Superoxide Dismutase Content and Fatty Acid Composition in Subsets of Human Spermatozoa from Normozoospermic, Asthenozoospermic, and Polyzoospermic Semen Samples

Molecular Reproduction and Development

Volumn 66, Issue 4, 2003, Pages 422-430

  Calamera, J ^a   Buffone, M ^a   Ollero, M ^b   Alvarez, J ^b,c   Doncel, G F ^d  

^a Laboratorio de Estudios en Reproduccion LER   (Argentina)

^b HARVARD MEDICAL SCHOOL   (United States)

^c Ctro de Infertil Masc ANDROGEN   (Spain)

^d EASTERN VIRGINIA MEDICAL SCHOOL   (United States)

Author keywords

Antioxidants; ISolate fractions; Maturation; Oxidative stress; Semen pathology; Sperm lipids

Indexed keywords

DOCOSAHEXAENOIC ACID; FATTY ACID; REACTIVE OXYGEN METABOLITE; STEROL; SUPEROXIDE DISMUTASE; UNSATURATED FATTY ACID;

ADULT; ARTICLE; ASTHENOSPERMIA; CELL ISOLATION; CELL STRUCTURE; CELL SUBPOPULATION; CLINICAL ARTICLE; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; HUMAN; HUMAN CELL; LIPID ANALYSIS; LIPID COMPOSITION; LIPID PEROXIDATION; MALE; OXIDATION; POLYSPERMY; PRIORITY JOURNAL; SEMEN ANALYSIS; SPERMATOZOON; SPERMATOZOON MATURATION; SPERMATOZOON MOTILITY;

ADULT; FATTY ACIDS; HUMANS; INFERTILITY, MALE; MALE; SPERM MOTILITY; SPERMATOZOA; SUPEROXIDE DISMUTASE;

EID: 0242322056     PISSN: 1040452X     EISSN: None     Source Type: Journal    
DOI: 10.1002/mrd.10368     Document Type: Article

References (30)

1. Aitken RJ, Harkiss D, Buckingham DW. 1993. Analysis of lipid peroxidation mechanisms in human spermatozoa. Mol Reprod Dev 35:302-315.
2. Aitken RJ, Krausz C, Buckingham D. 1994. Relationships between biochemical markers for residual sperm cytoplasm, reactive oxygen species generation and the presence of leukocytes and precursor germ cells in human sperm suspensions. Mol Reprod Dev 39:268-279.
3. Aitken RJ, Buckingham DW, Carreras A, Irvine DS. 1996. Superoxide dismutase in human sperm suspensions: Relationship with cellular composition, oxidative stress, and sperm function. Free Radic Biol Med 21:495-504.
4. Alvarez JG, Storey BT. 1983. Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility. Biol Reprod 29:548-555.
5. Alvarez JG, Storey BT. 1984. Lipid peroxidation and the reactions of superoxide and hydrogen peroxide in mouse spermatozoa. Biol Reprod 30:833-841.
6. Alvarez JG, Storey BT. 1989. Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation. Gamete Res 23:77-90.
7. Alvarez JG, Storey BT. 1995. Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol Reprod Dev 42:334-346.
8. Alvarez JG, Touchstone JC. 1992. Separation of acidic and neutral lipids by aminopropyl-bonded silica gel column chromatography. J Chomatogr Biomed Appl 577:142-145.
9. Alvarez JG, Touchstone JC, Blasco L, Storey BT. 1987. Spontaneous lipid peroxidation and production of hydrogen peroxide and super-oxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity. J Androl 8:338-348.
10. Alvarez JG, Sharma RK, Ollero M, Saleh RA, Lopez MC, Thomas AJ, Evenson DP, Agarwal A. 2002. Increased DNA damage in sperm from leukocytospermic semen sample as determined by the sperm chromatin structure assay. Fertil Steril 78:319-329.
11. Calamera JC, Giovenco P, Brugo S, Dondero F, Nicholson RF. 1987. Adenosine 5 triphosphate (ATP) content and acrosin activity in polyzoospermic subjects. Andrologia 19:460-463.
12. De Lamirande E, Gagnon C. 1993. A positive role for the superoxide anion in triggering hyperactivation and capacitation of human spermatozoa. Int J Androl 16:21-25.
13. Gavella M, Lipovac V, Vucic M, Rocic B. 1996. Relationship of sperm superoxide dismutase-like activity with other sperm-specific enzimes and experimentally induced lipid peroxidation in infertile men. Andrologia 28:223-229.
14. Gil-Guzman E, Ollero M, Lopez MC, Sharma RK, Alvarez JG, Thomas AJ, Agarwal A. 2001. Differential production of reactive oxygen species by subsets of human spermatozoa at different stage of maturation. Hum Reprod 16:1022-1930.
15. Glezerman N, Bernstein D, Zakut C, Misgav N, Insler V. 1982. Poly-zoospermia a definite pathologic entity. Fertil Steril 38:605-608.
16. Griveau JF, Renard P, Le Lannou D. 1995. Superoxide anion production by human spermatozoa as a part of the ionophore-induced acrosome reaction process. Int J Androl 18:67-74.
17. Huszar G, Vigue L. 1993. Incomplete development of human spermatozoa is associated with increased creatine phosphokinase concentration and abnormal head morphology. Mol Reprod Dev 34:292-298.
18. Jones R, Mann T, Sherings RJ. 1978. Adverse effects of peroxidized lipid on human spermatozoa. Proc R Soc Lond B Biol Sci 201:413-417.
19. Kobayashi T, Miyazaki T, Natori M, Nozawa S. 1991. Predictive role of superoxide dismutase in human sperm motility: Superoxide dismutase activity and lipid peroxide in human seminal plasma and spermatozoa. Hum Reprod 7:987-991.
20. Koshio A, Akanuma Y, Kosuga M. 1988. Hydrogen peroxide stimulates tyrosine phosphorilation of the insulin receptor and its tyrosine kinase activity on intact cells. Biochem J 250:95-101.
21. Kovalski NN, de Lamirande E, Gagnon C. 1992. Reactive oxygen species generated by human neutrophils inhibit sperm motility: Protective effect of seminal plasma and scavengers. Fertil Steril 58:809-815.
22. Kruger TF, Acosta AA, Simmons KF, Swanson JR, Matta JF, Veeck L, Morshedi R, Brugo S. 1987. New method of evaluating sperm morphology with predictive value for IVF. Urology 30:248-253.
23. Levieux A, Levieux D, Lab C. 1991. Immunoquantitation of rat erythrocyte superoxide dismutase: Its use in copper deficiency. Free Radic Biol Med 11:589-595.
24. Li TK. 1975. The glutathione and thiol content of mammalian spermatozoa and seminal plasma. Biol Reprod 12:641-646.
25. Menella MRF, Jones R. 1980. Properties of spermatozoal superoxide dismutase and lack of involvement of superoxides in metals ion-catalyzed lipid peroxidation reactions in semen. Biochem J 191:289-297.
26. Ollero M, Powers RD, Alvarez JG. 2000. Variation in docosahexaenoic acid content in subsets of human spermatozoa at different stage of maturation: Implications for sperm lipoperoxidative damage. Mol Reprod Dev 55:326-334.
27. Ollero M, Gil-Guzman E, Lopez MC, Sharma RK, Agarwal A, Larson K, Evenson D, Thomas AJ, Alvarez JG. 2001. Characterization of subset of human spermatozoa at different stage of maturation: Implications in the diagnosis and treatment of male infertility. Hum Reprod 16:1912-1921.
28. Shill WB. 1990. Determination of active non zymogen acrosin, proacrosin and total acrosin in different andrological patients. Arch Dermatol Res 282:335-342.
29. Twingg J, Fulton N, Gomez E, Irvine DS, Aitken RJ. 1998. Analysis of the impact of intracellular reactive oxygen species generation on the structural and functional integrity of human spermatozoa. Hum Reprod 13:1429-1436.
30. World Health Organization. 1999. Computer-aided sperm analysis. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction, fourth edition. Cambridge: Cambridge University Press. pp 60-61.