Functional analysis of a PcsB-deficient mutant of group B streptococcus

FEMS Microbiology Letters

Volumn 221, Issue 1, 2003, Pages 73-79

  Reinscheid, Dieter J ^a   Ehlert, Kerstin ^b   Chhatwal, Gursharan S ^c   Eikmanns, Bernhard J ^a  

^a UNIVERSITY OF ULM   (Germany)

^b BAYER AG   (Germany)

^c HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

Muropeptides; Peptidoglycan composition; Streptococcus agalactiae

Indexed keywords

BACTERIAL PROTEIN; CEFOTAXIME; COTRIMOXAZOLE; ENOLASE; IMIPENEM; OFLOXACIN; PENICILLIN G; VANCOMYCIN;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL STRAIN; BACTERIUM MUTANT; CELL LEVEL; CELL MEMBRANE; CELL WALL; CONTROLLED STUDY; GENE EXPRESSION; GENE OVEREXPRESSION; GENE SEQUENCE; GENE TRANSLOCATION; GENETIC TRANSCRIPTION; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PHENOTYPE; PLASMID; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEFICIENCY; PROTEIN SECRETION; REGULATORY MECHANISM; RNA TRANSLATION; STREPTOCOCCUS AGALACTIAE; SUPERNATANT; WILD TYPE;

BACTERIA (MICROORGANISMS); STREPTOCOCCUS; STREPTOCOCCUS AGALACTIAE; STREPTOCOCCUS SP. 'GROUP B';

EID: 0242417047     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00167-8     Document Type: Article

References (33)

1. Baker, C.J. and Edwards, M.S. (1995) Group B streptococcal infections. In: Infectious Diseases of the Fetus and Newborn Infant (Remington, J.S. and Klein, J.O., Eds.), pp. 980-1054. W.B. Saunders, Philadelphia, PA.
2. Baker C.J., Paoletti L.C., Rench M.A., Guttormsen H.K., Carey V.J., Hickman M.E., Kasper D.L. Use of capsular polysaccharide-tetanus toxoid conjugate vaccine for type II group B Streptococcus in healthy women. J. Infect. Dis. 182:2000;1129-1138.
3. Bergmann S., Rohde M., Chhatwal G.S., Hammerschmidt S. alpha-enolase of Streptococcus pneumoniae is a plasmin(ogen)-binding protein displayed on the bacterial cell surface. Mol. Microbiol. 40:2001;1273-1287.
4. Chia J.S., Chang L.Y., Shun C.T., Chang Y.Y., Tsay Y.G., Chen J.Y. A 60-kilodalton immunodominant glycoprotein is essential for cell wall integrity and the maintenance of cell shape in Streptococcus mutans. Infect. Immun. 69:2001;6987-6998.
5. Chia J.S., Lee Y.Y., Huang P.T., Chen J.Y. Identification of stress-responsive genes in Streptococcus mutans by differential display reverse transcription-PCR. Infect. Immun. 69:2001;2493-2501.
6. Dubendorff J.W., Studier F.W. Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor. J. Mol. Biol. 219:1991;45-59.
7. Fagan P.K., Reinscheid D., Gottschalk B., Chhatwal G.S. Identification and characterization of a novel secreted immunoglobulin binding protein from group a streptococcus. Infect. Immun. 69:2001;4851-4857.
8. Glaser P., Rusniok C., Buchrieser C., Chevalier F., Frangeul L., Msadek T., Zouine M., Couve E., Lalioui L., Poyart C., Trieu-Cuot P., Kunst F. Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease. Mol. Microbiol. 45:2002;1499-1513.
9. Hakenbeck R., Konig A., Kern I., van der Linden M., Keck W., Billot-Klein D., Legrand R., Schoot B., Gutmann L. Acquisition of five high-Mr penicillin-binding protein variants during transfer of high-level beta-lactam resistance from Streptococcus mitis to Streptococcus pneumoniae. J. Bacteriol. 180:1998;1831-1840.
10. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:1985;557-580.
11. Hughes M.J., Moore J.C., Lane J.D., Wilson R., Pribul P.K., Younes Z.N., Dobson R.J., Everest P., Reason A.J., Redfern J.M., Greer F.M., Paxton T., Panico M., Morris H.R., Feldman R.G., Santangelo J.D. Identification of major outer surface proteins of Streptococcus agalactiae. Infect. Immun. 70:2002;1254-1259.
12. Hunter T.H. Use of streptomycin in the treatment of bacterial endocarditis. Am. J. Med. 2:1947;436-442.
13. Kasper D.L. Designer vaccines to prevent infections due to group B Streptococcus. Proc. Assoc. Am. Physicians. 107:1995;369-373.
14. Kling D.E., Madoff L.C., Michel J.L. Subcellular fractionation of group B Streptococcus. BioTechniques. 27:1999;24-28.
15. Leclercq R. Enterococci acquire new kinds of resistance. Clin. Infect. Dis. 24:1997;S80-S84.
16. Lei B., Mackie S., Lukomski S., Musser J.M. Identification and immunogenicity of group A Streptococcus culture supernatant proteins. Infect. Immun. 68:2000;6807-6818.
17. Mattos-Graner R.O., Jin S., King W.F., Chen T., Smith D.J., Duncan M.J. Cloning of the Streptococcus mutans gene encoding glucan binding protein B and analysis of genetic diversity and protein production in clinical isolates. Infect. Immun. 69:2001;6931-6941.
18. Mazmanian S.K., Liu G., Ton-That H., Schneewind O. Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall. Science. 285:1999;760-763.
19. Moellering R.C. The enterococcus: a classic example of the impact of antimicrobial resistance on therapeutic options. J. Antimicrob. Chemother. 28:1991;1-12.
20. Pancholi V., Fischetti V.A. A major surface protein on group A streptococci is a glyceraldehyde-3-phosphate-dehydrogenase with multiple binding activity. J. Exp. Med. 176:1992;415-426.
21. Pancholi V., Fischetti V.A. alpha-Enolase, a novel strong plasmin(ogen) binding protein on the surface of pathogenic streptococci. J. Biol. Chem. 273:1998;14503-14515.
22. Paoletti L.C., Pinel J., Johnson K.D., Reinap B., Ross R.A., Kasper D.L. Synthesis and preclinical evaluation of glycoconjugate vaccines against group B Streptococcus types VI and VIII. J. Infect. Dis. 180:1999;892-895.
23. Paoletti L.C., Pinel J., Kennedy R.C., Kasper D.L. Maternal antibody transfer in baboons and mice vaccinated with a group B streptococcal polysaccharide conjugate. J. Infect. Dis. 181:2000;653-658.
24. Reinscheid D.J., Gottschalk B., Schubert A., Eikmanns B.J., Chhatwal G.S. Identification and molecular analysis of PcsB, a protein required for cell wall separation of group B streptococcus. J. Bacteriol. 183:2001;1175-1183.
25. Reinscheid D.J., Stoesser C., Moeller K., Ehlert K., Jack R.W., Eikmanns B.E., Chhatwal G.S. Influence of proteins Bsp and FemH on cell shape and peptidoglycan composition in group B streptococcus. Microbiology. 148:2002;3245-3254.
26. Ricci M.L., Manganelli R., Berneri C., Orefici G., Pozzi G. Electrotransformation of Streptococcus agalactiae with plasmid DNA. FEMS Microbiol. Lett. 119:1994;47-52.
27. Rubens, C.E., Kuypers, J.M., Heggen, L.M., Kasper, D.L. and Wessels, M.R. (1991) Molecular analysis of the group B streptococcal capsule genes. In: Lactococci, Enterococci and Streptococci (Dunny, G.M., Cleary, P.P. and McKay, L.L., Eds.), pp. 179-183. American Society for Microbiology, Washington, DC.
28. Sambrook, J., Fritsch, E. F. and Maniatis, J. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor Laboratory, NY.
29. Schubert K., Bichlmaier A.M., Mager E., Wolff K., Ruhland G., Fiedler F. P45, an extracelular 45 kDa protein of Listeria monocytogenes with similarity to protein p60 and exhibiting peptidoglycan lytic activity. Arch. Microbiol. 173:2000;21-28.
30. Tettelin H., Masignani V., Cieslewicz M.J., Eisen J.A., Peterson S., Wessels M.R., Paulsen I.T., Nelson K.E., Margarit I., Read T.D., Madoff L.C., Wolf A.M., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Lewis M.R., Radune D., Fedorova N.B., Scanlan D., Khouri H., Mulligan S., Carty H.A., Cline R.T., Van Aken S.E., Gill J., Scarselli M., Mora M., Iacobini E.T., Brettoni C., Galli G., Mariani M., Vegni F., Maione D., Rinaudo D., Rappuoli R., Telford J.L., Kasper D.L., Grandi G., Fraser C.M. Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae. Proc. Natl. Acad. Sci. USA. 99:2002;12391-12396.
31. Trieu-Cuot P., Poyart-Salmeron C.C., Courvalin P. A pair of mobilizable shuttle vectors conferring resistance to spectinomycin for molecular cloning in Escherichia coli and in gram-positive bacteria. Nucleic Acids Res. 18:1990;4296.
32. Valentin-Weigand P., Benkel P., Rohde M., Chhatwal G.S. Entry and intracellular survival of group B streptococci in J774 macrophages. Infect. Immun. 64:1996;2467-2473.
33. Weber B., Ehlert K., Diehl A., Reichmann P., Labischinski H., Hakenbeck R. The fib locus in Streptococcus pneumoniae is required for peptidoglycan crosslinking and PBP-mediated beta-lactam resistance. FEMS Microbiol. Lett. 188:2000;81-85.