Separation and quantification of cellulases and hemicellulases by capillary electrophoresis

Analytical Biochemistry

Volumn 317, Issue 1, 2003, Pages 85-93

  Jørgensen, Henning ^a   Kutter, Jörg P ^a   Olsson, Lisbeth ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

1,3 diaminopropane; Adsorption; Cellobiohydrolase; Endoglucanase; Endoxylanase; Penicillium brasilianum; Protein analysis

Indexed keywords

ADSORPTION; CAPILLARY ELECTROPHORESIS; FUNGI; FUSED SILICA; SEPARATION;

1 ,3-DIAMINOPROPANE; CELLOBIOHYDROLASES; ENDOGLUCANASES; ENDOXYLANASE; PENICILLIUM BRASILIANUM; PROTEIN ANALYSIS;

ENZYMES;

1,3 PROPANEDIAMINE; ALKANE DERIVATIVE; CELLULASE; CELLULOSE 1,4 BETA CELLOBIOSIDASE; ENDOXYLANASE; GLUCAN SYNTHASE; HEMICELLULASE; ISOENZYME; SILICON DIOXIDE; UNCLASSIFIED DRUG;

ADSORPTION; ARTICLE; CAPILLARY ELECTROPHORESIS; CONCENTRATION (PARAMETERS); ENZYME ASSAY; MATERIAL COATING; PH; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; SEPARATION TECHNIQUE;

FUNGI; PENICILLIUM; PENICILLIUM BRASILIANUM;

EID: 0242669397     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(03)00052-6     Document Type: Article

References (43)

1. Wood T.M. Properties of cellulolytic enzyme systems. Biochem. Soc. Trans. 13:1985;407-410.
2. Beg Q.K., Kapoor M., Mahajan L., Hoondal G.S. Microbial xylanases and their industrial applications: a review. Appl. Microbiol. Biotechnol. 56:2001;326-338.
3. Kubicek C.P., Messner R., Gruber F.M.R.L., Kubicek-Pranz E. The Trichoderma cellulase regulatory puzzle: from the interior life of a secretory fungus. Enzyme Microb. Technol. 15:1993;90-99.
4. Xu J., Takakuwa N., Nogawa M., Okada H., Morikawa Y. A third xylanase from Trichoderma reesei PC-3-7. Appl. Microbiol. Biotechnol. 49:1998;718-724.
5. Sandgren M., Shaw A., Ropp T.H., Wu S., Bott R., Cameron A.D., Stahlberg J., Mitchinson C., Jones T.A. The X-ray crystal structure of the Trichoderma reesei family12 endoglucanase 3, Cel12A, at 1.9. Å resolution J. Mol. Biol. 308:2001;295-310.
6. Mandels M., Andreotti R., Roche C. Measurement of saccharifying cellulase. Biotechnol. Bioeng. Symp. 6:1976;21-33.
7. Ghose T. Measurement of Cellulase Activities. 1984;Commission on Biotechnology, International Union of Pure and Applied Chemistry, Biochemical Engineering Research Centre, IIT, Delhi.
8. Wood T.M., Bhat K.M. Colowick S.P. Biomass - A: Cellulose and Hemicellulose. 1988;87-112 Academic Press, San Diego.
9. Biely P., Mislovicova D., Toman R. Soluble chromogenic substrates for the assay of endo-1,4-β-xylanases and endo-1,4-β-glucanases. Anal. Biochem. 144:1985;142-146.
10. Baraznenok V.A., Ankudimova N.V., Sinitsyn A.P., Popava N.N., Gutyerres B., Okunev O.N., Zorov I.N. Comparison of cellulase activity assays with stained and unstained cellulose derivatives. Biochemistry (Moscow). 62:1997;760-766.
11. Deshpande M.V., Eriksson K.-E., Pettersson L.G. An assay for selective determination of exo-1,4,-β-glucanases in a mixture of cellulolytic enzymes. Anal. Biochem. 138:1984;481-487.
12. Nidetzky B., Claeyssens M. Specific quantification of Trichoderma reesei cellulases in reconstituted mixtures and its application to cellulase-cellulose binding studies. Biotechnol. Bioeng. 44:1994;961-966.
13. Biely P., Vrsanská M., Tenkanen M., Kluepfel D. Endo-β-1,4-xylanase families: differences in catalytic properties. J. Biotechnol. 57:1997;151-166.
14. Kolbe J., Kubicek C.P. Quantification and identification of the main components of the Trichoderma cellulase complex with monoclonal antibodies using an enzyme-linked immunosorbent assay (ELISA). Appl. Microbiol. Biotechnol. 34:1990;26-30.
15. Vinznat T.B., Adney W.S., Decker S.R., Baker J.O., Kinther M.T., Sherman N.E., Fox J.W., Himmel M.E. Fingerprinting Trichoderma reesei hydrolases in a commercial cellulase preparation. Appl. Biochem. Biotechnol. 91-93:2001;99-107.
16. Yu A.H.C., Lee D., Saddler J.N. A quantitative approach to the study of the adsorption/desorption of cellulase components in a crude cellulase mixture. Biotechnol. Tech. 7:1993;713-718.
17. Medve J., Lee D., Tjerneld F. Ion-exchange chromatographic purification and quantitative analysis of Trichoderma reesei cellulases cellobiohydrolase I, II and endoglucanase II by fast protein liquid chromatography. J. Chromatogr. A. 808:1998;153-165.
18. Oda R.P., Madden B.J., Spelsberg T.C., Landers J.P. α,ω -Bis-quaternary ammonium alkanes as effective buffer additives for enhanced capillary electrophoretic separation of glycoproteins J. Chromatogr. A. 680:1994;85-92.
19. Wehr T., Rodriguez-Diaz R., Zhu M. Capillary Electrophoresis of Proteins. 1999;Marcel Dekker Inc. New York, Basel, Hong Kong.
20. Strege M.A., Lagu A.L. Capillary electrophoretic separation of biotechnology derived protein in E. coli fermentation broth. Electrophoresis. 16:1995;642-646.
21. Frazier R.A., Ames J.M., Nursten H.E. The development and application of capillary electrophoresis methods for food analysis. Electrophoresis. 20:1999;3156-3180.
22. Luguera C., Moreno-Arribas V., Pueyo E., Polo M.C. Capillary electrophoretic analysis of wine proteins. Modifications during the manufacture of sparkling wines. J. Agric. Food Chem. 45:1999;3766-3770.
23. Recio I., Ramos M., Lopez-Fandino R. Capillary electrophoresis for the analysis of food proteins of animal origin. Electrophoresis. 22:2001;1489-1502.
24. Strege M.A., Schmidt D.F., Kreuzman A., Dotzlaf J., Yeh W.K., Kaiser R.E., Lagu A.L. Capillary electrophoretic analysis of serine hydroxymethyltransferase in Escherichia coli fermentation broth. Anal. Biochem. 223:1994;198-204.
25. McNerney T.M., Watson S.K., Sim J., Bridenbaugh R.L. Separation of recombinant human growth hormone from Escherichia coli cell pellet extract by capillary zone electrophoresis. J. Chromatogr. A. 744:1996;223-229.
26. Song L., Ou Q., Yu W. Study on phosphate of ethylenediamine,1,3-diaminopropane and 1,4-diaminopropane as carrying electrolyte in open-tubular capillary electrophoresis. J. Liq. Chromatogr. 17:1994;1953-1969.
27. Giordano B.C., Muza M., Trout A., Landers J.P. Dynamically-coated capillaries allow for capillary electrophoretic resolution of transferrin sialoforms via direct analysis of human serum. J. Chromatogr. B. 742:2000;79-89.
28. Oda R.P., Landers J.P. Application of capillary electrophoresis to the analysis of proteins. Bioseparations. 5:1995;315-328.
29. Morbeck D.E., Madden B.J., McCormick D.J. Analysis of the microheterogeneity of the glycoprotein chorionic gonadotropin with high-performance capillary electrophoresis. J. Chromatogr. A. 680:1994;217-224.
30. Koivula A., Lappalainen A., Virtanen S., Mäntylä A.L., Suominen P., Teeri T.T. Immunoaffinity chromatographic purification of cellobiohydrolase II mutants from recombinant Trichoderma reesei strains devoid of major endoglucanase genes. Protein Expr. Purif. 8:1996;391-400.
31. H. Jørgensen, T. Eriksson, J. Börjesson, F. Tjerneld, L. Olsson, Purification and characterization of five cellulases and one xylanase from Penicillium brasilianum IBT 20888, 2002, submitted for publication.
32. Mandels M., Weber J. The production of cellulases. Adv. Chem. Ser. 95:1969;394-414.
33. M. Carlsen, α-amylase production by Aspergillus oryzae. Ph.D. thesis, Technical University of Denmark, Lyngby, Denmark, 1994.
34. Lemos M.A., Teixeira J.A., Mota M., Gama F.M. A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease. Biotech. Lett. 22:2000;703-707.
35. Lauer H.H., McManigill D. Capillary zone electrophoresis of proteins in untreated fused silica tubing. Anal. Chem. 58:1986;166-170.
36. Klausen N.K., Kornfelt T. Analysis of the glycoforms of human recombinant factor VIIa by capillary electrophoresis and high-performance liquid chromatography. J. Chromatogr. A. 718:1995;195-202.
37. Steiner J., Socha C., Eyzaguirre J. Culture conditions for enhanced cellulase production by a native strain of Penicillium purpurogenum. World J. Microbiol. Biotechnol. 10:1994;280-284.
38. Ilmén M., Saloheimo A., Onnela M.L., Penttilä M.E. Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei. Appl. Environ. Microbiol. 63:1997;1298-1306.
39. Mernitz G., Koch A., Henrissat B., Schulz G. Endoglucanase II (EGII) of Penicillium janthinellum: cDNA sequence, heterologous expression and promotor analysis. Curr. Genet. 29:1996;490-495.
40. Teeri T.T., Koivula A. Cellulose degradation by native and engineered fungal cellulases. Carbohydrat. Eur. 12:1995;28-33.
41. Medve J., Karlsson J., Lee D., Tjerneld F. Hydrolysis of microcrystalline cellulose by cellobiohydrolase I and endoglucanase II from Trichoderma reesei: adsorption, sugar production pattern, and synergism of the enzymes. Biotechnol. Bioeng. 59:1998;621-634.
42. Medve J., Stahlberg J., Tjerneld F. Isotherms for adsorption of cellobiohydrolase I and II from Trichoderma reesei on microcrystalline cellulose. Appl. Biochem. Biotechnol. 66:1997;39-56.
43. Karlsson J., Medve J., Tjerneld F. Hydrolysis of steam-pretreated lignocellulose. Appl. Biochem. Biotechnol. 82:1999;243-258.