Attenuation of photobleaching in two-photon excitation fluorescence from green fluorescent protein with shaped excitation pulses

Biochemical and Biophysical Research Communications

Volumn 311, Issue 3, 2003, Pages 592-596

  Kawano, Hiroyuki ^a,b   Nabekawa, Yasuo ^a   Suda, Akira ^a   Oishi, Yu ^a   Mizuno, Hideaki ^b   Miyawaki, Atsushi ^b   Midorikawa, Katsumi ^a  

^a RIKEN   (Japan)

^b RIKEN BRAIN SCIENCE INSTITUTE   (Japan)

Author keywords

Femtosecond laser pulse; Fused silica spatial light modulator; Green fluorescent protein; Pulse shaping; Two photon excitation fluorescence microscopy

Indexed keywords

DYE; GREEN FLUORESCENT PROTEIN;

ARTICLE; BLEACHING; EXCITATION; FLUORESCENCE; INTERMETHOD COMPARISON; LASER; OSCILLATOR; PHOTON; PRIORITY JOURNAL; SIGNAL PROCESSING;

EID: 0242577592     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2003.09.236     Document Type: Article

References (15)

1. Denk W. Two-photon scanning photochemical microscopy: mapping ligand-gated ion channel distributions. Proc. Natl. Acad. Sci. USA. 91:1994;6629-6633.
2. Patterson G.H., Piston D.W. Photobleaching in two-photon excitation microscopy. Biophys. J. 78:2000;2159-2162.
3. Chen T.-S., Zeng S.-Q., Luo Q.-M., Zhang Z.-H., Zhou W. High-order photobleaching of green fluorescent protein inside live cells in two-photon excitation microscopy. Biophys. Biochem. Res. Commun. 291:2002;1272-1275.
4. Meshulach D., Silberberg Y. Coherent quantum control of two-photon transitions by a femtosecond laser pulse. Nature. 396:1998;239-242.
5. Meshulach D., Silberberg Y. Coherent quantum control of multiphoton transitions by shaped ultrashort optical pulses. Phys. Rev. A. 60:1999;1287-1292.
6. Dudovich N., Dayan B., Gallagher Faeder S.M., Silberberg Y. Transform-limited pulses are not optimal for resonant multiphoton transitions. Phys. Rev. Lett. 86:2001;47-50.
7. Lee S.H., Jung K.H., Sung J.H., Hong K.H., Nam C.H. Adaptive quantum control of DCM fluorescence in the liquid phase. J. Chem. Phys. 117:2002;9858-9861.
8. Suda A., Oishi Y., Nagasaka K., Wang P., Midorikawa K. A spatial light modulator based on fused-silica plates for adaptive feedback control of intense femtosecond laser pulses. Opt. Express. 9:2001;2-6.
9. Kane D.J., Trebino R. Characterization of arbitrary femtosecond pulses using frequency-resolved optical gating. IEEE J. Quantum Electron. 29:1993;571-579.
10. Dmitriev V.G., Gurzadyan G.G., Nikogosyan D.N. Handbook of Nonlinear Optical Crystals. third ed. 1999;Springer, Heidelberg.
11. Tsien R.Y. The green fluorescent protein. Annu. Rev. Biochem. 67:1998;509-544.
12. Bardeen C.J., Yakovlev V.V., Squier J.A., Wilson K.R. Quantum control of population transfer in green fluorescent protein by using chirped femtosecond pulses. J. Am. Chem. Soc. 120:1998;13023-13027.
13. Ando R., Hama H., Yamamoto-Hino M., Mizuno H., Miyawaki A. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. USA. 99:2002;12651-12656.
14. Nagai T., Ibata K., Park E.S., Kubota M., Mikoshiba K., Miyawaki A. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotech. 20:2002;87-90.
15. Miyawaki A., Tsien R.Y. Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein. Methods Enzymol. 327:2000;472-501.