Cloning and expression of a β-1,4-endoglucanase gene from Cellulomonas sp. CelB7 in Escherichia coli; purification and characterization of the recombinant enzyme

FEMS Microbiology Letters

Volumn 145, Issue 3, 1996, Pages 355-360

  Fülöp, László ^a   Trân, Son Lam Phan ^a   Prágai, Zoltán ^a   Felföldi, Ferenc ^b   Ponyi, Tamás ^a  

^a SZENT ISTVÁN UNIVERSITY   (Hungary)

^b INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

Author keywords

1,4 endoglucanase; cellulomonas; cloning; enzyme assay; purification

Indexed keywords

CELLULOSE; GLUCAN SYNTHASE; LIGNIN; XYLAN;

ARTICLE; CELLULOMONAS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; FAST PROTEIN LIQUID CHROMATOGRAPHY; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL;

ACTINOMYCETALES; BETA-GLUCOSIDASE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE LIBRARY; GENES, BACTERIAL; HYDROGEN-ION CONCENTRATION; MOLECULAR WEIGHT; RECOMBINANT PROTEINS; RESTRICTION MAPPING; SUBSTRATE SPECIFICITY; TEMPERATURE;

CELLULOMONAS; CELLULOMONAS SP.; ESCHERICHIA COLI;

EID: 0030589475     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(96)00431-4     Document Type: Article

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