Viability of and Plasmid Retention in Frozen Recombinant Escherichia coli over Time: A Ten-Year Prospective Study

Applied and Environmental Microbiology

Volumn 69, Issue 11, 2003, Pages 6605-6609

  Koenig, Gina L ^a  

^a ROCHE MOLECULAR SYSTEMS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTICS; CELLS; ESCHERICHIA COLI;

PLASMID RETENTION;

MICROBIOLOGY;

AMPICILLIN; ANTIBIOTIC AGENT; CARBENICILLIN; METICILLIN;

ARTICLE; CELL VIABILITY; CONTROLLED STUDY; ESCHERICHIA COLI; NONHUMAN; PLASMID;

BACTERIOLOGICAL TECHNIQUES; CRYOPRESERVATION; DNA, RECOMBINANT; ESCHERICHIA COLI; PLASMIDS; PROSPECTIVE STUDIES; TIME FACTORS;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0242657574     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.11.6605-6609.2003     Document Type: Article

References (20)

1. Belt, A. 1996. Characterization of culture used for biotechnology and industry, p. 251-258. In J. C. Hunter-Cevera and A. Belt (ed.), Maintaining cultures for biotechnology and industry. Academic Press, New York, N.Y.
2. Dando, T., and I. Bousfield. 1991. Maintenance of industrial and marine bacteria and bacteriophages, p. 57-63. In B. Kirsop, and A. Doyle (ed.), Maintenance of industrial and marine bacteria and bacteriophages. Academic Press, New York, N.Y.
3. Dietz, A., and B. W. Churchill. 1985. Culture preservation and stability, p. 37-49. In A. T. Bull, and H. Dalton (ed.), Comprehensive biotechnology: the principles, applications and regulations of biotechnology in industry, agriculture and medicine, Pergamon Press, New York, N.Y.
4. Duncan, P. A. 2003. Characterization of microbial seeds used in the manufacture of biopharmaceuticals. BioProcess Int. 1:58-62.
5. Elion, E. 1997. Constructing recombinant DNA molecules by the polymerase chain reaction, p. 3.17. In F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.), Current protocols in molecular biology. John Wiley & Sons, New York, N.Y.
6. Fitzwater, T. 2002. Ligation and transformation protocol. U.S. Department of Commerce. [Online.] http://micro.nwfsc.noaa.gov/protocols/Lig_and_Trans.html.
7. Gherna, R. L. 1981. Preservation, p. 208-217. In P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg, and G. B. Phillips (ed.), Manual of methods for general bacteriology. American Society for Microbiology, Washington, D.C.
8. Heekly, R. J. 1978. Preservation of microorganisms, p. 1-53. In D. Perlman (ed.), Advances in applied microbiology. Academic Press, New York, NY.
9. Lawyer, F. C., S. Stoffel, R. K. Saiki, S. Y. Chang, P. A. Landre, R. D. Abramson, and D. H. Gelfand. 1993. High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5′ to 3′ exonuclease activity. PCR Methods Appl. 2:275-287.
10. Lawyer, F. C., S. Stoffel, R. K. Saiki, K. Myambo, R. Drummond, and D. H. Gelfand. 1989. Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus. J. Biol. Chem. 264:6427-6437.
11. Marquet, M., N. A. Horn, and J. A. Meek. 1997. Characterization of plasmid DNA vectors for use in human gene therapy, part 1. Biopharm 10:42-50.
12. Nierman, W., and T. Feldblyum. 1985. Cryopreservation of cultures that contain plasmids. Dev. Ind. Microbiol. 26:423-434.
13. Orle, K. A., C. A. Gates, D. H. Martin, B. A. Body, and J. B. Weiss. 1996. Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers. J. Clin. Microbiol. 34:49-54.
14. Pasloske, B. L., C. R. Walkerpeach, R. D. Obermoeller, M. Winkler, and D. B. DuBois. 1998. Armored RNA technology for production of ribonuclease-resistant viral RNA controls and standards. J. Clin. Microbiol. 36:3590-3594.
15. Rosenstraus, M., Z. Wang, S. Y. Chang, D. DeBonville, and J. P. Spadoro. 1998. An internal control for routine diagnostic PCR: design, properties, and effect on clinical performance. J. Clin. Microbiol. 36:191-197.
16. Sambrook, J., and D. W. Russell. 2001. Cloning PCR products by addition of restriction sites to the termini of amplified DNA, p. 8.37-8.41. In J. Argentine (ed.), Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
17. Simione, F. P., Jr. 1992. Key issues relating to the genetic stability and preservation of cells and cell banks. J. Parenter. Sci. Technol. 46:226-232.
18. Steel, R. G. D., J. H. Torrie, and D. A. Dickey. 1997. Principles and procedures of statistics: a biometrical approach. McGraw-Hill Companies, Inc., New York, N.Y.
19. Wang, A. M., M. V. Doyle, and D. F. Mark. 1989. Quantitation of mRNA by the polymerase chain reaction. Proc. Natl. Acad. Sci. USA 86:9717-9721.
20. Wright, A. D., and T. J. Crease. 1996. Detection of "lost" plasmids from Escherichia coli using excess ampicillin. Anal. Biochem. 236:181-182.