The use of 16s and 16s-23s rrna internal transcribed spacers to detect and differentiate erwinia amylovora

Acta Horticulturae

Volumn 489, Issue , 1999, Pages 49-54

  Jeng, R S ^a   Beliaeva, L ^a   Hubbes, M ^a   Svircev, A M ^b   Myers, A L ^b  

^a UNIVERSITY OF TORONTO   (Canada)

^b AGRICULTURE AND AGRI FOOD CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0242715595     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/ActaHortic.1999.489.4     Document Type: Conference Paper

References (7)

1. Bereswill, S., Pahi, A., Belleman, P., Zeller, W. and Geiger, K. 1992. Sensitive and species-specific detection of Erwinia amylovora by PCR-analysis. Appi. Environ. Microbiol. 58: 3522-3526.
2. Gurtler, V. and Stanisich, V. A. 1996. New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology 142: 3-16.
3. Kwon, S. W., Go, S. J., Kang, H. W., Ryu, J. C. and Jo, J. K. 1997. Phylogenetic analysis of Erwinia species based on 1 6S rRNA gene sequences. International Journal of Systematic Bacteriology 47(4): 1061-1067.
4. McManus, P.S. and Jones, A.L. 1995a. Detection of Erwinia amylovora by nested PCR and PCR-dot-blot and reverse-blot hybridizations. Phytopath. 85: 6 18-623.
5. McManus, P.S. and Jones, A..L. 1995b. Genetic fingerprinting of Erwinia amylovora strains isolated from tree-fruit crops and Rubus spp. Phytopath. 35: 1547-1553.
6. Momol, M. T., Momol, E. A., Lamboy, W. F., Norell, J. L., Beer, S. V. and Aldwinckle, H.S. 1997. Characterization of Erwinia amylovora strains using random amplified polymorphic DNA fragments (RAPD5). Journal of Applied Microbiology. 82: 389-398.
7. Tyler, S. D., Strathdee, C. A., Rozee, K. R. and Johnson, W. M. 1995 Oligonucleotide primers designed to differentiate pathogenic Pseudomonads on the basis of the sequencing of genes coding for 6S-23S rRNA internal transcribed spacers. Clinical and Diagnostic Laboratory Immunology 2(4): 448-453.