Unexpected observation of concentration-dependent dissociation rates for antibody-antigen complexes and other macromolecular complexes in competition experiments

Journal of Immunological Methods

Volumn 276, Issue 1-2, 2003, Pages 129-134

  Scheuermann, Jörg ^a   Viti, Francesca ^b   Neri, Dario ^a  

^a ETH ZURICH   (Switzerland)

^b Philogen SpA   (Italy)

Author keywords

Antibody; Antigen; Calmodulin; Competition experiments; EDB domain of fibronectin; Kinetic dissociation constant

Indexed keywords

ANTIGEN; CALMODULIN; PEPTIDE; RECOMBINANT ANTIBODY;

ANTIBODY AFFINITY; ANTIGEN ANTIBODY COMPLEX; ARTICLE; COMPETITION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DILUTION; DISSOCIATION; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; MACROMOLECULE; PRIORITY JOURNAL;

EID: 0242585352     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(03)00060-7     Document Type: Article

References (12)

1. Adams G.P., Schier R., McCall A.M., Simmons H.H., Horak E.M., Alpaugh R.K., Marks J.D., Weiner L.M. High affinity restricts the localization and tumor penetration of single-chain fv antibody molecules. Cancer Res. 61:2001;4750-4755.
2. Borsi L., Balza E., Bestagno M., Castellani P., Carnemolla B., Biro A., Leprini A., Sepulveda J., Burrone O., Neri D., Zardi L. Selective targeting of tumoral vasculature: comparison of different formats of an antibody (L19) to the ED-B domain of fibronectin. Int. J. Cancer. 102:2002;75-85.
3. Elia G., Silacci M., Bumke M.A., Scheurer S., Neri D. Visualizing the proteome in structurally intact samples. Bio World. 3:2002;16-17.
4. Huston J.S., Levinson D., Mudgett-Hunter M., Tai M.S., Novotny J., Margolies M.N., Ridge R.J., Bruccoleri R.E., Haber E., Crea R.et al. Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 85:1988;5879-5883.
5. Montigiani S., Neri G., Neri P., Neri D. Alanine substitutions in calmodulin-binding peptides result in unexpected affinity enhancement. J. Mol. Biol. 258:1996;6-13.
6. Neri D., Momo M., Prospero T., Winter G. High-affinity antigen binding by chelating recombinant antibodies (CRAbs). J. Mol. Biol. 246:1995;367-373.
7. Neri D., Montigiani S., Kirkham P.M. Biophysical methods for the determination of antibody-antigen affinities. Trends Biotechnol. 14:1996;465-470.
8. Neri D., Petrul H., Winter G., Light Y., Marais R., Britton K.E., Creighton A.M. Radioactive labeling of recombinant antibody fragments by phosphorylation using human casein kinase II and [gamma-32P]-ATP. Nat. Biotechnol. 14:1996;485-490.
9. Pini A., Viti F., Santucci A., Carnemolla B., Zardi L., Neri P., Neri D. Design and use of a phage display library. Human antibodies with subnanomolar affinity against a marker of angiogenesis eluted from a two-dimensional gel. J. Biol. Chem. 273:1998;21769-21776.
10. Reynaldo L.P., Vologodskii A.V., Neri B.P., Lyamichev V.I. The kinetics of oligonucleotide replacements. J. Mol. Biol. 297:2000;511-520.
11. Santimaria M., Moscatelli G., Viale G.L., Giovannoni L., Neri G., Viti F., Leprini A., Borsi L., Castellani P., Zardi L., Neri D., Riva P. Immunoscintigraphic detection of the ED-B domain of fibronectin, a marker of angiogenesis, in patients with cancer. Clin. Cancer Res. 9:2003;571-579.
12. Schreiber G., Fersht A.R. Interaction of barnase with its polypeptide inhibitor barstar studied by protein engineering. Biochemistry. 32:1993;5145-5150.