Cultivation of Aspergillus awamori on wheat bran: Ultrastructural description of growth and localization of xylanase by immunogold labeling

Journal of Biotechnology

Volumn 46, Issue 3, 1996, Pages 221-234

  Adolph, Stefan ^a   Müller, Susanne ^a   Siedenberg, Detlef ^a   Jäger, Karin ^a   Lehmann, Heiner ^a   Schügerl, Karl ^a   Giuseppin, Marco ^b  

^a LEIBNIZ UNIVERSITY HANNOVER   (Germany)

^b UNILEVER RESEARCH VLAARDINGEN   (Netherlands)

Author keywords

Aspergillus awamori; electron energy loss spectroscopy (EELS); electron spectroscopic imaging (ESI); immunogold labeling; transmission electron microscopy (TEM); ultrastructure; xylanase

Indexed keywords

XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; ASPERGILLUS; CONTROLLED STUDY; ENZYME LOCALIZATION; FUNGUS CULTURE; FUNGUS GROWTH; IMMUNOGOLD STAINING; NONHUMAN; PRIORITY JOURNAL; ULTRASTRUCTURE; WHEAT;

EID: 0342576234     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-1656(95)00198-0     Document Type: Article

References (23)

1. Bauer, R. (1988) Electron spectroscopic imaging: an advanced technique for imaging and analysis in transmission electron microscopy. Meth. Microbiol. 20, 113-146.
2. Belozerskaya, T.A. and Potapova, T.V. (1993) Intrahyphal communication in segmented mycelium. Exp. Mycol. 17, 157-169.
3. Biely, P. (1985) Microbial xylanolytic systems. Trends Biotechnol. 3(11), 286-290.
4. Bradbury, D., MacMasters, M.M. and Cull, I.M. (1956) Structure of the mature wheat kernel. II. Microscopic structure of pericarp, seed coat and other coverings of the endosperm and germ of hard red winter wheat. Cereal Chem. 33, 342-361; (1956b) III. Microscopic structure of endosperm of hard winter wheat. Cereal Chem. 33, 367-373.
5. Bradbury, D., MacMasters, M.M. and Cull, I.M. (1956) Structure of the mature wheat kernel. II. Microscopic structure of pericarp, seed coat and other coverings of the endosperm and germ of hard red winter wheat. Cereal Chem. 33, 342-361; (1956b) III. Microscopic structure of endosperm of hard winter wheat. Cereal Chem. 33, 367-373.
6. Buller, A.H.R. (1958) Further Observations on the Coprini Together With Some Investigations on Social Organisation and Sex in Hymenomycetes, Researches in Fungi, Vol. 4. Hafner Publishing Co., New York.
7. Fiddy, C. and Trinci, A.P.J. (1976) Mitosis, septation and branching in the dublication cycle in Aspergillus nidulans. J. Gen. Microbiol. 97, 169-184.
8. Collinge, A.J. and Trinci, A.P.J. (1974) Hyphal tips of wild-type and spreading colonial mutants of Neurospora crassa. Arch. Mikrobiol. 99, 358-368.
9. Grove, S.N. and Bracker, C.E. (1970) Protoplasmatic organisation of hyphal tips among fungi: Vesicles and Spitzenkörper. J. Bacteriol. 104(2), 989-1009.
10. Jäger, K.M. and Bergman, B. (1991) Localization of a multifunctional chaperonin (GroEL protein) in nitrogen fixing Anabaena PCC 7120. Presence in vegetative cells and heterocysts. Planta 183, 120-125.
11. Kualaev, I.S. and Vagabov, M. (1983) Polyphosphatemetabolisms in microorganisms. Adv. Microbiol. Physiol. 24, 83-158.
12. McClure, W.K., Park, D. and Robinson, P.M. (1968) Apical organisation in the somatic hyphae of fungi. J. Gen. Microbiol. 50, 177-182.
13. Miller, C.V. and Anderson, N.A. (1961) Proliferation of conidophores and intrahyphal hyphae in Aspergillus niger. Mycologia 57, 433-436.
14. Probst, W. and Bauer, R. (1987) Technique and biological application of the electron spectroscopic imaging (ESI) method and the electron energy-loss spectroscopy (EELS). Verh. Deutsch. Zool. Ges. 80, 119-128
15. Ouchterlony, Ö. (1958) Diffusion-in-gel methods for immunological analysis, In: Kallos, P. (Ed.), Progress in Allergy, Vol. V. Karger, Basel, pp. 1-78.
16. Reese, E.T., Maguire, A. and Parrish, F.W., (1973) Production of β-D-xylopyranosidases by fungi. Can. J. Microbiol. 19, 1065-1074.
17. Reynolds, E.S. (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208-212.
18. Roth, J. (1982) The protein A-gold (pAg) technique-a qualitative and quantitative approach for antigen localization on thin sections, In: Bullock, G.R. and Petrusz, P. (Eds.) Techniques in Immunocytochemistry, Vol. 1. Academic Press, London, pp. 107-133
19. Siedenberg, D. (1996) Dissertation, University of Hannover, Hannover, Germany.
20. Sprey, B. (1988) Cellular and extracellular localization of endocellulase in Trichoderma reesei. FEMS Microbiol. Lett. 55, 283-294.
21. Srebotnik, E., Messner, K. and Foisner, R. (1988) Ultrastructural localization of ligninase of Phanerochaete chrysosporium by immunogold labeling. Current Microbiol. 16, 221-227.
22. Tanaka, K. and Yanagita, T. (1963) Electron microscopy on ultrathin sections of Aspergillus niger. J. Gen. Appl. Microbiol. 9(1), 101-118.
23. Trinci, A.P.J. (1978) The dublication cycle and vegetative development in moulds. In: Smith, J.E. and Berry, D.R. (Eds.), The filamentous fungi, Vol. 3. Edward Arnold, pp. 133-163.