Molecular Cloning and Expression in Cryptorchid Testis of SRG2 from a Mouse Testis Spermatocyte Apoptosis-related Gene

Acta Genetica Sinica

Volumn 30, Issue 10, 2003, Pages 943-948

  Liu, Shang Feng ^a   Li, Lu Yun ^a   Mo, Ya Qin ^a   Fu, Jun Jiang ^a   Liu, Gang ^a   Xing, Xiao Wei ^a   Lu, Guang Xiu ^a  

^a CENTRAL SOUTH UNIVERSITY   (China)

Author keywords

Apoptosis; Cryptorchid testis; Gene cloning; Molecular beacon; RT PCR

Indexed keywords

COMPLEMENTARY DNA;

APOPTOSIS; ARTICLE; CRYPTORCHISM; DNA LIBRARY; GENE; GENE CONTROL; ISOELECTRIC POINT; MALE; MALE INFERTILITY; MOLECULAR CLONING; MOLECULAR WEIGHT; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; SPERMATOCYTE; SRG2 GENE; TESTIS; TSARG2 GENE;

AMINO ACID SEQUENCE; ANIMALS; APOPTOSIS; BASE SEQUENCE; CRYPTORCHIDISM; DNA, COMPLEMENTARY; FLUORESCENT DYES; FLUOROMETRY; GENE EXPRESSION; MALE; MICE; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDE PROBES; PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SPERMATOCYTES; TESTIS;

EUKARYOTA; RODENTIA;

EID: 0242299166     PISSN: 03794172     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. ZHENG Hang, ZHENG Xin-Min, LI Shi-Wen, HU Li-Quan, LIU Xiao-Peng. Bcl-2/Bax expression and testicular germ cell apoptosis in experimental cryptorchidism. Chinse Journal of Andrology, 2000, 14 (2): 81-85.
2. Krajewski S, Bodrug S, Krajewska M, Shabaik A, Gascoyne R, Berean K, Reed J C. Immunohistochemical analysis of Mcl ∼ 1 protein in human tissues. Differential regulation of Mcl-1 and Bcl-2 protein production suggests a unique role for Mcl-1 in control of programmed cell death in vivo. Am J Pathol, 1995, 146(6): 1309-1319.
3. Riou G, Barrois M, Prost S, Terrier M J, Theodore C, Levine A J. The p53 and mdm-2 genes in human testicular germ-cell tumors. Mol Carcinog, 1995, 12(3): 124-131.
4. Blendy J A, Kaestner K H, Weinbauer G F, Nieschlag E, Schutz G. Severe impairment of spermatogenesis in mice lacking the CREM gene. Nature, 1996, 380(6570): 162-165.
5. Nagata S, Golstein P. The Fas death factor. Science, 1995, 267 (5203): 1449-1456.
6. Dix D J, Allen J W, Collins B W, Mori C, Nakamura N, Poorman Allen P, Goulding E H, et al. Targeted gene disruption of Hsp70-2 results in failed meiosis, germ cell apoptosis, and male infertility. Proc Natl Acad Sci USA, 1996, 93(8): 3264-3268.
7. Sarge K D, Cullen K E. Regulation of hsp expression during rodent spermatogenesis. Cell Mol Life Sci, 1997, 53(2): 191-197.
8. Green D R, Reed J C. Mitochondria and apoptosis. Science, 1998, 281 (5381): 1309-1312.
9. Bauche F, Stephan J P, Touzalin A M, Jegou B. In vitro regulation of an inducible-type NO synthase in the rat seminiferous tubule cells. Biol Reprod, 1998, 58(2): 431-438.
10. LIU Xiao-Peng, ZHENG Xin-Min, LI Shi-Wen, HU Li-Quan, ZHENG Hang. Testicular torsion/detorsion induced germ cell apoptosis and expression of gene iNOS. Chinese Journal of Andrology, 2001, 15(1): 11-13.
11. Bretz J D, Rymaszewski M, Arscott P L, Myc A, Ain K B, Thompson N W, Baker J R Jr. TRAIL death pathway expression and induction in thyroid follicular cells. J Biol Chem, 1999, 274(33): 23627-23632.
12. Kodaira K, Takahashi R, Hirabayashi M, Suzuki T, Obinata M, Ueda M. Overexpression of c-myc induces apoptosis at the prophase of meiosis of rat primary spermatocytes. Mol Reprod Dev, 1996, 45(4): 403-410.
13. GUO Cai-Xia, HU Zhao-Yuan, ZOU Ru-Jin, MU Xiao-Min, LIU Yi-Xun. The expression of orphan receptor TR2 mRNA in spermatocyte of rat and Macaca mulatta cryptorchidism. Chinese Sci Bull, 1999, 44 (23): 2539-2544.
14. JIANG Hong, LI Lu-Yun, LU Guang-Xiu. Molecular cloning of genes related to apoptosis in spermatogenic cells of mouse. Acta Biochimet Biophys Sinica, 2001, 33(4): 421-425.
15. FU Jun-Jiang, LU Guang-Xiu, LI Lu-Yun, LIU Gang, XING Xiao-Wei, LIU Shang-Feng. Molecular cloning for human and mouse testis spermatogenesis cell apoptosis related-gene, TSARG1 and Mtsargl and expression analysis for Mtsarg1 gene. Acta Genetica Sinica 2003, 30(1): 25-29.
16. Tyagi S, Kramer F R. Molecular Beacon: probes that fluorescence upon hybridization. Nat Biotechnol, 1996, 14(3): 303-308.
17. Fang X, Li J J, Tan W. Using molecular beacons to probe molecular interactions between lactate dehydrogenase and single-stranded DNA. Anal Chem, 2000, 72(14): 3280-3285.
18. Perlette J, Tan W. Real-time monitoring of intracellular mRNA hybridization inside single living cells. Anal Chem, 2002, 73(22): 5544-5550.
19. LIU Shang-Feng, LI Lu-Yun, FU Jun-Jiang, LIU Gang, XING Xiao-Wei, LU Guang-Xiu. Rapid identification of human testis spermatocyte apoptosis-related gene, TSARG2, by nested PCR and draft human genome searching. Acta Biochimet Biophys Sinica, 2002, 34(3): 378-382.