Production of xylanase by Aspergillus awamori on synthetic medium in stirred tank and airlift tower loop reactors: The influence of stirrer speed and phosphate concentration

Journal of Biotechnology

Volumn 56, Issue 2, 1997, Pages 103-114

  Siedenberg, D ^a   Gerlach, S R ^a   Weigel, B ^a   Schugerl, K ^b   Giuseppin, M L F ^b   Hunik, J ^b  

^a LEIBNIZ UNIVERSITY HANNOVER   (Germany)

^b UNILEVER RESEARCH VLAARDINGEN   (Netherlands)

Author keywords

Airlift tower loop reactor; Aspergillus awamori; Carbon balance; Pellets; Phosphate effect; Stirred tank; Stirrer speed effect; Xylanase

Indexed keywords

BIOREACTORS; CATALYST ACTIVITY; CELL CULTURE; COMPOSITION EFFECTS; ENZYMES; GLUCOSE; MORPHOLOGY; PHOSPHATES;

AIRLIFT TOWER LOOP REACTOR; ASPERGILLUS AWAMORI; STIRRED TANK REACTOR; XYLANASE;

FUNGI;

XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; ASPERGILLUS AWAMORI; CULTURE MEDIUM; ENGINEERING; ENZYME SYNTHESIS; FUNGUS GROWTH; NONHUMAN; PRIORITY JOURNAL; SPOROGENESIS;

EID: 0343036306     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(97)00081-3     Document Type: Article

References (23)

1. Adolph, S., Muller, S., Siedenberg, D., Jager, K., Lehmann, H., Schugerl, K., Giuseppin, M., 1996. Cultivation of Aspergillus awamori on wheat bran: Ultrastructural description of growth and localization of xylanase by immunogold labeling. J. Biotechnol. 46, 221-234.
2. Aguilar, G., Huiton, C., 1987. Stimulation of the productipon of extracellular pectinolytic activities of Aspergillus sp. by galacturonic acid and glucose addition. Enzyme Microb. Technol. 9, 690-696.
3. Burnett, T.J., Chavira, R., Hagemann, J.H., 1984. Assaying proteinases with Azocoll. Anal. Biochem. 136, 446-450.
4. Calam, C.T., 1987. Process Development in Antibiotic Fermentations, Cambridge University Press, Cambridge.
5. Deschamps, F., Huet, M.C., 1985. Xylanase production in solid-state fermentation: a study of its properties. Appl. Microbiol. Bioetchnol. 22, 177-180.
6. Freudenberg, S., Fasold, K.-I., Muller, S.R., Siedenberg, D., Kretzmer, G., Schugerl, K., Giuseppin, M., 1996. Fluorescence microscopic investigation of Aspergillus awamori growing on synthetic and complex media and producing xylanase. J. Biotechnol. 46, 265-273.
7. Gerlach, S.R. (1997) Morphologische Untersuchungen an Aspergillus awamori Dissertation. University of Hanover, Hanover.
8. Ghosh, M., Das, A., Mishra, A.K., Nanda, G., 1993. Aspergillus sydowii MG49 is a strong producer of thermostable xylanolytic enzymes. Enzyme Microb. Technol. 15, 703-709.
9. Hoq, M.M., Hempel, C., Deckwer, W.D., 1994. Cellulase-free xylanase by Thermomyces lanuginosus RT9: effect of agitation, aeration, and medium components on production. J. Biotechnol. 37, 49-58.
10. Kleber, H.P., Schlee, D., Schopp, W., 1988. Biochemisches Praktikum, 3rd ed. Gustav Fischer Verlag, Berlin.
11. McCrae, S.I., Leith, K.M., Gordon, A.H., Wood, T.M., 1994. Xylan-degrading enzyme system produced by the fungus Aspergillus awamori: isolation and characterization of a feruloyl esterase and a p-coumaroyl esterase. Enzyme Microb. Technol. 16, 826-834.
12. Miller, G.L., 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Chem. 31, 426-428.
13. Nowakowska-Waszczuk, A., Sokolowski, A., 1987. Application of carbon balance to submerged citric acid production by Aspergillus niger.. Appl. Microbiol. Biotechnol. 26, 363-364.
14. Pintado, J., Murado, M.A., Gonzales, M.P., Miron, J., Pastrana, L., 1993. Joint effet of nitrogen and phosphorous concentrations on citric acid production by different strains of Aspergillus niger. Biotechnol. Lett. 15, 1157-1162.
15. del Rosario, E.J., Wong, R.L., 1984. Conversion of dextrinized cassava strach into ethanol using cultures of Aspergillus awamori and Saccharomyces cerevisiae. Enzyme Microb. Technol. 6, 60-64.
16. Schmidt, O., Angermann, H., Frommhold-Treu, I., Hoppe, K., 1995. Experimental and theoretical investigations of submerged fermentation and synthesis of pectinolytic enzymes by Aspergillus niger. Appl. Mirobiol. Biotechnol. 43, 424-430.
17. Schmidt, W.J., Kuhlmann, W., Schugerl, K., 1984. Automated determination of glucose in fermentation broths with p-hydroxy-benzoic-acid hydrazide (p-HBAH). Appl. Mirobiol. Biotechnol. 21, 78-84.
18. Siedenberg, D., Gerlach, S.R., Schugerl, K., Giuseppin, M., Hunik, J., 1997. Production of xylanase by Aspergillus awamori in synthetic medium in shaked flask cultures, submitted.
19. Singh, A., Agrawal, A.K., Abidi, A.B., Darmwal, N.S., 1990. Properties of cellobiase from Aspergillus niger. Appl. Microbiol. Biotechnol. 34, 356-358.
20. Smith, B.C., Wood, T.M., 1991. Xylanase production by Aspergillus awamori. Development of a medium and optimization of the fermentation parameters for the production of extracellular xylanase and β-glucosidase, while maintaining low protease production. Biotechnol. Bioeng. 38, 883-890.
21. Stoscheck, M.R., 1990. Quantitation of protein, general methods for handling proteins and enzymes. Methods. Enzymol. 182, 62-63.
22. Weigel, B., Hitzmann, B., Kretzmer, G., Schugerl, K., Huwing, A., Giffhorn, F., 1996. Analysis of various sugars by means of immobilized enzyme coupled flow injection analysis (FIA). J. Biotechnol. 50, 93-106.
23. Yigitoglu, M., McNeil, B., 1992. Ammonium ion and citric acid supplementation in batch cultures of Aspergillus niger B60. Biotechnol. Lett. 14, 831-836.