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Volumn 300, Issue 5619, 2003, Pages 653-656

Reversing the inactivation of peroxiredoxins caused by cysteine sulfinic acid formation

Author keywords

[No Author keywords available]

Indexed keywords

ACIDS; CELLS; ENZYMES; METABOLISM; OXIDATION;

EID: 0242668688     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1080273     Document Type: Article
Times cited : (486)

References (31)
  • 19
    • 0242486954 scopus 로고    scopus 로고
    • note
    • 51 of Prx I was determined to be sulfonic when analyzed by 2D gel electrophoresis and in-geldigestion, a procedure that exposes the denatured protein to air for a substantial period (13).
  • 22
    • 0242655370 scopus 로고    scopus 로고
    • note
    • 35S-labeled amino acids. The cells were finally rinsed twice with ice-cold HBSS and scraped into 1 ml of 10% trichloroacetic acid.
  • 23
    • 0242655371 scopus 로고    scopus 로고
    • note
    • 35S-labeled proteins in the 2D gels were visualized and quantified with the use of a FLA-3000 image analyzer (Fujifilm). For immunoblot analysis of Prx enzymes, proteins were transferred electrophoretically from 2D gels to a nitrocellulose membrane and probed with rabbit antibodies specific for Prx I or II. Immune complexes were detected with alkaline phosphatase conjugated secondary antibodies (KPL), CDP-Star (Tropix) chemi[uminescence reagent, and a Kodak Image Station 440.
  • 30
    • 0242571014 scopus 로고    scopus 로고
    • note
    • Materials and methods are available as supporting material on Science Online.
  • 31
    • 0242403607 scopus 로고    scopus 로고
    • note
    • We thank the members of H. Fales's laboratory for help with mass spectrometry. Supported by a Korean Science and Engineering Foundation Center of Excellence grant to the Center for Cell Signaling Research at Ewha Womans University and the Brain Korea 21 grant (to H.A.W. and S.W.K.), and by Korea Research Foundation Grant KRF-2001 (to H.Z.C.).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.