Protistan Grazing Analysis by Flow Cytometry Using Prey Labeled by in Vivo Expression of Fluorescent Proteins

Applied and Environmental Microbiology

Volumn 69, Issue 11, 2003, Pages 6848-6855

  Fu, Yutao ^a,c   O'Kelly, Charles ^a   Sieracki, Michael ^a   Distel, Daniel L ^a,b  

^a BIGELOW LABORATORY FOR OCEAN SCIENCES   (United States)

^b UNIVERSITY OF MAINE   (United States)

^c BOSTON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; ESCHERICHIA COLI; FLUORESCENCE; PROTEINS;

FLOW CYTOMETRY;

BACTERIOLOGY;

GREEN FLUORESCENT PROTEIN; RED FLUORESCENT PROTEIN;

ALGA; ANALYTIC METHOD; ARTICLE; AUTOFLUORESCENCE; DNA MODIFICATION; ENTEROBACTER AEROGENES; ESCHERICHIA COLI; FLAGELLATE; FLOW CYTOMETRY; MICROMONAS PUSILLA; NONHUMAN; PARAPHYSOMONAS IMPERFORATA; PLASMID; PREY; PROTEIN EXPRESSION; PSEUDOMONAS PUTIDA;

ANIMALS; FLOW CYTOMETRY; FLUORESCEINS; GRAM-NEGATIVE BACTERIA; GREEN FLUORESCENT PROTEINS; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; MASTIGOPHORA; PLASMIDS; PREDATORY BEHAVIOR; STAINING AND LABELING;

ALGAE; BACTERIA (MICROORGANISMS); BROAD HOST RANGE PLASMIDS; ENTEROBACTER; ENTEROBACTER AEROGENES; ESCHERICHIA COLI; MASTIGOPHORA (FLAGELLATES); MICROMONAS PUSILLA; NEGIBACTERIA; PARAPHYSOMONAS; PARAPHYSOMONAS IMPERFORATA; PROTISTA; PROTOZOA; PSEUDOMONAS; PSEUDOMONAS PUTIDA;

EID: 0242405582     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.11.6848-6855.2003     Document Type: Article

References (33)

1. Boenigk, J., and H. Arndt. 2002. Bacterivory by heterotrophic flagellates: community structure and feeding strategies. Antonie Leeuwenhoek 81:465-480.
2. Christaki, U., C. Courties, H. Karayanni, A. Giannakourou, C. Maravelias, K. A. Kormas, and P. Lebaron. 2002. Dynamic characteristics of Prochlorococcus and Synechococcus consumption by bacterivorous nanoflagellates. Microb. Ecol. 43:341-352.
3. Christoffersen, K., O. Nybroe, K. Jurgens, and M. Hansen. 1997. Measurement of bacterivory by heterotrophic nanoflagellates using immunofluorescence labeling of ingested cells. Aquat. Microb. Ecol. 13:127-134.
4. Daubaras, D. A. M. C. 1992. The environment, microbes, and bioremediation: microbial activities modulated by the environment. Biodegradation 3:125-135.
5. Ellis, L. B., C. D. Hershberger, and L. P. Wackett. 2000. The University of Minnesota Biocatalysis/Biodegradation database: microorganisms, genomics, and prediction. Nucleic Acids Res. 28:377-379.
6. Epstein, S. S., and J. Rossel. 1995. Methodology of in situ grazing experiments: evaluation of a new vital dye for preparation of fluorescently labeled bacteria. Mar Ecol. Prog. Ser. 128:143-150.
7. Errampalli, D., K. Leung, M. B. Cassidy, M. Kostrzynska, M. Blears, H. Lee, and J. T. Trevors. 1999. Applications of the green fluorescent protein as a molecular market in environmental microorganisms. J. Microbiol. Methods 35:187-199.
8. Evaristo Vazquez-Dominguez, F. P., J. M. Gasol, and D. Vaqué. 1999. Measuring the grazing losses of picoplankton: methodological improvements in the use of fluorescently labeled tracers combined with flow cytometry. Aquatic Microb. Ecol. 20:119-128.
9. Guillard, R. R. L., and P. E. Hargraves. 1993. Stichochrysis immobilis is a diatom, not a chyrsophyte. Phycologia 32:234-236.
10. Hahn, M. W., and M. G. Hofle. 1999. Flagellate predation on a bacterial model community: interplay of size-selective grazing, specific bacterial cell size, and bacterial community composition. Appl. Environ. Microbiol. 65:4863-4872.
11. Hahn, M. W., and M. G. Hofle. 1998. Grazing pressure by a bacterivorous flagellate reverses the relative abundance of Comamonas acidovorans PX54 and Vibrio strain CB5 in chemostat cocultures. Appl. Environ. Microbiol. 64:1910-1918.
12. Jurgens, K., and C. Matz. 2002. Predation as a shaping force for the phenotypic and genotypic composition of planktonic bacteria. Antonie Leeuwenhoek 81:413-434.
13. Jurgens, K., J. Pernthaler, S. Schalla, and R. Amann. 1999. Morphological and compositional changes in a planktonic bacterial community in response to enhanced protozoan grazing. Appl. Environ. Microbiol. 65:1241-1250.
14. Kinner, N. E., R. W. Harvey, K. Blakeslee, G. Novarino, and L. D. Meeker. 1998. Size-selective predation on groundwater bacteria by nanoflagellates in an organic-contaminated aquifer. Appl. Environ. Microbiol. 64:618-625.
15. Leung, K. T., J. S. So, M. Kostrzynska, H. Lee, and J. T. Trevors. 2001. Using a green fluorescent protein gene-labeled p-nitrophenol-degrading Moraxella strain to examine the protective effect of alginate encapsulation against protozoan grazing. J. Microbiol. Methods 39:205-211.
16. Lowder, M., A. Unge, N. Maraha, J. K. Jansson, J. Swiggett, and J. D. Oliver. 2000. Effect of starvation and the viable-but-nonculturable state on green fluorescent protein (GFP) fluorescence in GFP-tagged Pseudomonas fluorescens A506. Appl. Environ. Microbiol. 66:3160-3165.
17. Nygaard, K., and D. O. Hessen. 1990. Use of C-14 protein-labeled bacteria for estimating clearance rates by heterotrophic and mixotrophic flagellates. Mar Ecol. Prog. Ser. 68:7-14.
18. Parry, J. D., K. Heaton, J. Drinkall, and H. L. Jones. 2001. Feasibility of using GFP-expressing Escherichia coli, coupled with fluorimetry, to determine protozoan ingestion rates. FEMS Microbiol. Ecol. 35:11-17.
19. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
20. Shapiro, H. M. 1995. Practical flow cytometry, 3rd ed. Wiley-Liss, New York, N.Y.
21. Sherr, B. F., E. B. Sherr, and R. D. Fallon. 1987. Use of monodispersed fluorescently labeled bacteria to estimate in situ protozoan bacterivory. Appl. Environ. Microbiol. 53:958-965.
22. Sherr, B. F., E. B. Sheer, and T. Berman. 1982. Decomposition of organic detritus: a selective role for microflagellate protozoa. Limnol. Oceanogr. 27:765-769.
23. Sherr, E. B., and B. F. Sherr. 2002. Significance of predation by protists in aquatic microbial food webs. Antonie Leeuwenhoek 81:293-308.
24. Sibille, I., T. Sime-Ngando, L. Mathieu, and J. C. Block. 1998. Protozoan bacterivory and Escherichia coli survival in drinking water distribution systems. Appl. Environ. Microbiol. 64:197-202.
25. Sieracki., M. E., L. W. Haas, D. A. Caron, and E. J. Lessard. 1987. Effect of fixation on particle retention by microflagellates: underestimation of grazing rates. Mar. Ecol. Prog. Ser 38:251-258.
26. Simek, K., J. Armengol, M. Comerma, J. C. Garcia, P. Kojecka, J. Nedoma, and J. Hejzlar. 2001. Changes in the epilimnetic bacterial community composition, production, and protist-induced mortality along the longitudinal axis of a highly eutrophic reservoir. Microb. Ecol. 42:359-371.
27. Simek, K., and T. H. Chrzanowski. 1992. Direct and indirect evidence of size-selective grazing on pelagic bacteria by fresh-water nanoflagellates. Appl. Environ. Microbiol. 58:3715-3720.
28. Simek, K., J. Nedoma, J. Pernthaler, T. Posch, and J. R. Dolan. 2002. Altering the balance between bacterial production and protistan bacterivory triggers shifts in freshwater bacterial community composition. Antonie Leeuwenhoek 81:453-463.
29. Simek, K., J. Pernthaler, M. G. Weinbauer, K. Hornak, J. R. Dolan, J. Nedoma, M. Masin, and R. Amann. 2001. Changes in bacterial community composition and dynamics and viral mortality rates associated with enhanced flagellate grazing in a mesoeutrophic reservoir. Appl. Environ. Microbiol. 67:2723-2733.
30. Sin, Y., K. L. Webb, and M. E. Sieracki. 1998. Carbon and nitrogen densities of the cultured marine heterotrophic flagellate Paraphysomonas sp. J. Microbiol. Methods 34:151-163.
31. Tso, S. F., and G. L. Taghon. 1997. Enumeration of protozoa and bacteria in muddy sediment. Microb. Ecol. 33:144-148.
32. Verity, P. G. 1991. Feeding in planktonic protozoans: evidence for nonrandom acquisition of prey. J. Protozool. 38:69-76.
33. Weisse, T. 2002. The significance of inter- and intraspecific variation in bacterivorous and herbivorous protists. Antonie Leeuwenhoek 81:327-341.