Rapid screening for endo-β-1,4-glucanase and endo-β-1,4-mannanase activities and specific measurement using soluble dye-labelled substrates

Journal of Microbiological Methods

Volumn 29, Issue 1, 1997, Pages 15-21

  Fülöp, László ^a   Ponyi, Tamás ^a  

^a SZENT ISTVÁN UNIVERSITY   (Hungary)

Author keywords

Cellulase; Cellulomonas sp.; Mannanase; Screening

Indexed keywords

BETA MANNOSIDASE; CELLULASE;

ARTICLE; BACTERIUM IDENTIFICATION; CELLULOMONAS; ENZYME ASSAY; ENZYME LOCALIZATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; IMMUNOBLOTTING; NONHUMAN; PRIORITY JOURNAL;

EID: 0342601915     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(97)00984-6     Document Type: Article

References (11)

1. Béguin P. (1983) Detection of cellulase activity in polyacrylamide gels using Congo Red-stained agar replicas. Anal. Biochem. 131, 333-336.
2. Gilbert, H.J., Hazlewood, G.P., Laurie, J.I., Orpin, C.G. and Xue, G.P. (1992) Homologous catalytic domains in a rumen fungal xylanase: evidence for gene duplication and prokaryotic origin. Mol. Microbiol. 6, 2065-2076.
3. Millward-Sadler, S.J., Poole, D.M., Henrissat, B., Hazlewood, G.P., Clarke, J.H. and Gilbert, H.J. (1994) Evidence for a general role for high-affinity non-catalytic cellulose binding domains in microbial plant cell wall hydrolases. Mol. Microbiol. 11, 375-382.
4. Fanutti, C., Ponyi, T., Black, G.W., Hazlewood, G.P. and Gilbert, H.J. (1995) The conserved noncatalytic 40-residue sequence in cellulases and hemicellulases from Anaerobic fungi functions as a protein docking domain. J. Biol. Chem. 270, 29314-29322.
5. McCleary, B.V. (1988) Soluble, dye-labelled polysaccharides for assay of endohydrolases. Meth. Enzymol. 160, 74-86.
6. Miller, G.L. (1959) Use of dinitrosalicylic reagent for determination of reducing sugars. Anal. Chem. 31, 426-428.
7. Biely, P., Mislovicova, D. and Toman, R. (1985) Soluble chromogenic substrates for the assay of endo-1,4-beta-xylanases and endo-1,4,beta-glucanases. Anal. Biochem. 144, 142-146.
8. Deshpande, M.V., Eriksson, K.E. and Pettersson, L.G. (1984) An assay for selective determination of exo-1,4-beta-glucanases in a mixture of cellulolytic enzymes. Anal. Biochem. 138, 481-487.
9. Stewart, B.J. and Leatherwood, J.M. (1976) Depressed synthesis of cellulase by Cellulomonas. J. Bacteriol. 128, 609-615.
10. Fülöp, L., Trân, S.L.P., Prágai, Z., Felföldi, F. and Ponyi, T. (1996) Cloning and expression of a β-1,4-endo-glucanase gene from Cellulomonas sp. Ce1B7 in Escherichia coli, purification and characterization of the recombinant enzyme. FEMS. Lett. 145, 355-360.
11. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.