masson, C. (1991). Behavior of nucleolar proteins in nuclei lacking ribosomal genes. A study by confocal laser scanning microscopy. J. Cell Sci. 98, 99-105.
16. Hirano, T., Konoha, G., Toda, T., and Yanagida, M. (1989). Essential roles of the RNA polymerase I largest subunit and DNA topoisomerases in the formation of fission yeast nucleolus. J. Cell Biol. 108, 243-253.
17. Hughes, J.M., and Ares, M., Jr. (1991). Depletion of U3 small nucleolar RNA inhibits cleavage in the 5′ external transcribed spacer of yeast pre-ribosomal RNA and impairs formation of 18S ribosomal RNA. EMBO J. 10, 4231-4239.
18. Keys, D.A., Vu, L., Steffan, J.S., Dodd, J.A., Yamamoto, R.T., Nogi, Y., and Nomura, M. (1994). RRN6 and RRN7 encode subunits of a multiprotein complex essential for the initiation of rDNA transcription by RNA polymerase I in Saccharomyces cerevisiae. Genes Dev. 8, 2349-2362.
19. Kostura, M., and Craig, N. (1986). Treatment of Chinese hamster ovary cells with the transcriptional inhibitor actinomycin D inhibits binding of messenger RNA to ribosomes. Biochemistry 25, 6384-6391.
20. Lafontaine, D.L., and Tollervey, D. (1999). Nop58p is a common component of the box C+D snoRNPs that is required for snoRNA stability. RNA 5, 455-467.
21. Lazdins, I.B., Delannoy, M., and Sollner-Webb, B. (1997). Analysis of nucleolar transcription and processing domains and pre-rRNA movements by in situ hybridization. Chromosoma 105, 481-495.
22. Léger-Silvestre, I., Noaillac-Depeyre, J., Faubladier, M., and Gas, N. (1997). Structural and functional analysis of the nucleolus of the fission yeast Schizosaccharomyces pombe. Eur. J. Cell Biol. 72, 13-23.
23. Léger-Silvestre, I., Trumtel, S., Noaillac-Depeyre, J., and Gas, N. (1999). Functional compartimentalization of the nucleus in the budding yeast Saccharomyces cerevisiae. Chromosoma 108, 103-113.
24. Lo, H.J., Huang, H.K., and Donahue, T.F. (1998). RNA polymerase I-promoted HIS4 expression yields uncapped, polyadenylated mRNA that is unstable and inefficiently translated in Saccharomyces cerevisiae. Mol. Cell. Biol. 18, 665-675.
25. Nierras, C.R., Liebman, S.W., and Warner, J.R. (1997). Does Saccharomyces need an organized nucleolus? Chromosoma 105, 444-451.
26. Nogi, Y., Yano, R., and Nomura, M. (1991). Synthesis of large rRNAs by RNA polymerase II in mutants of Saccharomyces cerevisiae defective in RNA polymerase I. Proc. Natl. Acad. Sci. USA 88, 3962-3966.
27. Oakes, M., Aris, J.P., Brockenbrough, J.S., Wai, H., Vu, L., and Nomura, M. (1998). Mutational analysis of the structure and localization of the nucleolus in the yeast Saccharomyces cerevisiae. J. Cell Biol. 143, 23-34.
28. Oakes, M., Nogi, Y., Clark, M.W., and Nomura, M. (1993). Structural alterations of the nucleolus in mutants of Saccharomyces cerevisiae defective in RNA polymerase I. Mol. Cell. Biol. 13, 2441-2455.
29. Petes, T.D. (1979). Yeast ribosomal DNA genes are located on chromosome XII. Proc. Natl. Acad. Sci. USA 76, 410-414.
30. Pierron, G., and Puvion-Dutilleul, F. (1996). Localization of the newly initiated and processed ribosomal primary transcripts during the mitotic cycle in Physarum polycephalum. Exp. Cell Res. 229, 407-420.
31. Planta, R.J. (1997). Regulation of ribosome synthesis in yeast. Yeast 13, 1505-1518.
32. Puvion-Dutilleul, F., and Bachellerie, J.P. (1979). Ribosomal transcriptional complexes in subnuclear fractions of Chinese hamster ovary cells after short-term actinomycin D treatment. J. Ultrastruct. Res. 66, 190-199.
33. Scheer, U., and Raska, I. (1987). Immunocytochemical localization of RNA polymerase I in the fibrillar centers of nucleoli. Chromosomes Today 9, 284-294.
34. Shaw, P.J., and Jordan, E.G. (1995). The nucleolus. Annu. Rev. Cell Dev. Biol. 11, 93-121.
35. Sisodia, S.S., Sollner-Webb, B., and Cleveland, D.W. (1987). Specificity of RNA maturation pathways: RNAs transcribed by RNA polymerase III are not substrates for splicing or polyadenylation. Mol. Cell. Biol. 7, 3602-3612.
36. Steffan, J.S., Keys, D.A., Dodd, J.A., and Nomura, M. (1996). The role of TBP in rDNA transcription by RNA polymerase I in Saccharomyces cerevisiae: TBP is required for upstream activation factor-dependent recruitment of core factor. Genes Dev. 10, 2551-2563.
37. Tollervey, D., Lehtonen, H., Carmo-Fonseca, M., and Hurt, E.C. (1991). The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast. EMBO J. 10, 573-583.
38. Verheggen, C., Le Panse, S., Almouzni, G., and Hernandez-Verdun, D. (1998). Presence of pre-rRNAs before activation of polymerase I transcription in the building process of nucleoli during early development of Xenopus laevis. J. Cell Biol. 142, 1167-1180.
39. Wachtler, F., and Stahl, A. (1993). The nucleolus: a structural and functional interpretation. Micron 24, 473-505.
40. Williams, M.A. (1977). Quantitative Methods in Biology, Amsterdam: Elsevier/North Holland.
41. Wittekind, M., Dodd, J., Vu, L., Kolb, J.M., Buhler, J.M., Sentenac, A., and Nomura, M. (1988). Isolation and characterization of temperature-sensitive mutations in RPA190, the gene encoding the largest subunit of RNA polymerase I from Saccharomyces cerevisiae. Mol. Cell. Biol. 8, 3997-4008.