Enzymatic reaction of silent substrates: Kinetic theory and application to the serine protease chymotrypsin

Biochemistry

Volumn 42, Issue 16, 2003, Pages 4727-4732

  Case, April ^a   Huskey, W Phillip ^b   Stein, Ross L ^a  

^a The Harvard Center for Neurodegeneration & Repair   (United States)

^b RUTGERS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROLYSIS; LEAST SQUARES APPROXIMATIONS; SUBSTRATES; VELOCITY CONTROL;

PHYSIOCHEMICAL PROPERTIES;

ENZYME KINETICS;

CHYMOTRYPSIN; SERINE PROTEINASE;

ARTICLE; ENZYME BINDING; ENZYME KINETICS; ENZYME MECHANISM; ENZYME METABOLISM; ENZYME SUBSTRATE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN HYDROLYSIS; STEADY STATE;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHYMOTRYPSIN; HYDROLYSIS; KINETICS; MODELS, CHEMICAL; OLIGOPEPTIDES; SUBSTRATE SPECIFICITY;

EID: 0242432358     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0207162     Document Type: Article

References (7)

1. Fersht, A. R. (1999) in Structure and Mechanism in Protein Science, pp 191-199, W. H. Freeman, New York.
2. Copeland, R. A. (2000) in Enzymes - A Practical Introduction to Structure, Mechanism, and Data Analysis, pp 188-223, John Wiley & Sons, New York.
3. Harrison, R. K., Teahan, J., and Stein, R. L. (1989) Anal. Biochem. 80, 110-113.
4. Case, A., and Stein, R. L. (2003) Biochemistry 42, 3335-3348.
5. Roberge, M., Santell, L., Dennis, M. S., Eigenbrot, C., Dwyer, M. A., and Lazarus, R. A. (2001) Biochemistry 40, 9522-9531.
6. Monteiro, R. Q., Raposo, J. G., Wisner, A., Guimaraes, J. A., Bon, C., and Zingali, R. B. (1999) Biochem. Biophys. Res. Commun. 262, 819-822.
7. Fuentes-Prior, P., Noeske-Jungblut, C., Donner, P.R, Schleuning, W. D., Huber, R., and Bode, W. (1997) Proc. Natl. Acad. Sci. U.S.A. 94, 11845-11850.