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Volumn 125, Issue 46, 2003, Pages 14093-14102

Protein-Based Radicals in the Catalase-Peroxidase of Synechocystis PCC6803: A Multifrequency EPR Investigation of Wild-Type and Variants on the Environment of the Heme Active Site

Author keywords

[No Author keywords available]

Indexed keywords

AROMATIC COMPOUNDS; BIOCHEMISTRY; CATALYSIS; ENZYME KINETICS; FREE RADICALS; HYDROGEN BONDS; MUTAGENESIS; PARAMAGNETIC RESONANCE; REACTION KINETICS; SPECTROSCOPY;

EID: 0242414727     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja035582+     Document Type: Article
Times cited : (103)

References (54)
  • 10
    • 0242478835 scopus 로고    scopus 로고
    • note
    • Each subunit in catalase-peroxidase is constituted by two structurally similar domains, the N-terminal and the C-terminal domains that mainly difference being the absence of the heme cofactor in the C-terminal domain (refs 4 and 8).
  • 34
    • 2942732763 scopus 로고
    • Lever, A. B. P., Gray, H. B., Eds; Adison-Wesley Publishing Co.: Reading, MA
    • Palmer, G. In Iron Porphyrins, part 11; Lever, A. B. P., Gray, H. B., Eds; Adison-Wesley Publishing Co.: Reading, MA, 1983; pp 43-88.
    • (1983) Iron Porphyrins, Part 11 , pp. 43-88
    • Palmer, G.1
  • 39
    • 0242395413 scopus 로고    scopus 로고
    • note
    • .).
  • 42
    • 0242478832 scopus 로고    scopus 로고
    • note
    • The H123Q HF EPR spectrum exhibited unusually broad shape. The EPR spectrum recorded at 190 GHz was identical in shape to the 285-GHz spectrum when plotted as a function of g-values, This indicated that the shape was due to distribution in g-tensor. Further work is require to understand the nature of this spectrum.
  • 45
    • 0035955208 scopus 로고    scopus 로고
    • 2 group served to retain the H-bond to the Asn82. If one assumes that the mutation of the distal histidine (H123Q) to a glutamine in catalase-peroxidase results in the same arrangement as the glutamine in the comparable CcP variant, the decreased basicity of the glutamine carbonyl relative to imidazole is likely to contribute to the "inverted" pH effect observed on the EPR spectrum of the H123Q variant.
    • (2001) J. Am. Chem. Soc. , vol.123 , pp. 9260-9263
    • Bateman, L.1    Léger, C.2    Goodin, D.B.3    Armstrong, F.A.4
  • 52
    • 0242647204 scopus 로고    scopus 로고
    • note
    • The dihedral angles of the β-protons are similar, as judged from the crystallographic structure (ref 4). Hence, the β-protons hyperfine couplings would be of little help to decide between the Trp122 and Trp106.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.