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Volumn 107, Issue 2, 2000, Pages 251-261

Targeted integration into a rRNA locus results in uniform and high level expression of transgenes in Leishmania amastigotes

Author keywords

EGFP; Leishmania major; Leishmania mexicana; Persistence; GAL

Indexed keywords

RIBOSOME RNA;

EID: 0242693036     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(00)00195-X     Document Type: Article
Times cited : (127)

References (29)
  • 1
    • 0025650360 scopus 로고
    • Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes
    • LeBowitz J.H., Coburn C.M., McMahon-Pratt D. et al. Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes. Proc. Natl. Acad. Sci. USA. 87:1990;9736-9740.
    • (1990) Proc. Natl. Acad. Sci. USA , vol.87 , pp. 9736-9740
    • Lebowitz, J.H.1    Coburn, C.M.2    McMahon-Pratt, D.3
  • 2
    • 0343267840 scopus 로고    scopus 로고
    • Use of the green fluorescent protein as a marker in transfected Leishmania
    • Ha D.S., Schwarz J.K., Turco S.J. et al. Use of the green fluorescent protein as a marker in transfected Leishmania. Mol. Biochem. Parasitol. 77:1996;57-64.
    • (1996) Mol. Biochem. Parasitol. , vol.77 , pp. 57-64
    • Ha, D.S.1    Schwarz, J.K.2    Turco, S.J.3
  • 3
    • 0029840937 scopus 로고    scopus 로고
    • Antigen presentation by Leishmania mexicana-infected macrophages: Activation of helper T cells by a model parasite antigen secreted into the parasitophorous vacuole or expressed on the amastigote surface
    • Wolfram M., Fuchs M., Wiese M. et al. Antigen presentation by Leishmania mexicana-infected macrophages: activation of helper T cells by a model parasite antigen secreted into the parasitophorous vacuole or expressed on the amastigote surface. Eur. J. Immunol. 26:1996;3153-3162.
    • (1996) Eur. J. Immunol. , vol.26 , pp. 3153-3162
    • Wolfram, M.1    Fuchs, M.2    Wiese, M.3
  • 4
    • 0033198238 scopus 로고    scopus 로고
    • Genetic manipulation of kinetoplastida
    • Clayton C.E. Genetic manipulation of kinetoplastida. Parasitol. Today. 15:1999;372-378.
    • (1999) Parasitol. Today , vol.15 , pp. 372-378
    • Clayton, C.E.1
  • 5
    • 0026000950 scopus 로고
    • RNA polymerase I can mediate expression of CAT and neo protein-coding genes in Trypanosoma brucei
    • Rudenko G., Chung H.M., Pham V.P. et al. RNA polymerase I can mediate expression of CAT and neo protein-coding genes in Trypanosoma brucei. EMBO J. 10:1991;3387-3397.
    • (1991) EMBO J. , vol.10 , pp. 3387-3397
    • Rudenko, G.1    Chung, H.M.2    Pham, V.P.3
  • 6
    • 0025994368 scopus 로고
    • Alpha-amanitin-resistant transcription units in trypanosomes: A comparison of promoter sequences for a VSG gene expression site and for the ribosomal RNA genes
    • Zomerdijk J.C., Kieft R., Shiels P.G. et al. Alpha-amanitin-resistant transcription units in trypanosomes: a comparison of promoter sequences for a VSG gene expression site and for the ribosomal RNA genes. Nucleic Acids Res. 19:1991;5153-5158.
    • (1991) Nucleic Acids Res. , vol.19 , pp. 5153-5158
    • Zomerdijk, J.C.1    Kieft, R.2    Shiels, P.G.3
  • 8
    • 0028834777 scopus 로고
    • Increased expression of LD1 genes transcribed by RNA polymerase I in Leishmania donovani as a result of duplication into the rRNA gene locus
    • Lodes M.J., Merlin G., deVos T. et al. Increased expression of LD1 genes transcribed by RNA polymerase I in Leishmania donovani as a result of duplication into the rRNA gene locus. Mol. Cell. Biol. 15:1995;6845-6853.
    • (1995) Mol. Cell. Biol. , vol.15 , pp. 6845-6853
    • Lodes, M.J.1    Merlin, G.2    DeVos, T.3
  • 9
    • 0029897406 scopus 로고    scopus 로고
    • The promoter for the ribosomal RNA genes of Leishmania chagasi
    • Gay L.S., Wilson M.E., Donelson J.E. The promoter for the ribosomal RNA genes of Leishmania chagasi. Mol. Biochem. Parasitol. 77:1996;193-200.
    • (1996) Mol. Biochem. Parasitol. , vol.77 , pp. 193-200
    • Gay, L.S.1    Wilson, M.E.2    Donelson, J.E.3
  • 10
    • 0029881485 scopus 로고    scopus 로고
    • Structural and functional characterization of the Leishmania amazonensis ribosomal RNA promoter
    • Uliana S.R., Fischer W., Stempliuk V.A. et al. Structural and functional characterization of the Leishmania amazonensis ribosomal RNA promoter. Mol. Biochem. Parasitol. 76:1996;245-255.
    • (1996) Mol. Biochem. Parasitol. , vol.76 , pp. 245-255
    • Uliana, S.R.1    Fischer, W.2    Stempliuk, V.A.3
  • 11
    • 0343416375 scopus 로고    scopus 로고
    • PRIBOTEX expression vector: A pTEX derivative for a rapid selection of Trypanosoma cruzi transfectants
    • Martinez-Calvillo S., Lopez I., Hernandez R. pRIBOTEX expression vector: a pTEX derivative for a rapid selection of Trypanosoma cruzi transfectants. Gene. 199:1997;71-76.
    • (1997) Gene , vol.199 , pp. 71-76
    • Martinez-Calvillo, S.1    Lopez, I.2    Hernandez, R.3
  • 12
    • 0025339650 scopus 로고
    • Trans splicing of nuclear pre-mRNAs
    • Agabian N. Trans splicing of nuclear pre-mRNAs. Cell. 61:1990;1157-1160.
    • (1990) Cell , vol.61 , pp. 1157-1160
    • Agabian, N.1
  • 13
    • 0025805653 scopus 로고
    • Maturation of polycistronic pre-mRNA in Trypanosoma brucei: Analysis of trans splicing and poly(A) addition at nascent RNA transcripts from the hsp70 locus
    • Huang J., Van der Ploeg L.H. Maturation of polycistronic pre-mRNA in Trypanosoma brucei: analysis of trans splicing and poly(A) addition at nascent RNA transcripts from the hsp70 locus. Mol. Cell. Biol. 11:1991;3180-3190.
    • (1991) Mol. Cell. Biol. , vol.11 , pp. 3180-3190
    • Huang, J.1    Van Der Ploeg, L.H.2
  • 14
    • 0026085421 scopus 로고
    • Transient activity assays of the Trypanosoma brucei variant surface glycoprotein gene promoter: Control of gene expression at the post-transcriptional level
    • Jefferies D., Tebabi P., Pays E. Transient activity assays of the Trypanosoma brucei variant surface glycoprotein gene promoter: control of gene expression at the post-transcriptional level. Mol. Cell. Biol. 11:1991;338-343.
    • (1991) Mol. Cell. Biol. , vol.11 , pp. 338-343
    • Jefferies, D.1    Tebabi, P.2    Pays, E.3
  • 15
    • 0029043576 scopus 로고
    • Trypanosoma brucei: A preferential splicing at the inverted polyadenylation site of the VSG mRNA provides further evidence for coupling between trans-splicing and polyadenylation
    • Berberof M., Vanhamme L., Pays E. Trypanosoma brucei: a preferential splicing at the inverted polyadenylation site of the VSG mRNA provides further evidence for coupling between trans-splicing and polyadenylation. Exp. Parasitol. 80:1995;563-567.
    • (1995) Exp. Parasitol. , vol.80 , pp. 563-567
    • Berberof, M.1    Vanhamme, L.2    Pays, E.3
  • 16
    • 0029012720 scopus 로고
    • Intergenic regions between tandem gp63 genes influence the differential expression of gp63 RNAs in Leishmania chagasi promastigotes
    • Ramamoorthy R., Swihart K.G., McCoy J.J. et al. Intergenic regions between tandem gp63 genes influence the differential expression of gp63 RNAs in Leishmania chagasi promastigotes. J. Biol. Chem. 270:1995;12133-12139.
    • (1995) J. Biol. Chem. , vol.270 , pp. 12133-12139
    • Ramamoorthy, R.1    Swihart, K.G.2    McCoy, J.J.3
  • 17
    • 0030035450 scopus 로고    scopus 로고
    • The developmental expression of Leishmania donovani A2 amastigote-specific genes is post-transcriptionally mediated and involves elements located in the 3′-untranslated region
    • Charest H., Zhang W.W., Matlashewski G. The developmental expression of Leishmania donovani A2 amastigote-specific genes is post-transcriptionally mediated and involves elements located in the 3′-untranslated region. J. Biol. Chem. 271:1996;17081-17090.
    • (1996) J. Biol. Chem. , vol.271 , pp. 17081-17090
    • Charest, H.1    Zhang, W.W.2    Matlashewski, G.3
  • 18
    • 0032169005 scopus 로고    scopus 로고
    • The 3′ untranslated region of the hsp 70 genes maintains the level of steady state mRNA in Trypanosoma brucei upon heat shock
    • Lee M.G. The 3′ untranslated region of the hsp 70 genes maintains the level of steady state mRNA in Trypanosoma brucei upon heat shock. Nucleic Acids Res. 26:1998;4025-4033.
    • (1998) Nucleic Acids Res. , vol.26 , pp. 4025-4033
    • Lee, M.G.1
  • 19
    • 0030970429 scopus 로고    scopus 로고
    • The multiple cpb cysteine proteinase genes of Leishmania mexicana encode isoenzymes that differ in their stage regulation and substrate preferences
    • Mottram J.C., Frame M.J., Brooks D.R. et al. The multiple cpb cysteine proteinase genes of Leishmania mexicana encode isoenzymes that differ in their stage regulation and substrate preferences. J. Biol. Chem. 272:1997;14285-14293.
    • (1997) J. Biol. Chem. , vol.272 , pp. 14285-14293
    • Mottram, J.C.1    Frame, M.J.2    Brooks, D.R.3
  • 20
    • 0025871685 scopus 로고
    • Simultaneous transient expression assays of the trypanosomatid parasite Leishmania using β-galactosidase and β-glucuronidase as reporter enzymes
    • LeBowitz J.H., Coburn C.M., Beverley S.M. Simultaneous transient expression assays of the trypanosomatid parasite Leishmania using β-galactosidase and β-glucuronidase as reporter enzymes. Gene. 103:1991;119-123.
    • (1991) Gene , vol.103 , pp. 119-123
    • Lebowitz, J.H.1    Coburn, C.M.2    Beverley, S.M.3
  • 21
    • 0025882658 scopus 로고
    • Double targeted gene replacement for creating null mutants
    • Cruz A., Coburn C.M., Beverley S.M. Double targeted gene replacement for creating null mutants. Proc. Natl. Acad. Sci. USA. 88:1991;7170-7174.
    • (1991) Proc. Natl. Acad. Sci. USA , vol.88 , pp. 7170-7174
    • Cruz, A.1    Coburn, C.M.2    Beverley, S.M.3
  • 22
    • 0029973636 scopus 로고    scopus 로고
    • FACS-optimized mutants of the green fluorescent protein (GFP)
    • Cormack B.P., Valdivia R.H., Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene. 173:1996;33-38.
    • (1996) Gene , vol.173 , pp. 33-38
    • Cormack, B.P.1    Valdivia, R.H.2    Falkow, S.3
  • 23
    • 0026080548 scopus 로고
    • Genetic control of the immune response in mice to Leishmania mexicana surface protease
    • Lopez J.A., Reins H.A., Etges R.J. et al. Genetic control of the immune response in mice to Leishmania mexicana surface protease. J. Immunol. 146:1991;1328-1334.
    • (1991) J. Immunol. , vol.146 , pp. 1328-1334
    • Lopez, J.A.1    Reins, H.A.2    Etges, R.J.3
  • 24
    • 0020581961 scopus 로고
    • Isolation and characterization of infective and non-infective clones of Leishmania tropica
    • Handman E., Hocking R.E., Mitchell G.F. et al. Isolation and characterization of infective and non-infective clones of Leishmania tropica. Mol. Biochem. Parasitol. 7:1983;111-126.
    • (1983) Mol. Biochem. Parasitol. , vol.7 , pp. 111-126
    • Handman, E.1    Hocking, R.E.2    Mitchell, G.F.3
  • 25
    • 0027475492 scopus 로고
    • Leishmania mexicana promastigotes induce cytotoxic T lymphocytes in vivo that do not recognize infected macrophages
    • Lopez J.A., LeBowitz J.H., Beverley S.M. et al. Leishmania mexicana promastigotes induce cytotoxic T lymphocytes in vivo that do not recognize infected macrophages. Eur. J. Immunol. 23:1993;217-223.
    • (1993) Eur. J. Immunol. , vol.23 , pp. 217-223
    • Lopez, J.A.1    Lebowitz, J.H.2    Beverley, S.M.3
  • 26
    • 0026674542 scopus 로고
    • Axenic cultivation and characterization of Leishmania mexicana amastigote-like forms
    • Bates P.A., Robertson C.D., Tetley L. et al. Axenic cultivation and characterization of Leishmania mexicana amastigote-like forms. Parasitology. 105:1992;193-202.
    • (1992) Parasitology , vol.105 , pp. 193-202
    • Bates, P.A.1    Robertson, C.D.2    Tetley, L.3
  • 27
    • 0027931677 scopus 로고
    • Surface antigens of Leishmania mexicana amastigotes: Characterization of glycoinositol phospholipids and a macrophage-derived glycosphingolipid
    • Winter G., Fuchs M., McConville M.J. et al. Surface antigens of Leishmania mexicana amastigotes: characterization of glycoinositol phospholipids and a macrophage-derived glycosphingolipid. J. Cell. Sci. 107:1994;2471-2482.
    • (1994) J. Cell. Sci. , vol.107 , pp. 2471-2482
    • Winter, G.1    Fuchs, M.2    McConville, M.J.3
  • 28
    • 0027377867 scopus 로고
    • Application of LacZ gene fusions to post-implantation development
    • Bonnerot C., Nicolas J.F. Application of LacZ gene fusions to post-implantation development. Meth. Enzymol. 225:1993;451-469.
    • (1993) Meth. Enzymol. , vol.225 , pp. 451-469
    • Bonnerot, C.1    Nicolas, J.F.2
  • 29
    • 0032583153 scopus 로고    scopus 로고
    • Genetic nomenclature for Trypanosoma and Leishmania
    • Clayton C.E., Adams M., Almeida R. et al. Genetic nomenclature for Trypanosoma and Leishmania. Mol. Biochem. Parasitol. 97:1998;221-224.
    • (1998) Mol. Biochem. Parasitol. , vol.97 , pp. 221-224
    • Clayton, C.E.1    Adams, M.2    Almeida, R.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.