Using a green fluorescent protein gene-labeled p-nitrophenol-degrading Moraxella strain to examine the protective effect of alginate encapsulation against protozoan grazing

Journal of Microbiological Methods

Volumn 39, Issue 3, 2000, Pages 205-211

  Leung, Kam Tin ^a   So, Jae Seong ^b   Kostrzynska, Magdalena ^a   Lee, Hung ^a   Trevors, Jack T ^a  

^a UNIVERSITY OF GUELPH   (Canada)

^b Inha University   (South Korea)

Author keywords

Alginate encapsulation; Green fluorescent protein; Moraxella sp.; Protozoan grazing

Indexed keywords

4 NITROPHENOL; ALGINIC ACID; GREEN FLUORESCENT PROTEIN;

ARTICLE; BIODEGRADATION; CELL VACUOLE; CONTROLLED STUDY; ENCAPSULATION; FLUORESCENCE MICROSCOPY; MORAXELLA; NONHUMAN; POPULATION DYNAMICS; PRIORITY JOURNAL; PROTECTION; PROTOZOON; SCANNING ELECTRON MICROSCOPY; TETRAHYMENA THERMOPHILA;

ALGINATES; ANIMALS; BACTERIAL CAPSULES; COLONY COUNT, MICROBIAL; GENETIC MARKERS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MORAXELLA; NITROPHENOLS; TETRAHYMENA;

BACTERIA (MICROORGANISMS); MORAXELLA SP.; PROTOZOA; TETRAHYMENA THERMOPHILA;

EID: 0033986846     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(99)00120-7     Document Type: Article

References (22)

1. Acea M., Moore C.R., Alexander M. Survival and growth of bacteria introduced into soil. Soil Biol. Biochem. 20:1988;509-515.
2. Berninger U.-G., Finlay B.J., Kuuppo-Leinikki P. Protozoan control of bacterial abundances in freshwater. Limnol. Oceanogr. 36:1991;139-147.
3. Casida L.E. Protozoan response to the addition of bacterial predators and other bacteria to soil. Appl. Environ. Microbiol. 55:1989;1857-1859.
4. Cassidy M.B., Lee H., Trevors J.T. Environmental applications of immobilized microbial cells: a review. J. Ind. Microbiol. 16:1996;79-101.
5. Danso S.K.A., Keya S.O., Alexander M. Protozoa and the decline of Rhizobium populations added to soil. Can. J. Microbiol. 21:1975;884-895.
6. Eisenmann H., Harms H., Meckenstock R., Meyer E.I., Zehnder A.J.B. Grazing of a Tetrahymena sp. on adhered bacteria in percolated columns monitored by in situ hybridization with fluorescent oligonucleotide probes. Appl. Environ. Microbiol. 64:1998;1264-1269.
7. England L.S., Lee H., Trevors J.T. Bacterial survival in soil: effect of clays and protozoa. Soil Biol. Biochem. 25:1993;525-531.
8. Habte M., Alexander M. Further evidence for the regulation of bacterial populations in soil by protozoa. Arch. Microbiol. 113:1977;181-183.
9. Heynen C.E., van Elsas J.D., Kuikman P.J., van Veen J.A. Dynamics of Rhizobium leguminosarum biovar trifolii introduced into soil; the effect of bentonite clay on predation by protozoa. Soil Biol. Biochem. 20:1988;483-488.
10. Kwaik Y.A., Gao L.-Y., Stone B.J., Venkataraman C., Harb O.S. Invasion of protozoa by Legionella pneumophila and its role in bacterial ecology and pathogenesis. Appl. Environ. Microbiol. 64:1998;3127-3133.
11. Madsen E.L., Sinclair J.L., Ghiorse W.C. In situ biodegradation: microbiological patterns in a contaminated aquifer. Science. 252:1991;830-833.
12. Novitsky J.A. Protozoa abundance, growth, and bacterivory in the water column, on sedimenting particles, and in the sediment of Halifax Harbor. Can. J. Microbiol. 36:1990;859-863.
13. Nygaard K., Borsheim K.Y., Thingstad T.F. Grazing rates on bacteria by marine heterotrophic microflagellates compared to uptake rates of bacterial-sized monodisperse fluorescent latex beads. Mar. Ecol. Prog. Ser. 44:1988;159-165.
14. Pritchard P.H. Use of inoculation in bioremediation. Curr. Opin. Biotechnol. 3:1992;232-243.
15. Sherr B.F., Sherr E.B. High rates of consumption of bacteria by pelagic ciliates. Nature (Lond.). 325:1987;710-711.
16. Sherr B.F., Sherr E.B., Fallon R.D. Use of monodispersed, fluorescently labeled bacteria to estimate in situ protozoan bacterivory. Appl. Environ. Microbiol. 53:1987;958-965.
17. Sinclair J.L., Ghiorse W.C. Distribution of protozoa in subsurface sediments of a pristine groundwater study site in Oklahoma. Appl. Environ. Microbiol. 53:1987;1157-1163.
18. Spain J.C., Wyss O., Gibson D.T. Enzymatic oxidation of p-nitrophenol. Biochem. Biophys. Res. Commun. 88:1979;634-641.
19. Tresse O., Errampalli D., Kostrzynska M., Leung K.T., Lee H., Trevors J.T. Green fluorescent protein as a visual marker in a p-nitrophenol degrading Moraxella sp. FEMS Microbiol. Lett. 164:1998;187-193.
20. Trevors J.T., van Elsas J.D., Lee H., van Overbeek L.S. Use of alginate and other carriers for encapsulation of microbial cells for use in soil. Microb. Releases. 1:1992;61-69.
21. Trevors J.T., van Elsas J.D., Lee H., Wolters A.C. Survival of alginate-encapsulated Pseudomonas fluorescens cells in soil. Appl. Microbiol. Biotechnol. 39:1993;637-643.
22. Weir S.C., Dupuis S.P., Providenti M.A., Lee H., Trevors J.T. Nutrient-enhanced survival of and phenanthrene mineralization by alginate-encapsulated and free Pseudomonas sp. UG14Lr cells in creosote-contaminated soil slurries. Appl. Microbiol. Biotechnol. 43:1995;946-951.